Neuroendocrine disruption and health effects in Elliptio complanata mussels exposed to aeration lagoons for wastewater treatment

Gagné, François; Blaise, C.; André, C.; Gagnon, Christian; Salazar, Michael

doi:10.1016/j.chemosphere.2006.12.101

Cited by 57 publications

(32 citation statements)

References 41 publications

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“…COX activity was measured as described by Gagné et al 6,13 using the oxidation of 2,7-dichlorofluorescin in the presence of arachidonate, previously described in Fijimoto et al 16,17 Briefly, S15 fraction were mixed with arachidonate, dichlorofluorescin and horseradish peroxidase in a Tris-HCL buffer (pH 8) containing Tween 20. The appearance of fluorescein was measured every 2 min for 20 min at 485 nm (excitation) and 520 nm (emission) with a spectrofluorimeter (Spectra Max M5, Molecular devices).…”

Section: Spawning Biomarkermentioning

confidence: 99%

Decrease in phagocytosis capacity of hemocyte during spawning in Mytilus edulis: a pilot study

Fraser¹,

Rault²,

Roumier³

et al. 2013

J Xenobiotics

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Section: Spawning Biomarkermentioning

confidence: 99%

Decrease in phagocytosis capacity of hemocyte during spawning in Mytilus edulis: a pilot study

Fraser¹,

Rault²,

Roumier³

et al. 2013

J Xenobiotics

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“…Many endocrine-related effects can be significantly associated with oxidative metabolisms and stress endpoints, which are associated with tissue damage (Gagné et al 2007). In the present study, Vg-like proteins and LPO patterns were the opposite in mussels but similar in clams.…”

Section: Mytilus Galloprovincialismentioning

confidence: 48%

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Pereira¹,

Gomes²,

Cardoso³

et al. 2013

Sci. Mar.

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SUMMARY: Estuaries are highly productive ecosystems that have been increasingly affected by anthropogenic activities. In this study, the presence of endocrine disrupting compounds (EDCs) and their relation to oxidative stress were assessed in two bivalve species (mussels Mytilus galloprovincialis and clams Ruditapes decussatus) from the Ria Formosa Lagoon (south Portugal). We applied the alkali-labile phosphate (ALP) assay to determine vitellogenin-like protein levels, and the lipid peroxidation (LPO) method to assess oxidative damage. Mussels and clams exhibited the same pattern of ALP concentrations, with no differences between genders and higher levels in winter. R. decussatus showed higher ALP values than M. galloprovincialis, possibly associated with a stronger responsiveness of clams to EDCs or due to the influence of the reproductive cycle. Mussels exhibited a more evident seasonal effect for LPO, indicating that these organisms are more influenced by seasonal fluctuations in the water column compared to the sediment-associated clams. Both species appear to be under the influence of EDC exposure (which could induce Vg-like protein synthesis) and oxidative stressors (leading to lipid peroxidation) in the Ria Formosa Lagoon, particularly in winter, reflecting the presence of different contaminants in Ria Formosa and environmental changes, as well as complex interactions between them.Keywords: Ria Formosa Lagoon, Mytilus galloprovincialis, Ruditapes decussatus, endocrine disruption, vitellogenin-like proteins, lipid peroxidation. RESUMEN: Variabilidad interespecífica de disruptores endocrinos y estrés oxidatiVo en dos especies de biValVos de la ría formosa (costa sur de portugal). -Los estuarios son ecosistemas altamente productivos afectados por actividades antropogénicas. En este trabajo, la presencia de compuestos disruptores endocrinos (EDCs) y su relación con estrés oxidativo se ha evaluado en los bivalvos Mytilus galloprovincialis y Ruditapes decussatus en la laguna Ría Formosa (Portugal). Se utilizó el ensayo de fosfatos lábil alcalinos (ALP) para determinar los niveles de proteína tipo-vitelogenina y el método de peroxidación lipídica (LPO) para evaluar los daños oxidativos. Ambas especies exhibieron el mismo patrón de concentración de ALP: no hubo diferencias entre sexos pero si niveles más altos en invierno. R. decussatus presentó valores mayores de ALP que M. galloprovincialis posiblemente asociados a una capacidad de respuesta más elevada a los EDCs o al estado del ciclo reproductor. Los mejillones, al no estar asociados al sedimento como las almejas, mostraron variaciones estacionales en las concentraciones de LPO, indicando mayor sensibilidad a fluctuaciones ambientales en la columna de agua. Ambas especies parecen estar influenciadas (principalmente en invierno) por EDCs (posiblemente induciendo la síntesis de proteína tipo-vitelogenina) y factores de estrés oxidativo (activando la peroxidación lipídica); lo que refleja la presencia de diferentes contaminantes en la Ría Formosa y los cambios a...

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“…This suggests that the process of energy metabolism to support gametogenesis contributes to oxidative stress, in conjunction with temperature sensitivity. However, pollution could disrupt reproduction at the neuroendocrine level in mussels [23]. Altered production of vitellogenin-like proteins was associated with increased inflammation as determined by LPO, cyclooxygenase activity at the high dopamine/serotonin ratio.…”

Section: Discussionmentioning

confidence: 99%

Urban Pollution Increases Sensitivity to Temperature Changes in Elliptio complanata Mussels Exposed to Municipal Wastewaters

André¹,

Auclair²,

Gagne³

2017

Environ Pollut Climate Change

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Neuroendocrine disruption and health effects in Elliptio complanata mussels exposed to aeration lagoons for wastewater treatment

Cited by 57 publications

References 41 publications

Decrease in phagocytosis capacity of hemocyte during spawning in Mytilus edulis: a pilot study

Decrease in phagocytosis capacity of hemocyte during spawning in Mytilus edulis: a pilot study

Interspecific variability of endocrine disruption and oxidative stress in two bivalve species from the Ria Formosa Lagoon (south coast of Portugal)

Urban Pollution Increases Sensitivity to Temperature Changes in Elliptio complanata Mussels Exposed to Municipal Wastewaters

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