Neuroblastoma × spinal cord (NSC) hybrid cell lines resemble developing motor neurons

Abstract

ABSTRACT

An in vivo reporter system for measuring increased inclusion of exon 7 in SMN2 mRNA: potential therapy of SMA

“…However, low transfection efficiency makes them unfavorable for HTS. As an alternative, stable NSC34 cell lines, a widely used fusion motor neuron cell line developed by Cashman et al, 24 may be useful.…”

Alsin, the Product of ALS2 Gene, Suppresses SOD1 Mutant Neurotoxicity through RhoGEF Domain by Interacting with SOD1 Mutants

“…NSC34 cell is a hybrid cell line of motor neurons, derived from embryonic mouse spinal cord cells and mouse neuroblastoma (18,19). These cells are one of the best models for primary cultured motor neurons exhibiting a number of characteristics for primary cultured motor neurons, including generation of action potential, acetylcholine synthesis, storage, and release.…”

“…To confirm that neurotoxicity induced by overexpression of SOD1 mutants involves the motoneuronal cells, we performed a cell death assay using NSC34 cells as an established model for motor neurons. NSC34 is a hybrid cell line generated by fusing embryonic mouse spinal cord cells, in which motor neurons are highly enriched with mouse neuroblastoma cells (18,19). It is used worldwide as a model for primary cultured motor neurons with a number of motoneuronal characteristics, including occurrence of action potentials as well as synthesis, storage, and release of acetylcholine.…”

“…1). First, we found that alsin LF, but not alsin SF, protected NSC34 cells, the model cell line for motor neurons (18), from SOD1 mutant-induced neurotoxicity. The RhoGEF domain is involved in alsin LF-mediated neuroprotection.…”

The LIM/Homeodomain Protein Islet1 Recruits Janus Tyrosine Kinases and Signal Transducer and Activator of Transcription 3 and Stimulates Their Activities

“…When required, cells were stimulated using 40 ng/ml human IL-6 (PeproTech, Rocky Hill, NJ), 100 ng/ml human epidermal growth factor (EGF) (Upstate Biotechnology, Lake Placid, NY), or 2.7 ϫ 10 6 U/ml human LIF. The NSC-34 hybrid cell line, obtained by a fusion of motor neuron-enriched embryonic mouse spinal chord cells with mouse neuroblastoma N18TG cells, was a gift from N. R. Cashman (Cashman et al, 1992). Cells were routinely maintained in DMEM, supplemented with 5% heat-inactivated FBS, 1 mM l-glutamine, and antibiotics (100 U/ml penicillin and 100 ng/ml streptomycin).…”