Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract

Eriani, Kartini; Suryani, Irma; Azhar, Al; Nursanti, Risa; Ichsan, Ichsan; Boediono, Arief

doi:10.15294/biosaintifika.v10i2.15051

Cited by 5 publications

(8 citation statements)

References 10 publications

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“…The effect of this drug on both blood glucose and pancreatic cell recovery is lower compared to those caused by flamboyant flower extracts. Results obtained in this study, together with those previoulsy presented about the effect of D. regia extract on the proliferation and differentiation of bone originated stem cells (Eriani et al 2018) has showed possible use of the plant extract in diabetes management and therapy, an important contibution in the way to study the antidiabetic exploration of the plant extract for the development of antidiabetic formulation.…”

Section: Degeneration and Necrosis Of Pancreatic β-Cellsupporting

confidence: 68%

“…Previous studies have shown the potential of flamboyant flower extract to induce differentiation of mesenchymal stem cells (MSC) into nerve cells (Eriani et al 2018) and osteoblasts (Eriani et al 2020) in vitro. The MSCs, that are almost present in all tissues in the body such as bone marrow cells, adipose tissue, muscle, heart, and lung (Wei et al 2013;Williams & Hare 2011) have potential as immunomodulators and tissue regeneration.…”

Section: Degeneration and Necrosis Of Pancreatic β-Cellmentioning

confidence: 99%

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Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers

et al. 2021

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Providing scientific evidences for the medicinal benefits and possible toxic effects of the flamboyant flowers (Delonix regia (Bojer ex Hook.) Raf.) are very important to implementing the medicinal plant in this modern era. This study aimed to investigate antidiabetic potential of methanol extract of flamboyant flowers using a completely random design. Twenty-four male rats were randomly divided into 6 treatment groups with 4 replications: healthy rats (normal control, P1), diabetic rats treated with flamboyant flower extract of 0 (negative control, P2), 100 (P3), 200 (P4), and 400 mg/kg BW (P5), and rats which were induced by 0.45 mg/kg BW of Glibenclamide (positive control, P6). Diabetic condition was achieved by a single injection of alloxan 150 mg/kg BW. Treatments were given once a day for 14 days. On day 0, 3 and 18 blood samples were withdrawn from rats’ orbital vein for glucose measurement. All rats were sacrificed for liver, gastrocnemius muscles and pancreatic tissues collection. The liver and gastrocnemius muscle were subjected for glycogen measurement whereas pancreatic tissues were processed for histological examinations. Data was analyzed by ANOVA and followed by Duncan test. The results showed that flamboyant flowers extracts significantly (p<0.05) reduced blood glucose as well as degeneration and necrosis of pancreatic β cells. Optimal dose to decrease blood glucose and pancreatic cell degeneration was 200 mg/kg BW; whereas optimum dose to decrease pancreatic cell necrosis was 400 mg/kg BW. In conclusion, flamboyant flower extract can reduce blood glucose in rats. This is the first that shows antidiabetic potential of local Flamboyant flower extracts along its toxicity effect to pancreatic tissues. These information could become a basic consideration for the use of the plant extracts as a candidate to cure patients with diabetic problems.

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Section: Degeneration and Necrosis Of Pancreatic β-Cellsupporting

confidence: 68%

Section: Degeneration and Necrosis Of Pancreatic β-Cellmentioning

confidence: 99%

Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers

et al. 2021

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“…The control band was not clear, perhaps due to a pipetting error. The size of the DNA band of the primer β-actin was about 443bp as expected, and the primer β-tubulin 3 was about 700bp, while according to Wang et al, 11 the size of targeted β-tubulin 3 was 55bp. This size difference was probably because the primer design was based on Rattus novergicus, the brown rat, while in this study we used M. musculus.…”

