Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord

Arulkandarajah, Kalaimakan Hervé; Osterstock, Guillaume; Lafont, Agathe; Corronc, Hervé Le; Escalas, Nathalie; Corsini, Silvia; Bras, Barbara Le; Chenane, Linda; Boeri, Juliette; Czarnecki, Antonny; Mouffle, Christine; Bullier, Erika; Hong, Elim; Soula, Cathy; Legendre, Pascal

doi:10.1016/j.cub.2021.08.019

Cited by 7 publications

(2 citation statements)

References 60 publications

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“…In addition, in our opinion, the growth of satellite glia indicates a high degree of functional activity of neurons, therefore the nature of structural and quantitative changes of glia demonstrates high plasticity of nervous tissue. The issue of discussion today is the study of the intensity of the proliferation processes of the dorsal neuroepithelium of the human spinal cord during the prenatal period [23] and their effect on the density of neural cells in the posterior horns [2,14], as this is the basis for the formation of the NGC [5].…”

Section: Discussionmentioning

confidence: 99%

Neuron-glial relations of the posterior horns of the spinal cord of human fetuses

Prykhodko

Shkolnikov

2022

Rep. of Morph.

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Despite the relatively sufficient study of the structure and functioning of the nervous system, interest in the problem of neuron-glial relationships continues to grow steadily, as this parameter reflects the dynamics of the development of nervous tissue and can be used to assess the quality level of morphological changes. The purpose of the study: to establish the morphogenesis and neuron-glial relationships of the posterior horns of the human spinal cord in the fetal period of ontogenesis. This study was performed on the preparations of 104 human fetuses from 8-9 weeks to 39-40 weeks using anatomical, histological, immunohistochemical and morphometric methods. Statistical processing of the numerical data of the obtained results was carried out using the licensed software package “Statistica 6.1” of the StatSoft company using parametric and non-parametric methods. During the research, it was established that in the fetal period, the greater proliferative activity of the dorsal neuroepithelium is determined at 8-9 weeks: in the cervical segments – 10 % (р<0.05), in the thoracic, lumbar and sacral segments – 9 % (р<0.05). By 39-40 weeks, this indicator gradually becomes smaller: in the cervical and lumbar segments, 4 % of cells (2-3 cells reacted) (p<0.05) and in the thoracic and sacral segments – 3 % (1-2 cells reacted) (p<0.05). It was found that throughout the fetal period there is a tendency to a gradual decrease in the density of neurons and gliocytes. The glial index, on the contrary, up to 39-40 weeks increases, and at the time of birth it is equal to 2.1 in the cervical, thoracic and lumbar segments, and 2.0 in the sacral segments. It was found that at 11-12 weeks, radial glia fibers form mesh structures within the neuronal complexes, which coincides with the beginning of the formation of neuron-glial complexes of the posterior horns. At 17-18 weeks, the fibers of radial glia keep the radial direction only in the middle part of the posterior horns. At 34-35 weeks, vimentin expression was determined to be relatively moderate in the remnants of radial glia near the dorsal neuroepithelium and focal expression of vimentin around vessels within the posterior horns. Expression of vimentin in the neuroepithelium of fetuses of 39-40 weeks was absent. In this age period, the neuroepithelium is structured from ependymocytes and radial glia cells are absent, as there is a relatively strong expression of S-100 in the neuroepithelium. Relatively strong expression of synaptophysin occurred in the posterior horns of 8-9 week fetuses. This age period is the beginning of the establishment of synaptic connections.

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Section: Discussionmentioning

confidence: 99%

Neuron-glial relations of the posterior horns of the spinal cord of human fetuses

Prykhodko

Shkolnikov

2022

Rep. of Morph.

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“…Especially in excitable tissues, Na þ and K þ ion-mediated action potentials (ranging from À10 to À90 mV) can trigger the proliferation and differentiation of different types of cells through intercellular communication. [17,18] Electric fields play a crucial role in cell physiology, regulating most cellular processes in nearly all cell types. Therefore, generating and modulating electrical signals shows great potential for rapid recovery of injured tissue.…”

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confidence: 99%

Piezoelectric Nanomaterial‐Mediated Physical Signals Regulate Cell Differentiation for Regenerative Medicine

Li,

Pan,

Wang

et al. 2024

Small Science

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Tissue damage often causes considerable suffering to patients due to slow recovery and poor prognosis. The use of electroactive materials to deliver biophysical signals plays a key role in regulating tissue regeneration processes. Among these materials, piezoelectric materials have unique electromechanical conversion capabilities, making them suitable for use as cell scaffolds. They can deform and emit electrical signals in response to external stimuli, thereby regulating cell proliferation and differentiation. In this review, recent advances are presented in piezoelectric materials as physical signaling mediators that regulate cell differentiation. The basic mechanisms, classification of these materials, and their different applications in tissue regeneration are described. Finally, a comprehensive discussion of current challenges and prospects in the field is provided. Together, existing experimental results basically show that piezoelectric materials can improve the process and effect of tissue repair, providing new technical options for the development of tissue engineering in the future.

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Patch-seq: Advances and Biological Applications

Shao,

Zhang,

et al. 2023

Cell Mol Neurobiol

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Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord

Cited by 7 publications

References 60 publications

Neuron-glial relations of the posterior horns of the spinal cord of human fetuses

Neuron-glial relations of the posterior horns of the spinal cord of human fetuses

Piezoelectric Nanomaterial‐Mediated Physical Signals Regulate Cell Differentiation for Regenerative Medicine

Patch-seq: Advances and Biological Applications

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