Neurochemical fingerprinting of amygdalostriatal and intra-amygdaloid projections: a tracing–immunofluorescence study in the rat

Wouterlood, Floris G.; Oort, Sanne van; Bloemhard, Lucian; Flierman, Nico A.; Spijkerman, Jorik; Wright, Christopher I.; Beliën, Jeroen A.M.; Groenewegen, H.J.

doi:10.1016/j.jchemneu.2018.11.001

Cited by 3 publications

(4 citation statements)

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“…Glutamatergic cortical afferents target dendritic spines of medium spiny neurons (MSNs) in the striatum ( Bolam et al, 2000 ; Surmeier et al, 2007 ; Kreitzer and Malenka, 2008 ; Silberberg and Bolam, 2015 ). In addition, the striatum receives glutamatergic afferent inputs from the thalamus, subthalamic nucleus, pedunculopontine nucleus, hippocampus, and amygdala ( Smith and Parent, 1986 ; Doig et al, 2010 ; Lodge and Grace, 2011 ; Mathai and Smith, 2011 ; Koshimizu et al, 2013 ; Haber, 2016 ; Wouterlood et al, 2018 ; Assous et al, 2019 ). The main glutamatergic inputs to the MSNs are derived from the brain cortex and thalamus ( Haber, 2016 ).…”

Section: Organization Of the Corticostriatal Pathwaymentioning

confidence: 99%

Role of the Dopaminergic System in the Striatum and Its Association With Functional Recovery or Rehabilitation After Brain Injury

et al. 2021

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Disabilities are estimated to occur in approximately 2% of survivors of traumatic brain injury (TBI) worldwide, and disability may persist even decades after brain injury. Facilitation or modulation of functional recovery is an important goal of rehabilitation in all patients who survive severe TBI. However, this recovery tends to vary among patients because it is affected by the biological and physical characteristics of the patients; the types, doses, and application regimens of the drugs used; and clinical indications. In clinical practice, diverse dopaminergic drugs with various dosing and application procedures are used for TBI. Previous studies have shown that dopamine (DA) neurotransmission is disrupted following moderate to severe TBI and have reported beneficial effects of drugs that affect the dopaminergic system. However, the mechanisms of action of dopaminergic drugs have not been completely clarified, partly because dopaminergic receptor activation can lead to restoration of the pathway of the corticobasal ganglia after injury in brain structures with high densities of these receptors. This review aims to provide an overview of the functionality of the dopaminergic system in the striatum and its roles in functional recovery or rehabilitation after TBI.

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Section: Organization Of the Corticostriatal Pathwaymentioning

confidence: 99%

Role of the Dopaminergic System in the Striatum and Its Association With Functional Recovery or Rehabilitation After Brain Injury

et al. 2021

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“…In cerebral cortex for example, deposition loci of PHA-L or BDA are usually full of labeled neurons of all sorts of pyramidal cells (that are known to be excitatory) and stellate-type neurons (most of which belong to some class of inhibitory interneuron). A consequence of this apparent lack of specificity is that one can expect in 'chemical fingerprinting' studies rather disappointingly low numbers of tracerlabeled axon terminals that express the second, sought-after marker (as we experienced; Wouterlood et al 2018a). In this respect, a 'chemical fingerprinting' study resembles interrogating the entire population of a town to find one lone burglar.…”

Section: Flipping Light Switches: Aav and Neurotransmitter-specific Tracingmentioning

confidence: 89%

“…Sections can be stored long-term at − 20 °C or colder in a cryoprotectant consisting of 20% glycerin and 2% dimethylsulfoxide in 100-mM phosphate buffer, pH 7.4 (Rosene et al 1986). We have successfully stained sections of rat brain stored for more than 20 years under these conditions (Wouterlood et al 2018a).…”

Section: Pha-l Tracing Protocolmentioning

confidence: 99%

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Neuroanatomical tract-tracing techniques that did go viral

Lanciego

Wouterlood

2020

Brain Struct Funct

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Neuroanatomical tracing methods remain fundamental for elucidating the complexity of brain circuits. During the past decades, the technical arsenal at our disposal has been greatly enriched, with a steady supply of fresh arrivals. This paper provides a landscape view of classical and modern tools for tract-tracing purposes. Focus is placed on methods that have gone viral, i.e., became most widespread used and fully reliable. To keep an historical perspective, we start by reviewing one-dimensional, standalone transport-tracing tools; these including today’s two most favorite anterograde neuroanatomical tracers such as Phaseolus vulgaris-leucoagglutinin and biotinylated dextran amine. Next, emphasis is placed on several classical tools widely used for retrograde neuroanatomical tracing purposes, where Fluoro-Gold in our opinion represents the best example. Furthermore, it is worth noting that multi-dimensional paradigms can be designed by combining different tracers or by applying a given tracer together with detecting one or more neurochemical substances, as illustrated here with several examples. Finally, it is without any doubt that we are currently witnessing the unstoppable and spectacular rise of modern molecular-genetic techniques based on the use of modified viruses as delivery vehicles for genetic material, therefore, pushing the tract-tracing field forward into a new era. In summary, here, we aim to provide neuroscientists with the advice and background required when facing a choice on which neuroanatomical tracer—or combination thereof—might be best suited for addressing a given experimental design.

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Examining ventral subiculum and basolateral amygdala projections to the nucleus accumbens shell: Differential expression of VGLuT1, VGLuT2 and VGaT in the rat

Jin

Maddern

Campbell

et al. 2022

Neuroscience Letters

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Neurochemical fingerprinting of amygdalostriatal and intra-amygdaloid projections: a tracing–immunofluorescence study in the rat

Cited by 3 publications

References 67 publications

Role of the Dopaminergic System in the Striatum and Its Association With Functional Recovery or Rehabilitation After Brain Injury

Role of the Dopaminergic System in the Striatum and Its Association With Functional Recovery or Rehabilitation After Brain Injury

Neuroanatomical tract-tracing techniques that did go viral

Examining ventral subiculum and basolateral amygdala projections to the nucleus accumbens shell: Differential expression of VGLuT1, VGLuT2 and VGaT in the rat

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