Neurogenic Potential of Implanted Neurospheres Is Regulated by Optogenetic Stimulation of Hippocampal Astrocytes Ex Vivo

Моргун, А. В.; Осипова, Е. Д.; Boitsova, Elizaveta B.; Shuvaev, Аnton N.; Malinovskaya, N. A.; Mosyagina, A. I.; Салмина, А. Б.

doi:10.1007/s10517-021-05135-1

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…Their contribution to the barrier functionality has been described in details elsewhere. 21 , 22 , 27 , 28 …”

Section: Complexity Of the Neurovascular Unit And Key Brain Tissue Ba...mentioning

confidence: 99%

“…All key events within the niche—maintaining the pool of NSCs, their recruitment and proliferation, maturation of NPCs, migration and differentiation of neurobalsts—are driven by the changes in the tissue stiffness and local microenvironment which is provided by the secretory activity of resident cells or by the permeability of the BBB (either in SGZ or SVZ), BCSFC and VB (in SVZ). 28 , 35 , 235 , 236 …”

Section: Development Of a Changeable Brain: 4d Neurogenic Niche In Vi...mentioning

confidence: 99%

“…Even simple static models are still useful for studying intercellular communications in the brain tissue. 17 , 28 , 219 , 263 – 265 Application of microfluidic systems opens new opportunities for the establishment of changeable microenvironment, reconstruction of brain fluids flow and cells behaviors that are controlled by fluids movement and their chemical composition (e.g. fluidic shear stress enabling BMECs to develop and to function with higher efficacy), achievement of better barrier structural integrity as well as continuous monitoring of brain cells metabolism and performing the high throughput high content imaging and analysis.…”

Section: Development Of a Changeable Brain: 4d Neurogenic Niche In Vi...mentioning

confidence: 99%

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Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Salmina,

Alexandrova,

Averchuk

et al. 2024

J Tissue Eng

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In vitro modeling of brain tissue is a promising but not yet resolved problem in modern neurobiology and neuropharmacology. Complexity of the brain structure and diversity of cell-to-cell communication in (patho)physiological conditions make this task almost unachievable. However, establishment of novel in vitro brain models would ultimately lead to better understanding of development-associated or experience-driven brain plasticity, designing efficient approaches to restore aberrant brain functioning. The main goal of this review is to summarize the available data on methodological approaches that are currently in use, and to identify the most prospective trends in development of neurovascular unit, blood-brain barrier, blood-cerebrospinal fluid barrier, and neurogenic niche in vitro models. The manuscript focuses on the regulation of adult neurogenesis, cerebral microcirculation and fluids dynamics that should be reproduced in the in vitro 4D models to mimic brain development and its alterations in brain pathology. We discuss approaches that are critical for studying brain plasticity, deciphering the individual person-specific trajectory of brain development and aging, and testing new drug candidates in the in vitro models.

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“…Their contribution to the barrier functionality has been described in details elsewhere. 21 , 22 , 27 , 28 …”

Section: Complexity Of the Neurovascular Unit And Key Brain Tissue Ba...mentioning

confidence: 99%

Section: Development Of a Changeable Brain: 4d Neurogenic Niche In Vi...mentioning

confidence: 99%

Section: Development Of a Changeable Brain: 4d Neurogenic Niche In Vi...mentioning

confidence: 99%

See 1 more Smart Citation

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Salmina,

Alexandrova,

Averchuk

et al. 2024

J Tissue Eng

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“…Hence, the expression of light-activated molecules in NSKs under the GFAP promoter can regulate their activity and, accordingly, regulate neurogenesis. For example, the optogenetic activation of ChR2 in astrocytes within an artificial neurogenic niche model has been demonstrated to stimulate neurogenesis [165]. However, it is important to note that there is conflicting evidence suggesting that astrocytes that do not express GFAP and vimentin (GFAP−/−Vim−/−) may have an inhibitory effect on neurogenesis and neuronal differentiation.…”

