Neuroendocrine Control of Larval Ecdysis Behavior in<i>Drosophila</i>: Complex Regulation by Partially Redundant Neuropeptides

Clark, Anthony C.; Campo, Marta L. del; Ewer, John

doi:10.1523/jneurosci.4938-03.2004

Cited by 98 publications

(114 citation statements)

References 31 publications

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“…Neuroendocrine events and behavioral defects were carefully examined in these transgenic knockout flies and compared with controls (McNabb et al, 1997;Clark et al, 2004;Kim et al, 2006b). Pupation of animals with ablated thoracic FMRFamide-producing ETHR-A neurons was normal, except pre-ecdysis contractions appeared weaker (Kim et al, 2006b).…”

Section: Roles Of Central Neuropeptides and Eth Receptors In Ecdysismentioning

confidence: 99%

“…Pupation of animals with ablated thoracic FMRFamide-producing ETHR-A neurons was normal, except pre-ecdysis contractions appeared weaker (Kim et al, 2006b). Likewise, ablation of EH-producing neurons caused only subtle alterations in duration of larval and pupal ecdysis sequence, but affected tracheal inflation, adult eclosion and post-eclosion behaviors (McNabb et al, 1997;Clark et al, 2004;Kim et al, 2006b). …”

Section: Roles Of Central Neuropeptides and Eth Receptors In Ecdysismentioning

confidence: 99%

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Complex steroid–peptide–receptor cascade controls insect ecdysis

Žitňan

Kim

Žitňanová

et al. 2007

General and Comparative Endocrinology

155

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SUMMARYInsect ecdysis sequence is composed of pre-ecdysis, ecdysis and post-ecdysis behaviors controlled by a complex cascade of peptide hormones from endocrine Inka cells and neuropeptides in the central nervous system (CNS). Inka cells produce pre-ecdysis and ecdysis triggering hormones (ETH) which activate the ecdysis sequence through receptor-mediated actions on specific neurons in the CNS. Multiple experimental approaches have been used to determine mechanisms of ETH expression and release from Inka cells and its action on the CNS of moths and flies. During the preparatory phase 1-2 days prior to ecdysis, high ecdysteroid levels induce expression of ETH receptors in the CNS and increased ETH production in Inka cells, which coincides with expression of nuclear ecdysone receptor (EcR) and transcription factor cryptocephal (CRC). However, high ecdysteroid levels prevent ETH release from Inka cells. Acquisition of Inka cell competence to release ETH requires decline of ecdysteroid levels and β-FTZ-F1 expression few hours prior to ecdysis. The behavioral phase is initiated by ETH secretion into the hemolymph, which is controlled by two brain neuropeptides -corazonin and eclosion hormone (EH). Corazonin acts on its receptor in Inka cells to elicit low level ETH secretion and initiation of pre-ecdysis, while EH induces cGMP-mediated ETH depletion and consequent activation of ecdysis. The activation of both behaviors is accomplished by ETH action on central neurons expressing ETH receptors A and B (ETHR-A and B). These neurons produce numerous excitatory or inhibitory neuropeptides which initiate or terminate different phases of the ecdysis sequence. Our data indicate that insect ecdysis is a very complex process characterized by two principal steps: 1) Ecdysteroid-induced expression of receptors and transcription factors in the CNS and Inka cells. 2) Release and interaction of Inka cell peptide hormones and multiple central neuropeptides to control consecutive phases of the ecdysis sequence.

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Section: Roles Of Central Neuropeptides and Eth Receptors In Ecdysismentioning

confidence: 99%

Section: Roles Of Central Neuropeptides and Eth Receptors In Ecdysismentioning

confidence: 99%

Complex steroid–peptide–receptor cascade controls insect ecdysis

Žitňan

Kim

Žitňanová

et al. 2007

General and Comparative Endocrinology

155

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“…The elevation of cGMP by EH appears to be a key step in the induction of massive ETH release, leading its depletion in Inka cells (10). In Drosophila, corazonin appears not to be involved in ETH release, but elevation of cGMP in Inka cells before ecdysis initiation lends credence to a role for EH in ETH release in this organism as well (11).…”

Section: Discussionmentioning

confidence: 96%

“…The release of ETH from Inka cells is mediated by brain neuropeptides, which mobilize cytoplasmic calcium and cGMP (11,24). In Manduca, corazonin mediates low rates of ETH release during pre-ecdysis before EH-induced cGMP elevation.…”

Section: Discussionmentioning

confidence: 99%

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Receptor guanylyl cyclases in Inka cells targeted by eclosion hormone

Chang

Yang

Adams

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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A signature of eclosion hormone (EH) action in insect ecdysis is elevation of cGMP in Inka cells, leading to massive release of ecdysis triggering hormone (ETH) and ecdysis initiation. Although this aspect of EH-induced signal transduction is well known, the receptor mediating this process has not been identified. Here, we describe a receptor guanylyl cyclase BdmGC-1 and its isoform BdmGC-1B in the Oriental fruit fly Bactrocera dorsalis that are activated by EH. The B form exhibits the conserved domains and putative N-glycosylation sites found in BdmGC-1, but possesses an additional 46-amino acid insertion in the extracellular domain and lacks the C-terminal tail of BdmGC-1. Combined immunolabeling and in situ hybridization reveal that BdmGC-1 is expressed in Inka cells. Heterologous expression of BdmGC-1 in HEK cells leads to robust increases in cGMP following exposure to low picomolar concentrations of EH. The B-isoform responds only to higher EH concentrations, suggesting different physiological roles of these cyclases. We propose that BdmGC-1 and BdmGC-1〉 are high-and low-affinity EH receptors, respectively.

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A cyclic nucleotide‐gated channel in the brain regulates olfactory modulation through neuropeptide F in fruit fly Drosophila melanogaster

Lee

Jones

Kim

2019

Arch Insect Biochem Physiol

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Olfactory sensing and its modulation are important for the insects in recognizing diverse odors from the environment and in making correct decisions to survive. Identifying new genes involved in olfactory modulation and unveiling their mechanisms may lead us to understand decision making processes in the central nervous system. Here, we report a novel olfactory function of the cyclic nucleotide-gated (CNG) channel CG42260 in modulating ab3A olfactory sensory neurons, which specifically respond to food-derived odors in fruit fly Drosophila melanogaster. We found that two independent CG42260 mutants show reduced responses in the ab3A neurons. Unlike mammalian CNGs, CG42260 is not expressed in the odorant sensory neurons but broadly in the central nervous system including neuropeptide-producing cells. By using molecular genetic tools, we identified CG42260 expression in one pair of neuropeptide F (NPF) positive L1-l cells known to modulate food odor responsiveness. Knockdown of CG42260 in the NPF neurons reduced production of NPF in Ll-1 cells, which in turn, led to reduction of neuronal responses of the ab3A neurons. Our findings show the novel biological function of CG42260 in modulating olfactory responses to food odor through NPF. K E Y W O R D S

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Neuroendocrine Control of Larval Ecdysis Behavior inDrosophila: Complex Regulation by Partially Redundant Neuropeptides

Cited by 98 publications

References 31 publications

Complex steroid–peptide–receptor cascade controls insect ecdysis

Complex steroid–peptide–receptor cascade controls insect ecdysis

Receptor guanylyl cyclases in Inka cells targeted by eclosion hormone

A cyclic nucleotide‐gated channel in the brain regulates olfactory modulation through neuropeptide F in fruit fly Drosophila melanogaster

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