Neurogenin-2 –transduced human neural progenitor cells attenuate neonatal hypoxic-ischemic brain injury

Lee, Il Shin; Koo, Kyo Yeon; Jung, Kwangsoo; Kim, Miri; Kim, Il Sun; Hwang, Kyujin; Yun, Seokhwan; Lee, Hae-Jin; Shin, Jeong Eun; Park, Kook In

doi:10.1016/j.trsl.2016.12.010

Cited by 18 publications

(24 citation statements)

References 62 publications

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“…Human GDNF cDNA was cloned by PCR and inserted into the CAG-pShuttle plasmid. Adenoviral particles were generated using the AdEasy Adenoviral Vector System (Stratagene, La Jolla, CA) [31]. hNSPCs were transduced with a GDNF-encoding adenoviral vector or a null-encoding adenoviral vector at a multiplicity of infection of 40.…”

Section: Construction and Transduction Of Adenoviral Vectorsmentioning

confidence: 99%

“…For immunodepletion, GDNF-CM was pre-incubated with 5 μg/mL anti-GDNF (R&D Systems) or isotype-matched IgG antibody (R&D Systems) for 30 min at 37℃. After 24 h, neurite lengths were measured as described previously [31]. Each data point represents the average of the data of three independent replicates.…”

Section: Gdnf-hnspcs Enhance Therapeutic Efficiency In Rat With Scimentioning

confidence: 99%

“…Adenovirus-infected cells were plated into 8-well chamber slides (Nunc, Roskilde, Denmark) coated with 10 mg/mL poly-L-lysine (PLL; Sigma) at a density of 8×10 4 cells/well, and differentiated for 7 days in DMEM/F12 containing 1% P/S and 1% N-2 Supplement. Immunocytochemical analysis of cultured cells was performed as described previously [18,31,34]. For immunohistochemical analysis, animals were deeply anesthetized and perfused transcardially with PIPES buffer (pH 7.4; Sigma) containing 4% paraformaldehyde at 9 weeks post-transplantation.…”

Section: Histological Assessment and Stereological Quantificationmentioning

confidence: 99%

“…The T 8~T10 segment of the spinal cord was separated and embedded in O.C.T compound medium (Sakura Finetek, Torrance, CA). Coronal or sagittal cryostat sections of the cord (16 μm thick) were stained with the antibodies listed in Table 1, as described previously [18,31]. For the evaluation of the differentiation patterns of engrafted GDNF-and Mock-hNSPCs in the injured spinal cord, one series of parasagittal sections of the spinal cord of rats injected with Mock-or GDNF-hNSPCs (n=6 per group), each separated by 80 μm, were analyzed with immunohistochemistry.…”

Section: Histological Assessment and Stereological Quantificationmentioning

confidence: 99%

“…Human fetal brain tissue was obtained from a cadaver at 13 weeks of gestation with full parental consent and the approval of the Research Ethics Committee of Yonsei University College of Medicine, Seoul, Korea (Permit No. 4-2003-0078) [18,31]. All procedures conformed with the guidelines of the National Institutes of Health and the Korean Government.…”

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confidence: 99%

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Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury

et al. 2019

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Spinal cord injury (SCI) is a devastating neurological disorder that leads to motor, sensory, or autonomic dysfunction, and is associated with high rates of mortality and complications including bladder infection, renal failure, cardiovascular disease, and respiratory dysfunctions. There are approximately 180,000 new cases of SCI worldwide per year caused by traffic accidents, sports injuries, and other traumatic events according to an epidemiologic study

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Section: Construction and Transduction Of Adenoviral Vectorsmentioning

confidence: 99%

Section: Gdnf-hnspcs Enhance Therapeutic Efficiency In Rat With Scimentioning

confidence: 99%

Section: Histological Assessment and Stereological Quantificationmentioning

confidence: 99%

Section: Histological Assessment and Stereological Quantificationmentioning

confidence: 99%

mentioning

confidence: 99%

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Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury

et al. 2019

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Trilobatin promotes angiogenesis after cerebral ischemia–reperfusion injury via SIRT7/VEGFA signaling pathway in rats

Huang

Luo

et al. 2022

Phytotherapy Research

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Angiogenesis plays a pivotal role in the recovery of neurological function after ischemia stroke. Herein, we investigated the effect of trilobatin (TLB) on angiogenesis after cerebral ischemia–reperfusion injury (CIRI). The effect of TLB on angiogenesis after CIRI were investigated in mouse brain microvascular endothelium bEnd.3 cells and middle cerebral artery occlusion (MCAO)‐induced CIRI rat model. The cell proliferation and angiogenesis were observed using immunofluorescence staining. The cell cycle, expressions of cell cycle‐related proteins and SIRT 1–7 were determined by flow cytometry and western blot, respectively. The binding affinity of TLB with SIRT7 was predicted by molecular docking. The results showed that TLB concentration‐dependently promoted bEnd.3 cell proportion in the S‐phase. TLB significantly increased the protein expressions of SIRT6, SIRT7, and VEGFA, but not affected SIRT1‐SIRT5 protein expressions. Moreover, TLB not only dramatically alleviated neurological impairment after CIRI, but also enhanced post‐stroke neovascularization and newly formed functional vessels in cerebral ischemic penumbra. Furthermore, TLB up‐regulated the protein expressions of CDK4, cyclin D1, VEGFA and its receptor VEGFR‐2. Intriguingly, TLB not only directly bound to SIRT7, but also increased SIRT7 expression at day 28. Our findings reveal that TLB promotes cerebral microvascular endothelial cells proliferation, and facilitates angiogenesis after CIRI via mediating SIRT7/VEGFA signaling pathway in rats. Therefore, TLB might be a novel restorative agent to rescue ischemia stroke.

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Advanced nanotherapies to promote neuroregeneration in the injured newborn brain

Arteaga

Mikrogeorgiou

Kannan

et al. 2019

Advanced Drug Delivery Reviews

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Neonatal brain injury affects thousands of babies each year and may lead to long-term and permanent physical and neurological problems. Currently, therapeutic hypothermia is standard clinical care for term newborns with moderate to severe neonatal encephalopathy. Nevertheless, it is not completely protective, and additional strategies to restore and promote regeneration are urgently needed. One way to ensure recovery following injury to the immature brain is to augment endogenous regenerative pathways. However, novel strategies such as stem cell therapy, gene therapies and nanotechnology have not been adequately explored in this unique age group. In this perspective review, we describe current efforts that promote neuroprotection and potential targets that are unique to the developing brain, which can be leveraged to facilitate neuroregeneration.

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Neurogenin-2 –transduced human neural progenitor cells attenuate neonatal hypoxic-ischemic brain injury

Cited by 18 publications

References 62 publications

Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury

Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury

Trilobatin promotes angiogenesis after cerebral ischemia–reperfusion injury via SIRT7/VEGFA signaling pathway in rats

Advanced nanotherapies to promote neuroregeneration in the injured newborn brain

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