Neurobehavioral status and abstinence signs in newborns exposed to high doses of caffeine in the prenatal period

Tanabe, Ithallo Sathio Bessoni; Dias, Renise Bastos Farias; Alcântara, Bárbara Fernanda Santos; Tanabe, Eloiza; Figueiredo, Elaine Virgínia Martins de Souza

doi:10.1016/j.placenta.2019.06.355

Cited by 1 publication

(2 citation statements)

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“…The sequenced reads were demultiplexed and trimmed using Claident (v0.2.2019.05.10) by removing reads that included low-quality regions with default settings [28]. To differentiate amplicon sequence variants (ASVs), the trimmed reads were analyzed using the DADA2 pipeline (v. 1.16) in R version 3.5.1, with the settings of “trimLeft = 17, trimRight = 21” (Table S2) [29, 30].…”

Section: Methodsmentioning

confidence: 99%

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Identification of key virus–prokaryote infection pairs that contribute to viral shunt in a freshwater lake

Shen

Tominaga

Tsuchiya

et al. 2023

Preprint

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Viruses infect and kill productive prokaryotes in a density- or frequency-dependent manner and affect carbon cycling. However, the effects of the stratification transition, including the stratified and de-stratified periods, on the changes in prokaryotic/viral communities and the interactions among them remain unclear. We conducted a monthly survey of the surface and deep layers of a large and deep freshwater lake (Lake Biwa, Japan) for a year and analyzed the prokaryotic production and prokaryotic/viral metagenome. Our analysis (including 1 608 prokaryotes and 13 761 viruses) revealed that 19 prokaryotic species, accounting for ~40% of total abundance, might be suppressed by viruses when prokaryotic production is higher. This suggests that a small proportion of prokaryotes contribute to a large amount of prokaryotic abundance, and these prokaryotes are infected and lysed by viruses, driving the viral shunt in the freshwater lake. Furthermore, we found that annual vertical mixing might yield a similar rate of community change between the surface and deep layers. This finding might be valuable in understanding how the communities change when the stratification of freshwater lakes is affected by global warming in the future.

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Section: Methodsmentioning

confidence: 99%

“…ASVs were first classified using the ARB database [31] [32]. The unclassified ASVs were classified using Claident with default settings against the NCBI nucleotide sequence database [28, 33]. ASVs not assigned as prokaryotes (eukaryotes, mitochondria, and chloroplasts) were removed.…”

Section: Methodsmentioning

confidence: 99%