Neuro- and Hepatoprotective Effects of Methylene Blue in Rats Treated with Lipopolysaccharide Endotoxin

Abdel-Salam, Omar M.E.; Sleem, Amany A.; Youness, Eman R.; Mohammed, Nadia A.; Shaffie, Nermeen; Yassen, Noha N.

doi:10.20455/ros.2018.849

Cited by 2 publications

(1 citation statement)

References 55 publications

(66 reference statements)

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“…Mayer et al [16] suggested that MethyB acts primarily as a direct inhibitor of nitric oxide synthase, but is only a poor inhibitor of soluble guanylyl cyclase. MethyB has been shown to inhibit the production of nitric oxide via the inhibition of nitric oxide synthases both in vitro and in vivo [16,17] and to decrease brain nitrite/nitrate [39] and serum nitric oxide concentrations [40] .…”

Section: Dmentioning

confidence: 99%

Protection by L-arginine against Epinephrine-induced Arrhythmia and Cardiotoxicity

M.E. Abdel-Salam,

Baset,

Omara

et al. 2024

Innov Discov

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Nitric oxide is important in control of coronary blood flow, cardiomyocyte contractility, cardiac rhythm, and thrombogenicity. We aimed to investigate the effect of the nitric oxide precursor L-arginine on cardiac arrhythmia and myocardial injury induced by epinephrine in male Sprague-Dawley rats. The possible modulation of L-arginine effects by methylene blue (MethyB), a nitric oxide synthase inhibitor was also examined. Cardiac arrhythmia was induced with 10μg/kg epinephrine given intravenously (i.v.). L-arginine (200mg/kg) or L-arginine and MethyB (100mg/kg) were intraperitoneally (i.p.) administered prior to i.v. epinephrine. Other groups received i.p. saline, only L-arginine or only MethyB (100mg/kg). Results showed that compared with the saline control, epinephrine caused a significant bradycardia (188.7±24.4 vs. 405.6±1.19beats/min), increased QRS duration (0.039±0.006 vs. 0.0185±0.0001s), decreased R wave amplitude (0.18±0.01 vs. 0.23±0.011mv), and ventricular extrasystoles. L-arginine alone showed no significant effect on heart rate (380.8±10.0 vs. 405.6±1.19beats/min) but increased QRS duration (0.029±0.0002 vs. 0.0185±0.0001s), and R wave amplitude (0.47±0.01 vs. 0.23±0.011mv). L-arginine given prior to epinephrine prevented bradycardia, shortened the duration of arrhythmia and decreased the number of extrasystoles. The administration of L-arginine and MethyB almost completely suppressed epinephrine-induced ventricular arrhythmias. Epinephrine caused severe degeneration of muscle fibres, widening of intercellular spaces, cellular infiltrations and interstitial haemorrhage. L-arginine markedly attenuated, while L-arginine/MethyB completely prevented these changes. It is concluded that L-arginine protects against cardiac arrhythmias and cardiac tissue damage caused by epinephrine.

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Section: Dmentioning

confidence: 99%

Protection by L-arginine against Epinephrine-induced Arrhythmia and Cardiotoxicity

M.E. Abdel-Salam,

Baset,

Omara

et al. 2024

Innov Discov

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Methylene Blue Protects Against Acute Ethanol-Induced Oxidative Stress and Organ Damage

et al. 2021

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Ethanol (EtOH) intake is an important global health problem which affects many organs such as the brain, liver and stomach. The aim of the study was to examine the effect of the redox dye methylene blue (MethyB) on oxidative stress and histologic damage to the liver, gastric mucosa, and brain induced high dose ethanol (EtOH). Male rats were treated with EtOH (2 ml/rat, 96%) via intragastric route (for two consecutive days). MethyB (20 or 40 mg/kg, intraperitoneally) was given immediately after EtOH administration. The control group received saline. Rats were euthanized three hours after the last treatment. Brain and liver levels of malondialdehyde (MDA), reduced glutathione (GSH), and paraoxonase-1 (PON-1) as well as brain 5-lipoxygenase (5-LOX) and butyrylcholinesterase (BChE) were determined. Histopathological assessment of brain, liver and gastric damage was done. Results indicated that compared to saline treated animals, EtOH caused significant increase in MDA, along with decreased GSH and PON-1 activity in brain and liver. Additionally, it significantly increased 5-LOX and decreased brain BChE activity. The EtOH group showed the presence of dead and red neurons, and damage of glial cells. The liver exhibited vacuolar degeneration, apoptotic hepatocytes and foci of necrosis. The gastric mucosa showed areas of tissue damage, mucosal atrophy, and loss of normal architecture of glandular cells. The EtOH induced biochemical and histopathological alterations were alleviated after treatment with MethyB at a dose-dependent manner. These results demonstrate that MethyB is able to protect against from acute effects of EtOH on brain, liver and gastric tissue via an antioxidant action. MethyB might be of value in reducing tissue injury in acute EtOH intoxication.

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Neuro- and Hepatoprotective Effects of Methylene Blue in Rats Treated with Lipopolysaccharide Endotoxin

Cited by 2 publications

References 55 publications

Protection by L-arginine against Epinephrine-induced Arrhythmia and Cardiotoxicity

Protection by L-arginine against Epinephrine-induced Arrhythmia and Cardiotoxicity

Methylene Blue Protects Against Acute Ethanol-Induced Oxidative Stress and Organ Damage

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