Neurobehavioral effects of long‐term maternal fructose intake in rat offspring

Erbaş, Oytun; Erdoğan, Mümin Alper; Khalilnezhad, Asghar; Gürkan, Fulya Tuzcu; Yiğittürk, Gürkan; Meral, Ayfer; Taşkıran, Dilek

doi:10.1016/j.ijdevneu.2018.07.001

Cited by 30 publications

(26 citation statements)

References 66 publications

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“…Sociability test was performed as previously described with minor modifications (Ellegood & Crawley, 2015; Erbas et al, 2018). Briefly, a Plexiglas cage (40 cm × 90 cm×40 cm) was divided into three equal regions (40 cm × 30 cm×40 cm).…”

Section: Methodsmentioning

confidence: 99%

“…Learning and memory performance of offspring were evaluated by passive avoidance learning (PAL) test as described previously (Erbas et al, 2018). PAL is comprised of fear‐motivated avoidance tasks in which the rat learns to refrain from stepping through a door that seems apparently safer but actually leads into a dark compartment with an electrified grid system that delivers a shock.…”

Section: Methodsmentioning

confidence: 99%

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Exenatide, a GLP‐1 analog, has healing effects on LPS‐induced autism model: Inflammation, oxidative stress, gliosis, cerebral GABA, and serotonin interactions

Solmaz

Tekatas²,

Erdoğan

et al. 2020

Intl J of Devlp Neuroscience

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Previous studies have established anti‐inflammatory, antioxidant, and neuroprotective effects of Exenatide in the central nervous system. Since these mechanisms are thought to have important roles in the pathophysiology of autism, we hypothesized that Exenatide may have healing effects in autism. We tested this hypothesis by examining the effects of Exenatide in an experimental autism model created by lipopolysaccharide (LPS) exposure in the womb, with behavioral tests, histopathological examinations, and biochemical measurements. The autism model was created by administration of LPS (i.p) to pregnant rats on the 10th day of their pregnancy at a dose of 100 µg/kg. On postnatal 21st day, a total of four groups were formed from offspring with regard to sex distribution and treatment. After a 45‐day treatment, behavioral analysis tests were performed on rats. Subsequently, the rats were sacrificed and biochemical analysis [superoxide dismutase, tumor necrotizing factor alpha, nerve growth factor, 5‐hydroxyindoleacetic acid, and glutamic acid decarboxylase‐67] and histopathological analysis were performed. On the 10th day of the intrauterine period, LPS exposure was found to disrupt behavioral findings, increase inflammation and hippocampal gliosis, and decrease 5‐HIAA, GAD‐67, and NGF, especially in male rats. However, among the rats exposed to LPS in the intrauterine period, recipients of Exenatide demonstrated significant amelioration of findings. Exenatide therapy shows positive effects on behavioral disorders in an LPS‐induced autism model. This agent probably exerts its effects by suppressing inflammation and oxidative stress and reducing hippocampal gliosis. In addition, Exenatide has also been shown to positively affect cerebral serotonergic and GABAergic effects.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Exenatide, a GLP‐1 analog, has healing effects on LPS‐induced autism model: Inflammation, oxidative stress, gliosis, cerebral GABA, and serotonin interactions

Solmaz

Tekatas²,

Erdoğan

et al. 2020

Intl J of Devlp Neuroscience

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“…Additionally, neurotrophic factors such as NGF and BDNF play important roles in neuronal growth, survival, and synapse formation [33]. Nevertheless, conflicting results have been obtained about the levels of neurotrophic factors and NRG-1 in individuals with ASD (e.g., increased or unchanged levels), but these findings were based on detection in circulating peripheral blood samples [31,34,35]. In our study, we used brain tissue samples, so the levels found can be considered more representative of concentrations in a living brain.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Prenatal Administration of Tetanus Toxoid on the Sociability and Explorative Behaviors of Rat Offspring: A Preliminary Study