Section: Application Of Optogenetic Approaches To Stimulate Neurogene...mentioning

confidence: 99%

Optogenetics in Alzheimer’s Disease: Focus on Astrocytes

Mitroshina,

Kalinina,

Vedunova

2023

Antioxidants

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Alzheimer’s disease (AD) is the most common form of dementia, resulting in disability and mortality. The global incidence of AD is consistently surging. Although numerous therapeutic agents with promising potential have been developed, none have successfully treated AD to date. Consequently, the pursuit of novel methodologies to address neurodegenerative processes in AD remains a paramount endeavor. A particularly promising avenue in this search is optogenetics, enabling the manipulation of neuronal activity. In recent years, research attention has pivoted from neurons to glial cells. This review aims to consider the potential of the optogenetic correction of astrocyte metabolism as a promising strategy for correcting AD-related disorders. The initial segment of the review centers on the role of astrocytes in the genesis of neurodegeneration. Astrocytes have been implicated in several pathological processes associated with AD, encompassing the clearance of β-amyloid, neuroinflammation, excitotoxicity, oxidative stress, and lipid metabolism (along with a critical role in apolipoprotein E function). The effect of astrocyte–neuronal interactions will also be scrutinized. Furthermore, the review delves into a number of studies indicating that changes in cellular calcium (Ca2+) signaling are one of the causes of neurodegeneration. The review’s latter section presents insights into the application of various optogenetic tools to manipulate astrocytic function as a means to counteract neurodegenerative changes.

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“…Less is known about the application of optogenetics for controlling astroglia-driven regulation of adult neurogenesis. We have demonstrated before that optogenetic stimulation of niche astrocytes expressing channelrhodopsin-2 under the GFAP promoter was efficient in activating the neurogenic potential of NSCs/NPCs in the in vitro neurogenic niche model or in implanted intrahippocampal neurospheres ex vivo in experimental Alzheimer's disease [184,185]. Photostimulation of iPSC-derived ChR2-expressing astrocytes co-cultured with iPSC-derived neurons results in effective bidirectional signaling, which is important for supporting the maturation of neurons and the establishment of a functional synaptic network, even though the transcriptomic analysis confirms that iPSCsoriginated astrocytes are relatively immature compared to adult cortical astrocytes [186].…”

Section: Optogenetic Targeting Of Gfap + Cells In the Neurogenic Niche: Established And Prospective Approaches To Cells Activation And Simentioning

confidence: 99%

Novel Approaches Used to Examine and Control Neurogenesis in Parkinson′s Disease

Салмина

Kapkaeva

Ветчинова

et al. 2021

IJMS

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Neurogenesis is a key mechanism of brain development and plasticity, which is impaired in chronic neurodegeneration, including Parkinson’s disease. The accumulation of aberrant α-synuclein is one of the features of PD. Being secreted, this protein produces a prominent neurotoxic effect, alters synaptic plasticity, deregulates intercellular communication, and supports the development of neuroinflammation, thereby providing propagation of pathological events leading to the establishment of a PD-specific phenotype. Multidirectional and ambiguous effects of α-synuclein on adult neurogenesis suggest that impaired neurogenesis should be considered as a target for the prevention of cell loss and restoration of neurological functions. Thus, stimulation of endogenous neurogenesis or cell-replacement therapy with stem cell-derived differentiated neurons raises new hopes for the development of effective and safe technologies for treating PD neurodegeneration. Given the rapid development of optogenetics, it is not surprising that this method has already been repeatedly tested in manipulating neurogenesis in vivo and in vitro via targeting stem or progenitor cells. However, niche astrocytes could also serve as promising candidates for controlling neuronal differentiation and improving the functional integration of newly formed neurons within the brain tissue. In this review, we mainly focus on current approaches to assess neurogenesis and prospects in the application of optogenetic protocols to restore the neurogenesis in Parkinson’s disease.

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Neurogenic Potential of Implanted Neurospheres Is Regulated by Optogenetic Stimulation of Hippocampal Astrocytes Ex Vivo

Cited by 4 publications

References 15 publications

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Optogenetics in Alzheimer’s Disease: Focus on Astrocytes

Novel Approaches Used to Examine and Control Neurogenesis in Parkinson′s Disease

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