Neuro-optical microfluidic platform to study injury and regeneration of single axons

Kim, Young Tae; Karthikeyan, Kailash; Chirvi, Sajal; Davé, Digant P.

doi:10.1039/b903720a

Cited by 77 publications

(75 citation statements)

References 33 publications

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“…To visualize tumor cell capture via the anti-EGFR aptamer substrates, these were placed on a custom-designed neurooptical microfluidic platform, and the interaction between tumor cells and surface-grafted aptamers was monitored as described previously (35). Briefly, the substrates were placed on the platform, which maintained 5% CO 2 at 37°C and high humidity for live cell imaging.…”

Section: Monitoring Of the Dynamic Interactions Between The Tumor Celmentioning

confidence: 99%

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

et al. 2010

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Exposing rare but highly malignant tumor cells that migrate from the primary tumor mass into adjacent tissue(s) or circulate in the bloodstream is critical for early detection and effective intervention(s). Here, we report on an aptamer-based strategy directed against epidermal growth factor receptor (EGFR), the most common oncogene in glioblastoma (GBM), to detect these deadly tumor cells. GBMs are characterized by diffuse infiltration into normal brain regions, and the inability to detect GBM cells renders the disease surgically incurable with a median survival of just 14.2 months. To test the sensitivity and specificity of our platform, anti-EGFR RNA aptamers were immobilized on chemically modified glass surfaces. Cells tested included primary human GBM cells expressing high levels of the wild-type EGFR, as well as genetically engineered murine glioma cells overexpressing the most common EGFR mutant (EGFRvIII lacking exons 2-7) in Ink4a/Arf-deficient astrocytes. We found that surfaces functionalized with anti-EGFR aptamers could capture both the human and murine GBM cells with high sensitivity and specificity. Our findings show how novel aptamer substrates could be used to determine whether surgical resection margins are free of tumor cells, or more widely for detecting tumor cells circulating in peripheral blood to improve early detection and/or monitoring residual disease after treatment.

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Section: Monitoring Of the Dynamic Interactions Between The Tumor Celmentioning

confidence: 99%

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

et al. 2010

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“…Nevertheless the presented device is 10-100-fold more economical with cell material than other compartmentalized systems. [10][11][12][13][14][15][16][17][18][19][20]39 This also highlights the advantage of using aspiration for cell arraying, a process which can readily manage minimal cell suspension volumes (e.g. 1 mL).…”

Section: Microfluidic Arraying With Single Neuron Precisionmentioning

confidence: 97%

Microfluidic construction of minimalistic neuronal co-cultures

et al. 2013

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In this paper we present compartmentalized neuron arraying (CNA) microfluidic circuits for the preparation of neuronal networks using minimal cellular inputs (10-100-fold less than existing systems).The approach combines the benefits of microfluidics for precision single cell handling with biomaterial patterning for the long term maintenance of neuronal arrangements. A differential flow principle was used for cell metering and loading along linear arrays. An innovative water masking technique was developed for the inclusion of aligned biomaterial patterns within the microfluidic environment. For patterning primary neurons the technique involved the use of meniscus-pinning micropillars to align a water mask for plasma stencilling a poly-amine coating. The approach was extended for patterning the human SH-SY5Y neuroblastoma cell line using a poly(ethylene glycol) (PEG) back-fill and for dopaminergic LUHMES neuronal precursors by the further addition of a fibronectin coating. The patterning efficiency E patt was .75% during lengthy in chip culture, with y85% of the outgrowth channels occupied by neurites. Neurons were also cultured in next generation circuits which enable neurite guidance into all outgrowth channels for the formation of extensive inter-compartment networks. Fluidic isolation protocols were developed for the rapid and sustained treatment of the different cellular and sub-cellular compartments. In summary, this research demonstrates widely applicable microfluidic methods for the construction of compartmentalized brain models with single cell precision. These minimalistic ex vivo tissue constructs pave the way for high throughput experimentation to gain deeper insights into pathological processes such as Alzheimer and Parkinson Diseases, as well as neuronal development and function in health.

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“…The laser dissector based on a sub-nanosecond pulsed UV-A source, permit the three-dimensionally confined ablation just like systems based on two-photon absorption processes. However, it has the advantage of delivering extremely low average power (few mW) to the sample, thereby minimizing undesirable thermal effects (Colombelli et al 2007;Kim et al 2009). The developed holographic optical tweezers based on a CW infrared laser and a spatial light modulator represent a cheap and easy to implement solution.…”

Section: Discussionmentioning

confidence: 99%

Integration of Optical Manipulation and Electrophysiological Tools to Modulate and Record Activity in Neural Networks

Difato

Schibalsky

Benfenati

et al. 2011

International Journal of Optomechatronics

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We present an optical system that combines IR (1064 nm) holographic optical tweezers with a sub-nanosecond-pulsed UV (355 nm) laser microdissector for the optical manipulation of single neurons and entire networks both on transparent and non-transparent substrates in vitro. The phase-modulated laser beam can illuminate the sample concurrently or independently from above or below assuring compatibility with different types of microelectrode array and patch-clamp electrophysiology. By combining electrophysiological and optical tools, neural activity in response to localized stimuli or injury can be studied and quantified at sub-cellular, cellular, and network level.

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Neuro-optical microfluidic platform to study injury and regeneration of single axons

Cited by 77 publications

References 33 publications

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

Surface-Immobilized Aptamers for Cancer Cell Isolation and Microscopic Cytology

Microfluidic construction of minimalistic neuronal co-cultures

Integration of Optical Manipulation and Electrophysiological Tools to Modulate and Record Activity in Neural Networks

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