Neuroblast migration along cellular substrates in the developing porcine brain

Porter, Demisha D.L.; Henry, Sara N.; Ahmed, S. Ansar; Rizzo, Amy L.; Makhlouf, Rita; Gregg, Collin; Morton, Paul D.

doi:10.1016/j.stemcr.2022.07.015

Cited by 6 publications

(4 citation statements)

References 61 publications

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“…Experiments on the 5HT3a-GFP mouse showed CGEderived migratory neurons target several regions, including the dorsal cortex and striatum through P10 (33,34); DCX+ neurons in the P20 ferret brain, a species that first develops gyri postnatally, express CGE-related markers, such as SP8 and SCGN in small migratory streams to PFC and the occipital lobe (35). Here, we found that over half of DCX+ cells within the piglet and human Arc express SP8 and COUP-TFII, consistent with previous reports in the piglet brain (10). Spatial transcriptomic profiling confirmed that the majority of DCX+ neurons in the cortical streams from the Arc were also CGE-derived.…”

Section: Discussionsupporting

confidence: 91%

“…A small population of neuroblasts was observed in the dorsal parenchyma of these brains (7)(8)(9). The piglet SVZ has more complicated structure features including a vascular substrate, suggesting more similarity to human SVZ (10,11). Understanding the evolution of this structure and how it contributes to migratory populations in the postnatal cortex is fundamental to identifying the development of cortical regions important for higher cognitive functions and their potential vulnerabilities to perinatal changes occurring before and after birth.…”

Section: Main Textmentioning

confidence: 98%

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The Neonatal Gyrencephalic Cortex Maintains Regionally Distinct Streams of Neuroblasts

Kim¹,

Sandoval²,

Poddar³

et al. 2023

Preprint

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Neurodevelopmental mechanisms have evolved to support the formation of diverse brain structures, such as in humans, during the perinatal period. Here, we demonstrate that neonatal gyrencephalic brains harbor an expanded subventricular zone, termed the Arc, defined by tiered arrangement of doublecortin (DCX)-expressing neuroblasts and vascular enrichment at the ventricular wall. The Arc is the origin of dorsal and ventral populations of migratory neuroblasts that target multiple regions involved in higher cognitive functions. Arc-derived migratory streams, primarily from the caudal ganglionic eminence, are composed of diverse neuronal subtypes with distinct spatial and migratory-receptor profiles. Our findings indicate the Arc is a structure present in phylogenetically divergent species that supports the expansion of postnatal neuronal migration, contributing to a protracted formation of cortical circuits in gyrencephalic brains.

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Section: Discussionsupporting

confidence: 91%

Section: Main Textmentioning

confidence: 98%

The Neonatal Gyrencephalic Cortex Maintains Regionally Distinct Streams of Neuroblasts

Kim¹,

Sandoval²,

Poddar³

et al. 2023

Preprint

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“…We investigated if the migrating neuroblasts were associated with radial glia or vasculature during their migration, as described in previous work 7,[18][19][20][21][22][23] . To evaluate association with radial glia, we counted instances of putative migratory neurons being in contact with vimentin positive (Vim+) fibers in histological sections.…”

Section: Migrating Neurons May Flexibly Use Vascular Scaffoldsmentioning

confidence: 99%

“…Furthermore, in vivo time-lapse experiments showed that migratory interneurons with bipolar morphology move in many different directions in the intact developing cortex 40,41 . These tangentially migrating interneurons do not seem to be dependent on radial glial scaffolds but instead may be able to switch their migratory strategy between scaffoldassociated and scaffold-free migration, especially once they arrive at the cortex 18,[42][43][44][45] . Finally, in the adult rostral migratory stream (RMS), a major route for newborn neurons in the adult mammalian brain, timelapse experiments showed that almost a third of migration cells exhibited local, "exploratory" dynamics that changed direction frequently 46,47 .…”

Section: Comparison With Other Forms Of Migrationmentioning

confidence: 99%

In vivo imaging in transgenic songbirds reveals superdiffusive neuron migration in the adult brain

Shvedov,

Analoui,

Dafalias

et al. 2023

Preprint

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Neuron migration is a key phase of neurogenesis, critical for the assembly and function of neuronal circuits. In songbirds, this process continues throughout life, but how these newborn neurons disperse through the adult brain is unclear. We addressed this question using in vivo two-photon imaging in transgenic songbirds that express GFP in young neurons. In juvenile and adult birds, migratory cells were present at a high density, traveled in all directions, and made frequent course changes. Notably, these dynamic migration patterns were well fit by a superdiffusive model. Simulations revealed that these diffusion-like dynamics were sufficient to disperse new neurons throughout the song nucleus HVC. These results suggest that diffusion-like migration may underlie the formation and maintenance of nuclear brain structures in the postnatal brain and indicate that transgenic songbirds are a useful resource for future studies into the mechanisms of adult neurogenesis.

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Doublecortin-Expressing Neurons in Human Cerebral Cortex Layer II and Amygdala from Infancy to 100 Years Old

Cai

et al. 2023

Mol Neurobiol

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A cohort of morphologically heterogenous doublecortin immunoreactive (DCX+) "immature neurons" has been identi ed in the cerebral cortex largely around layer II and the amygdala largely in the paralaminar nucleus (PLN) among various mammals. To gain a wide spatiotemporal view on these neurons in humans, we examined layer II and amygdalar DCX + neurons in the brains of infants to 100 year-old individuals. Layer II DCX + neurons occurred throughout the cerebrum in the infants/toddlers, mainly in the temporal lobe in the adolescents and adults, and only in the temporal cortex surrounding the amygdala in the elderly. Amygdalar DCX + neurons occurred in all age groups, localized primarily to the PLN, and reduced in number with age. The small-sized DCX + neurons were unipolar or bipolar, and formed migratory chains extending tangentially, obliquely and inwardly in layers I-III in the cortex, and from the PLN to other nuclei in the amygdala. Morphologically mature-looking neurons were lightly labeled and co-expressed the neuron-speci c nuclear antigen, glutamic acid decarboxylase and nicotinamide adenine dinucleotide phosphate-diaphorase. Taken together, we consider that the layer II and amygdalar DCX + neurons form a slowly developing interneuron system that supports inhibitory network plasticity in the cerebrum in an age/region-dependent manner.

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Neuroblast migration along cellular substrates in the developing porcine brain

Cited by 6 publications

References 61 publications

The Neonatal Gyrencephalic Cortex Maintains Regionally Distinct Streams of Neuroblasts

The Neonatal Gyrencephalic Cortex Maintains Regionally Distinct Streams of Neuroblasts

In vivo imaging in transgenic songbirds reveals superdiffusive neuron migration in the adult brain

Doublecortin-Expressing Neurons in Human Cerebral Cortex Layer II and Amygdala from Infancy to 100 Years Old

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