Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic <i>orco</i> mutation in honey bees

Chen, Zhenqing; Traniello, Ian M.; Rana, Seema; Ahmed, Amy Cash; Sankey, Alison; Che, Yang; Robinson, Gene E.

doi:10.1080/01677063.2021.1887173

Cited by 31 publications

(24 citation statements)

References 61 publications

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“…Mutations in Orco do not affect the development of glomeruli in Drosophila [7,23], while Orco mutant ants lack most of the glomeruli found in WT and present a two-third reduced volume of the whole AL [20,21]. In bees, Orco mutants have significantly fewer glomeruli, a smaller volume of the AL, but larger mean individual glomerulus volumes compared to WT [22]. In the flour beetle Tribolium castaneum, Orco knock-down via RNAi at the late larval stage resulted in normal AL glomerulization at adult emergence, but in a heavily reduced glomerulization seven days later, whereas the OSNs still locate normally in the antennae at both ages [40].…”

Section: Discussionmentioning

confidence: 89%

“…For instance, CRISPR-Cas9 has been largely applied to knock-out Orco in different insect species, revealing its primordial function in olfactory transduction, including sex pheromone sensing in moths [17][18][19], but also in the development and maintenance of AL structure, depending on the species. In ants and bees, Orco appears to play a role in OSN development and glomerulus formation [20][21][22], whereas in Drosophila, loss of function of Orco does not affect the glomeruli formation in the AL [7,23].…”

Section: Introductionmentioning

confidence: 99%

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Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth

et al. 2022

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Sex pheromone receptors are crucial in insects for mate finding and contribute to species premating isolation. Many pheromone receptors have been functionally characterized, especially in moths, but loss of function studies are rare. Notably, the potential role of pheromone receptors in the development of the macroglomeruli in the antennal lobe (the brain structures processing pheromone signals) is not known. Here, we used CRISPR-Cas9 to knock-out the receptor for the major component of the sex pheromone of the noctuid moth Spodoptera littoralis, and investigated the resulting effects on electrophysiological responses of peripheral pheromone-sensitive neurons and on the structure of the macroglomeruli. We show that the inactivation of the receptor specifically affected the responses of the corresponding antennal neurons did not impact the number of macroglomeruli in the antennal lobe but reduced the size of the macroglomerulus processing input from neurons tuned to the main pheromone component. We suggest that this mutant neuroanatomical phenotype results from a lack of neuronal activity due to the absence of the pheromone receptor and potentially reduced neural connectivity between peripheral and antennal lobe neurons. This is the first evidence of the role of a moth pheromone receptor in macroglomerulus development and extends our knowledge of the different functions odorant receptors can have in insect neurodevelopment.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth

et al. 2022

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“…Oliver Kobler, Bertram Gerber, and colleagues report a new protocol for the 3 D visualization of transgene expression across the whole body of Drosophila larva (Kobler et al, 2021). Gene Robinson and colleagues examine neurodevelopmental and transcriptomic effects of CRISPR/Cas9induced somatic orco mutation in honey bees (Chen et al, 2021). Charalambos Kyriacou and Rosamund Stanley examine latitudinal cline in a Drosophila courtship song, finding that song interpulse interval is strongly associated with latitude (Stanley & Kyriacou, 2021).…”

Section: Editorialmentioning

confidence: 99%

Transcending boundaries: from quantitative genetics to single genes

Dason

Anreiter

2021

Journal of Neurogenetics

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“…In addition, expression of Orco is required to avoid degeneration of OSNs in the maxillary palps in the vinegar fly ( Drosophila melanogaster ) [ 23 ]. Orco is also essential for maintaining social behavior and the development of antennal lobes in the clonal raider ant ( Ooceraea biroi ) [ 24 ], the Jerdon’s jumping ant ( Harpegnathos saltator ) [ 25 ], and the honey bee ( Apis mellifera ) [ 26 ]. In Blattodea, Orco has been functionally characterized in the German cockroach ( Blatella germanica ) [ 27 ], and in the worker caste of two termite species, a fungus-growing termite ( Odontotermes formosanus ) [ 28 ] and a subterranean termite ( Reticulitermes chinensis ) [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression variation of the odorant receptor co-receptor in the Formosan subterranean termite

2022

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Subterranean termites live in underground colonies with a division of labor among castes (i.e., queens and kings, workers, and soldiers). The function of social colonies relies on sophisticated chemical communication. Olfaction, the sense of smell from food, pathogens, and colony members, plays an important role in their social life. Olfactory plasticity in insects can be induced by long- and short-term environmental perturbations, allowing adaptive responses to the chemical environment according to their physiological and behavioral state. However, there is a paucity of information on the molecular basis of olfaction in termites. In this study, we identified an ortholog encoding the odorant receptor co-receptor (Orco) in the Formosan subterranean termite, Coptotermes formosanus, and examined its expression variation across developmental stages and in response to social conditions. We found that C. formosanus Orco showed conserved sequence and structure compared with other insects. Spatial and temporal analyses showed that the Orco gene was primarily expressed in the antennae, and it was expressed in eggs and all postembryonic developmental stages. The antennal expression of Orco was upregulated in alates (winged reproductives) compared with workers and soldiers. Further, the expression of Orco decreased in workers after starvation for seven days, but it was not affected by the absence of soldiers or different group sizes. Our study reveals the molecular characteristics of Orco in a termite, and the results suggest a link between olfactory sensitivity and nutritional status. Further studies are warranted to better understand the role of Orco in olfactory plasticity and behavioral response.

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Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees

Cited by 31 publications

References 61 publications

Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth

Pheromone Receptor Knock-Out Affects Pheromone Detection and Brain Structure in a Moth

Transcending boundaries: from quantitative genetics to single genes

Molecular characterization and expression variation of the odorant receptor co-receptor in the Formosan subterranean termite

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