NEUROD1 predicts better prognosis in pancreatic cancer revealed by a TILs-based prognostic signature

Abstract

It has been well-defined that tumor-infiltrating lymphocytes (TILs) play critical roles in pancreatic cancer (PaCa) progression. This research aimed to comprehensively explore the composition of TILs in PaCa and their potential clinical significance. A total of 178 samples from the TCGA and 63 samples from the GSE57495 dataset were enrolled in our study. ImmuCellAI was applied to calculate the infiltrating abundance of 24 immune cell types in PaCa and further survival analysis revealed the prognostic values of TILs in PaCa. Moreover, the Hallmark enticement analysis of differentially expressed genes (DEGs) between low- and high-risk groups was performed as well. Immunohistochemistry staining was used to evaluate NEUROD1 expression. As result, different kinds of TILs had distinct infiltrating features. In addition, Specific TILs subsets had notable prognostic values in PaCa. We further established a 6-TILs signature to assess the prognosis of PaCa patients. Kaplan-Meier and Cox regression analyses both suggested the significant prognostic value of the signature in PaCa. Based on the prognostic signature, we screened a great deal of potential prognostic biomarkers and successfully validated NEUROD1 as a novel prognostic biomarker in PaCa. Overall, the current study illuminated the immune cells infiltrating the landscape in PaCa and identified a TILs-dependent signature and NEUROD1 for prognostic prediction in PaCa patients.

NeuroD1-GPX4 signaling leads to ferroptosis resistance in hepatocellular carcinoma

“…One-way ANOVA and Tukey multiple comparisons analyses were performed when more than two groups were compared. shND1: shRNA expression vector targeting NeuroD1; pcCon: pcEF9-Puro; **P < 0.01. https://doi.org/10.1371/journal.pgen.1011098.g005 Previous studies have shown that NeuroD1, originally discovered as a factor regulating neuronal development, is upregulated in several types of cancer, including neuroblastoma, small cell lung cancer, colon cancer, breast cancer, and pancreatic cancer [41,42,[77][78][79][80]. Furthermore, recent studies have shown that it promotes the migration and invasion of neuroendocrine carcinoma and pancreatic cancer cells [39,81].…”