Neurogenic differentiation of murine and human adipose-derived stromal cells

Safford, Kristine M.; Hicok, Kevin C.; Safford, Shawn D.; Halvorsen, Yuan Di C.; Wilkison, William O.; Gimble, Jeffrey M.; Rice, Henry E.

doi:10.1016/s0006-291x(02)00469-2

Cited by 697 publications

(447 citation statements)

References 39 publications

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“…Recent studies have confirmed the existence of an abundant and easily accessible source of multipotent stem cells in subcutaneous or intraarticular adipose tissue (13)(14)(15)(16)(17)(18)(19)(20)(21). Cells obtained from adipose tissue have been termed processed lipoaspirate cells, adiposederived adult stromal/stem cells (ADAS cells), or simply adipose-derived stem cells.…”

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confidence: 99%

“…The goal of the present study was to investigate the effects of TGF␤1, TGF␤3, IGF-1, BMP-6, and dexamethasone on ADAS cell chondrogenesis; therefore, only markers associated with understanding of the process of chondrogenesis were studied. Other markers that have been associated with ADAS cells, such as adipogenic and neuronal markers (16)(17)(18)21,32), were specifically excluded in this analysis. Passage-4 ADAS cells were cultured in vitro for 7 days in a 3-D alginate culture system under various combinations of the abovementioned growth factors.…”

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confidence: 99%

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Potent induction of chondrocytic differentiation of human adipose‐derived adult stem cells by bone morphogenetic protein 6

Estes

Guilak

2006

Arthritis & Rheumatism

269

212

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Objective. Recent studies have identified an abundant source of multipotent progenitor cells in subcutaneous human adipose tissue, termed human adiposederived adult stem cells (ADAS cells). In response to specific media formulations, including transforming growth factor ␤1 (TGF␤1), these cells exhibit significant ability to differentiate into a chondrocyte-like phenotype, expressing cartilage-specific genes and proteins such as aggrecan and type II collagen. However, the influence of other growth factors on the chondrogenic differentiation of ADAS cells is not fully understood. This study was undertaken to investigate the effects of TGF␤1, TGF␤3, insulin-like growth factor 1, bone morphogenetic protein 6 (BMP-6), and dexamethasone, in various combinations, on the chondrogenic potential of ADAS cells in alginate beads.Methods. The chondrogenic response of alginateencapsulated ADAS cells was measured by quantitative polymerase chain reaction, 3 H-proline and 35 S-sulfate incorporation, and immunolabeling for specific extracellular matrix components.Results. Significant differences in chondrogenesis were observed under the different culture conditions for all outcomes measured. Most notably, BMP-6 upregulated AGC1 and COL2A1 expression by an average of 205-fold and 38-fold, respectively, over day-0 controls, while down-regulating COL10A1 expression by ϳ2-fold.Conclusion. These findings suggest that BMP-6 is a potent inducer of chondrogenesis in ADAS cells, in contrast to mesenchymal stem cells, which exhibit increased expression of type X collagen and a hypertrophic phenotype in response to BMP-6. Combinations of growth factors containing BMP-6 may provide a novel means of regulating the differentiation of ADAS cells for applications in the tissue-engineered repair or regeneration of articular cartilage.

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confidence: 99%

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confidence: 99%

Potent induction of chondrocytic differentiation of human adipose‐derived adult stem cells by bone morphogenetic protein 6

Estes

Guilak

2006

Arthritis & Rheumatism

269

212

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“…These adipose tissue-derived stem cells (ADSCs) have an osteogenic capacity both in vitro (Halvorsen et al, 2001) and in vivo. Similarly, ADSCs can be induced to differentiate in vitro into various other cell lines, including chondrogenic (Erickson et al, 2002;Huang et al, 2004), adipogenic, hepatic (Seo et al, 2005) and neurogenic lineages (Safford et al, 2002;Ashjian et al, 2003). Mizuno et al (2002) showed that culture of ADSCs in myogenic conditions led to expression of muscle-related genes.…”

Section: Bone Marrow-derived Stromal Stem Cellsmentioning

confidence: 99%

Updates on stem cells and their applications in regenerative medicine

Bajada

Mazakova

Richardson

et al. 2008

J Tissue Eng Regen Med

305

188

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“…Importantly, new evidence supports the differentiation of AS cells across lineages so that easily accessible stem cells could be used in the therapy of degenerative diseases of the CNS [Ross and Verfaillie, 2008]. For example, neurons and glia can be produced from skin derived precursor cells isolated from human scalp and foreskin [Toma et al, 2005], marrow stromal stem cells [Cho et al, 2005;Togel and Westenfelder, 2007], umbilical cord stem cells [SanchezRamos et al, 2001;Low et al, 2008], and adipose tissue-derived stromal cells [Safford et al, 2002;Schaffler and Buchler, 2007]. Given their accessibility, abundance, ethical justification, and plasticity, these non-NS cells represent viable alternatives for discerning etiopathologies and pharmaco-screening.…”

Section: Human Adult Stem Cellsmentioning

confidence: 99%

Human stem cells for modeling neurological disorders: Accelerating the drug discovery pipeline

Crook

Kobayashi

2008

J of Cellular Biochemistry

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The availability of human stem cells heralds a new era for modeling normal and pathologic tissues and developing therapeutics. For example, the in vitro recapitulation of normal and aberrant neurogenesis holds significant promise as a tool for de novo modeling of neurodevelopmental and neurodegenerative diseases. Translational applications include deciphering brain development, function, pathologies, traditional medications, and drug discovery for novel pharmacotherapeutics. For the latter, human stem cell-based assays represent a physiologically relevant and high-throughput means to assess toxicity and other undesirable effects early in the drug development pipeline, avoiding latestage attrition whilst expediting proof-of-concept of genuine drug candidates. Here we consider the potential of human embryonic, adult, and induced pluripotent stem cells for studying neurological disorders and preclinical drug development.

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Neurogenic differentiation of murine and human adipose-derived stromal cells

Cited by 697 publications

References 39 publications

Potent induction of chondrocytic differentiation of human adipose‐derived adult stem cells by bone morphogenetic protein 6

Potent induction of chondrocytic differentiation of human adipose‐derived adult stem cells by bone morphogenetic protein 6

Updates on stem cells and their applications in regenerative medicine

Human stem cells for modeling neurological disorders: Accelerating the drug discovery pipeline

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