Section: Number Of Differentiated Mesenchymal Stem Cells Into Nerve L...supporting

confidence: 64%

“…The mixture was then sterilized using microfiltration with a diameter of 0.22 μm. 11 Housing and husbandry of animals The male M. musculus mice of the strain BALB/c (eight weeks of age) were acclimated and fed ad libitum with standardized feed for one week under laboratory conditions with a 12 h light-dark cycle, 60% of humidity with 23 o C. To minimize the number of animals, six mice were used with assumption that the number of MSCs from one mouse was enough for one set of repetitions (i.e., with four repetitions in this study, two other mice were prepared for any unexpected event such as if the MSCs did not grow).…”

Section: Preparation Of Dulbecco's Modified Eagle's Medium (Mdmem)mentioning

confidence: 99%

The differentiation of mesenchymal bone marrow stem cells into nerve cells induced by Chromolaena odorata extracts

et al. 2022

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Background: Mesenchymal stem cells (MSCs) can differentiate into nerve cells with an induction from chemical compounds in medium culture. Chromolaena odorata contains active compounds, such as alkaloids and flavonoids, that can initiate the transformation of MSCs into nerve cells. The aim of this study was to determine the potential of methanol extracted C. odorata leaf to induce the differentiation of bone marrow MSCs into nerve cells. Methods: A serial concentration of C. odorata leaf extract (0.7–1.0 mg/mL) with two replications was used. The parameters measured were the number of differentiated MSCs into nerve cells (statistically analyzed using ANOVA) and cell confirmation using reverse transcription polymerase chain reaction (RT-PCR). Results: The results showed that the C. odorata extract had a significant effect on the number MSCs differentiating into nerve cells (p < 0.05) on the doses of 0.8 mg/ml with 22.6%. Molecular assay with RT-PCR confirmed the presence of the nerve cell gene in all of the samples. Conclusions: In conclusion, this study showed the potential application of C. odorata leaf extract in stem cell therapy for patients experiencing neurodegeneration by inducing the differentiation of MSCs into nerve cells.

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“…The cervical dislocation was carried out in four three-month-old male mice of the Balb-C strain. The method of isolation and primary culture of MSC bone marrow in this study were based on the protocol of Eriani, et al, (2018).…”

Section: Isolation and Primary Culture Of Bone Marrow Mscmentioning

confidence: 99%

The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro

2022

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Physalis minimahas been used as herbal medicine because it is believed by the community to cure neurodegenerative and cardiovascular diseases. This study aims to determine the effectiveness of P.minima extract in increasing the proliferation of mesenchymal stem cells (MSC) from mouse (Musmusculus) bone marrow (BM). BM from the femur and tibia were isolated using a flushing method. BM-MSC primary culture was conducted in mesenPROÒ medium at 37ᵒC in a 5% CO2 incubator until it reached a 70% confluence.BM-MSCs were sub-cultured overnight in Dulbecco's Modified Eagle's Media (mDMEM). The mDMEM was replaced with a treatment medium on the second day of subculture. The treatment medium was changed every three days and evaluated under an inverted microscope by counting the number of cells at the beginning and the end of the incubation period. The proliferation rate is expressed as PDT, which was statistically analyzed using ANOVA at a significance level of 0.05% and followed-up with Duncan's test. Statistically, P. minima leaf extract could significantly reduce the PDT value. The optimum dose of P. minima leaf extract that can increase the proliferation of BM-MSC was 0.8 mg/ml. It is concluded that P. minima leaf extract was effective as an inducer of BM-MSC proliferation. The data obtained is the preliminary data on the use of P.minimaextracts in stem cell-based therapy. The results of this study provide important information in scientifically proving the potential of P. minima extract on stem cell proliferation.

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Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract

Cited by 5 publications

References 10 publications

Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers

Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers

The differentiation of mesenchymal bone marrow stem cells into nerve cells induced by Chromolaena odorata extracts

The Effect of Ciplukan (Physalis minima) Leaf Extract on Mesenchymal Stem Cell Proliferation and Population Doubling Time (PDT) In Vitro

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