Neurochemical coding in the small intestine of patients with Crohn's disease.

Belai, Abebech; Boulos, PB; Robson, Tim; Burnstock, Geoffrey

doi:10.1136/gut.40.6.767

Cited by 173 publications

(149 citation statements)

References 31 publications

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“…Percentage of VIP, PACAP and NPY-ergic neurons found in the myenteric plexus in control and pathological sections. there is an increase in the density of nerve elements containing VIP in the wall of the small and the large intestine [11,13]. On the other hand, observations related to the same disease suggest that there are no similar changes in the density of nerve elements containing VIP in the layers of the intestinal wall [29].…”

Section: Discussionmentioning

confidence: 93%

“…Investigations of the enteric nervous system in inflammatory diseases of the large intestine have shown morphological changes in this system. These changes are mostly related with increase, or decrease the number of neurons and density of nerve fibres containing neuropeptides [11][12][13][14][15][16]. As yet there is no data in the literature dealing with the problem of changes in the innervation of human organs affected by cancer.…”

Section: Introductionmentioning

confidence: 99%

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Changes in vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y-ergic structures of the enteric nervous system in the carcinoma of the human large intestine.

Godlewski

Łakomy²

2010

Folia Histochem Cytobiol.

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This investigation was aimed at immunohistochemical analysis of potential changes in the enteric nervous system caused by cancer of the large intestine. In this purpose, neurons and nerve fibers of intestinal plexuses containing neuropeptides: vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating polypeptide (PACAP) and neuropeptide Y (NPY), in pathologically changed part of the large intestine were microscpically observed and compared. Samples were taken from patients operated due to cancer of the sigmoid colon and rectum. The number of neurons and density of nerve fibres containing neuropeptides found in sections with cancer tissues were compared to those observed in sections from the uninvolved intestinal wall. Changes relating to reductions in the number of NPY-ergic neurons and density of nerve fibres in submucous and myenteric plexuses in the sections with cancer tissues (pathological sections) were statistically significant. A statistically similar presence of VIP-ergic and PACAP-ergic neurons in the submucosal and myenteric plexuses was observed in both the pathological and control sections. On the other hand, in the pathological sections, VIP-ergic nerve fibres in the myenteric plexuses and PACAP-ergic nerve fibres in the submucosal and myenteric plexuses were found to be less dense. Analysis revealed changes in pathologically affected part of the large intestine may caused disruption of proper intestinal function. Observed changes in the neural elements which are responsible for relaxation of the intestine may suggest dysfunction in the innervation of this part of the colon.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Changes in vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y-ergic structures of the enteric nervous system in the carcinoma of the human large intestine.

Godlewski

Łakomy²

2010

Folia Histochem Cytobiol.

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“…It should be mentioned that all these observations are at variance with other functional and immunohistochemical studies. Indeed Tomita et al (1998) reported that the colon from patients with UC was more strongly innervated by NANC inhibitory nerves, with NO as major neurotransmitter, than was the normal colon and Belai et al (1997) observed increased VIP, NOS and PACAP immunoreactivity in the myenteric plexus and nerve ®bres of the circular muscle layer of the a icted segment of the ileum of patients with Crohn's disease. The reason for this discrepancy is unclear but it would be of interest to study the role of VIP-ergic neurotransmission in our model.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemical studies have added important information by showing that the content of noradrenaline and neuropeptides in nerve ®bres and neurons can also be altered in patients with in¯ammatory bowel disease (IBD) (Bjorck et al, 1989;Belai et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Generalized loss of inhibitory innervation reverses serotonergic inhibition into excitation in a rabbit model of TNBS‐colitis

Depoortere¹,

Thijs²,

Peeters³

2002

British J Pharmacology

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1 In¯ammation may a ect subpopulations of neurons of the myenteric plexus. 2 In the present study the e ect of trinitrobenzene sulphonic acid (TNBS) induced colitis on nitrergic, purinergic and adrenergic inhibitory neurotransmission was studied as well as the consequences of the related changes on the response of 5-HT agonists using these neurotransmitters to mediate their e ect. 3 Strips from normal and colitis rabbits (135 mg kg 71 TNBS) were subjected to electrical ®eld stimulation (EFS, 0.3 ms, 6V, 0.5 ± 32 Hz, 10 s train). The response was measured isometrically in the absence or presence of L-NAME, suramin, guanethidine, the 5-HT agonists (5-HT 1/5A/7 : 5-carboxamidotryptamine (5-CT), 5-HT 2 : a-methyl-5-HT, 5-HT 3 : 2-methyl-5-HT, 5-HT 4 : 5-methoxytryptamine (5-MeOT)) or a combination. 4 In normal strips L-NAME (1 ± 32 Hz), suramin (0.5 ± 2, 8 Hz) and guanethidine (4, 16, 32 Hz) increased the response to EFS. This e ect was abolished in in¯amed strips and was accompanied by a decrease in nNOS expression. 5 In normal strips all 5-HT agonists induced pronounced (5-CT, a-methyl-5-HT) or small (2-methyl-5-HT, 5-MeOT) inhibitory neural responses. In in¯amed strips this was reversed to cholinergic excitatory responses.6 The e ect of in¯ammation on the 5-HT 4 response was mimicked by preincubation of normal strips with L-NAME or suramin. Accordingly, in in¯amed strips L-NAME or suramin did not a ect the excitatory e ects of 5-MeOT. 7 TNBS-colitis abolishes nitrergic, purinergic and adrenergic neurotransmission. This reverses serotonergic inhibition into excitation.

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“…Immunoreactivity for VIP decreases and substance P increases in tissues from colon of inflammatory bowel disease (IBD) patients compared to controls (Mazumdar and Das 1992;Neunlist et al 2003;Vento et al 2001). Additionally, immunoreactivity for VIP, NO synthase, and neuropeptide Y (NPY) increases in tissues from the ileum of Crohn's disease patients compared to controls (Belai et al 1997).…”

Section: Effects Of Colitis On the Ensmentioning

confidence: 99%

Effects of Inflammation on the Innervation of the Colon

et al. 2013

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Inflammatory bowel diseases (IBD) such as ulcerative colitis and Crohn's disease lead to altered gastrointestinal (GI) function as a consequence of the effects of inflammation on the tissues that comprise the GI tract. Among these tissues are several types of neurons that detect the state of the GI tract, transmit pain, and regulate functions such as motility, secretion, and blood flow. This review article describes the structure and function of the enteric nervous system, which is embedded within the gut wall, the sympathetic motor innervation of the colon and the extrinsic afferent innervation of the colon, and considers the evidence that colitis alters these important sensory and motor systems. These alterations may contribute to the pain and altered bowel habits that accompany IBD.

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Neurochemical coding in the small intestine of patients with Crohn's disease.

Cited by 173 publications

References 31 publications

Changes in vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y-ergic structures of the enteric nervous system in the carcinoma of the human large intestine.

Changes in vasoactive intestinal peptide, pituitary adenylate cyclase-activating polypeptide and neuropeptide Y-ergic structures of the enteric nervous system in the carcinoma of the human large intestine.

Generalized loss of inhibitory innervation reverses serotonergic inhibition into excitation in a rabbit model of TNBS‐colitis

Effects of Inflammation on the Innervation of the Colon

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