Sünnetçi

Durankuş²,

Albayrak

et al. 2021

Clin Psychopharmacol Neurosci

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Objective Autism spectrum disorder (ASD) is a severely disabling psychiatric disease characterized by impairments in communication and social skills. Although efforts have been made to explore the etiology of ASD, its pathophysiology remains unclear. This issue is rendered more challenging by confounding data about the effects of vaccination on disease etiology. In this study, therefore, we investigated the neurodevelopmental effects of maternal tetanus toxoid administration on rat offspring. We hypothesized that the vaccine affects the sociability and preference for social novelty of rat offspring as well as the production of immunological and neurotrophic factors, including tumor necrosis factor-alfa (TNF-α), neuregulin-1 (NRG-1), neuron growth factor (NGF), and oxytocin. Methods The study involved 12 female and 4 male adult Sprague−Dawley rats (238 ± 10 g), which were assigned to two groups. Group 1 (control group) was given 0.5 ml of normal saline (0.9% NaCl) on the 10th day of pregnancy, whereas Group 2 (experimental group) was administered 0.5 ml of tetanus vaccine (tetanus toxoid, 40 IU). Results Maternal tetanus toxoid administration exerted beneficial effects on the sociability and explorative behaviors of the rats. The brain tissue levels of TNF-α, NGF, NRG-1, and oxytocin were higher in the experimental group than those among the controls. All these significant differences were found in both the male and female rats. Conclusion This study is the first to demonstrate the advantages of tetanus toxoid administration in relation to the sociability and explorative behaviors of rat offspring. The results showed that the vaccine also influences NRG-1, neuregulin, and oxytocin production.

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“…The sociability test was performed as previously described with minor modi cations (Ellegood and Crawley, 2015;Erbas et al, 2018;Moy et al, 2004). Brie y, a Plexiglas cage (40 cm x 90 cm x 40 cm) was divided into three equal regions (40 cm x 30 cm x 40 cm).…”

Section: Behavioral Tests Three-chamber Sociability and Social Noveltmentioning

confidence: 99%

“…The open-eld test was conducted in an open-aired box with dimensions of 50 cm x 50 cm x 40 cm (Erbas et al, 2018). At the beginning of the test, rats were gently placed in the center of the box and freely explored the arena for 5 minutes.…”

Section: Open Eld Testmentioning

confidence: 99%

Chronic Testosterone Administration During Pregnancy Causes Autistic-like Traits in Rat Offspring: Possible Relationships with Cerebral Oxytocin, Dopamine, Serotonin and IGF-1 Levels.

Şenkal

Eryiğit²,

Bozkurt

et al. 2021

Preprint

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Autism spectrum disorder (ASD) is a neurodevelopmental disorder that affects boys three times more frequently than girls. Previous studies have suggested that higher fetal testosterone exposure can cause autism-like behaviors in males. The mechanism of effect of fetal testosterone on autism development is not well known. In the present study, we investigated the relationship between prenatal testosterone exposure and ASD in an experimental study with the use of behavioral tests, histopathological examinations and biochemical measurements performed to compare offspring with and without exposure to testosterone. Female rats were randomly distributed into Group 1 (control, n = 6) and Group 2 (Testosterone undecanoate, n = 6). Female rats were caged with a fertile male (three female/one male) for 2–3 days during the oestrus period. Group 1 rats were given 1 ml/kg % 0.9 NaCl saline on the 10th day of pregnancy, while Group 2 rats were given 250 mg/kg testosterone undecanoate. The dams were allowed to raise their litters until weaning on postnatal day 21 (P21). On P21 , forty littermates (10 male control, ten female control, ten male Testosterone-exposed, and ten female Testosterone-exposed) were randomly separated and housed. These animals underwent behavioral testing in adulthood on P50. Subsequently, the rats were sacrificed. Biochemical analyses [Brain tissue 5-Hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA), Insulin-like growth factor-1 (IGF-1), and oxytocin] and histopathological analyses were performed. Analysis of the behavioral tests (three-chamber social test, open field test) revealed significant differences among the groups, with the testosterone-exposed groups demonstrating autistic traits at a greater degree. Histologically, hippocampal CA1 and CA3 regions displayed significant alterations such as gliosis and neuronal cell death in the testosterone-exposed groups compared to controls. Brain levels of tissue 5-HIAA, HVA, IGF-1 increased while oxytocin level decreased in the testosterone-exposed groups. These results suggest a possible link between chronic prenatal testosterone exposure and neurodevelopmental disorders such as ASD. Testosterone exposure and autism-like traits can be linked to dopamine, serotonin, IGF-1 increase, and oxytocin decrease.

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Neurobehavioral effects of long‐term maternal fructose intake in rat offspring

Abstract: These results suggest a possible link between the chronic maternal metabolic stress, such as long-term fructose intake, and neurodevelopmental disturbances in the offspring.

Cited by 30 publications

References 66 publications

Exenatide, a GLP‐1 analog, has healing effects on LPS‐induced autism model: Inflammation, oxidative stress, gliosis, cerebral GABA, and serotonin interactions

Exenatide, a GLP‐1 analog, has healing effects on LPS‐induced autism model: Inflammation, oxidative stress, gliosis, cerebral GABA, and serotonin interactions

Effects of the Prenatal Administration of Tetanus Toxoid on the Sociability and Explorative Behaviors of Rat Offspring: A Preliminary Study

Chronic Testosterone Administration During Pregnancy Causes Autistic-like Traits in Rat Offspring: Possible Relationships with Cerebral Oxytocin, Dopamine, Serotonin and IGF-1 Levels.

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