Neuroendocrine markers are expressed in human mammary glands

Grönberg, Malin; Amini, Rose‐Marie; Stridsberg, Mats; Janson, Eva Tiensuu; Saras, Jan

doi:10.1016/j.regpep.2009.12.011

Cited by 23 publications

(11 citation statements)

References 42 publications

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“…This widely expressed in the body a 23-amino acid peptide is encoded on preproghrelin gene region. Obestatin has been found in the small intestine, colon, spleen, cerebral cortex, skeletal muscle, adipose tissue, lung, liver and many other tissues, but the major production of obestatin in rats takes place in the fundic region of gastric mucosa (Zhang et al, 2005;Zhao et al, 2008;Grönberg et al, 2010;Moretti et al, 2014). In the pancreas, obestatin-immunoreactive cells are mainly present in the peripheral region of the islets and do not co-localize with glucagon, pancreatic polypeptide Y or insulin secreting cells .…”

Section: Introductionmentioning

confidence: 99%

Exogenous obestatin affects pancreatic enzyme secretion in rat through two opposite mechanisms, direct inhibition and vagally-mediated stimulation

Kapica¹,

Puzio²,

Kato³

et al. 2018

J. Anim. Feed Sci.

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Section: Introductionmentioning

confidence: 99%

Exogenous obestatin affects pancreatic enzyme secretion in rat through two opposite mechanisms, direct inhibition and vagally-mediated stimulation

Kapica¹,

Puzio²,

Kato³

et al. 2018

J. Anim. Feed Sci.

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“…Using immunohistochemistry, we have demonstrated the presence of ghrelin and obestatin in normal female and male breast tissue, where the majority of samples expressed the peptides 32 . When comparing the immunohistochemical patterns in our previous studies on FBC 34 , 35 with the present study on MBC, the expression of the peptides seem to be similar.…”

Section: Discussionmentioning

confidence: 97%

Ghrelin expression is associated with a favorable outcome in male breast cancer

Grönberg

Nilsson²,

Markholm

et al. 2018

Sci Rep

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Ghrelin and obestatin are two gastrointestinal peptides, derived from a common precursor. Expression of both peptides have been found in breast cancer tissue and ghrelin has been associated with breast cancer development. Ghrelin expression is associated with longer survival in women diagnosed with invasive and node negative breast cancer. The clinical implications of the peptide expression in male breast cancer are unclear. The aim of this study was to investigate the role and potential clinical value of ghrelin and obestatin in male breast cancer. A tissue microarray of invasive male breast cancer specimens from 197 patients was immunostained with antibodies versus the two peptides. The expression of the peptides was correlated to previously known prognostic factors in breast cancer and to the outcome. No strong correlations were found between ghrelin or obestatin expression and other known prognostic factors. Only ghrelin expression was statistically significantly correlated to breast cancer-specific survival (HR 0.39, 95% CI 0.18–0.83) in univariate analyses and in multivariate models, adjusted for tumor size and node status (HR 0.38, 95% CI 0.17–0.87). HR for obestatin was 0.38 (95% CI 0.11–1.24). Ghrelin is a potential prognostic factor for breast cancer death in male breast cancer. Patients with tumors expressing ghrelin have a 2.5-fold lower risk for breast cancer death than those lacking ghrelin expression. Drugs targeting ghrelin are currently being investigated in clinical studies treating metabolic or nutritional disorders. Ghrelin should be further evaluated in forthcoming studies as a prognostic marker with the aim to be included in decision algorithms.

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“…We are showing the evidence of expression of both SV2 protein and mRNA in non-tumor and transformed breast cell lines; we found SV2 A, B and C isoforms expression in breast cell lines. A recent research indicates that cells of the luminal epithelium of ducts and lobules of human mammary glands express neuroendocrine markers such as vesicular monoamine transporter 2 and chromogranin B, as well as the regulatory peptides obestatin, ghrelin, adrenomedullin and apelin (Gronberg et al, 2010), furthermore neuronal/glial markers (nestin, TUBB3 and GFAP) have been identified in MCF-10A, SKBR3 and MBA-MB-231 cell lines, although they are overexpressed in tumor-cell lines, these breast cancer lines are showing a multi-lineage differentiation potential of breast cancer cell lines to express multiple neuronal/glial lineage-specific markers as well as mammary epithelial and melanocyticspecific markers (Zhang et al, 2010). Furthermore, in the same study ectoderm multi-lineage transdifferentiation was also found in human melanoma (Ul-MeL) and glioblastoma cell lines (U87 and D54).…”

Section: Discussionmentioning

confidence: 99%

Synaptic Vesicle Protein 2 (SV2) Isoforms

Bandala

Miliar-García²,

Mejía-Barradas

et al. 2012

Asian Pacific Journal of Cancer Prevention

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New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to detemine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.

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Neuroendocrine markers are expressed in human mammary glands

Cited by 23 publications

References 42 publications

Exogenous obestatin affects pancreatic enzyme secretion in rat through two opposite mechanisms, direct inhibition and vagally-mediated stimulation

Exogenous obestatin affects pancreatic enzyme secretion in rat through two opposite mechanisms, direct inhibition and vagally-mediated stimulation

Ghrelin expression is associated with a favorable outcome in male breast cancer

Synaptic Vesicle Protein 2 (SV2) Isoforms

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