Neurite outgrowth on well-characterized surfaces: preparation and characterization of chemically and spatially controlled fibronectin and RGD substrates with good bioactivity

Peptide-based biopolymers in biomedicine and biotechnology

“…RGD sequences present on peptide amphiphiles [349], hyaluronan hydrogels [350], dextran [351], collagen [352], poly-l-lysinegraft-(polyethylene glycol) copolymers [353], poly(lactic acid-co-lysine) [354], poly(ethylene glycol)-poly(lactic acid) diblock copolymers [355], acrylic terpolymers [356], and polyurethanes [357] have been reported to promote cell adhesion to a variety of cell types, including neurons [353,358], osteoblasts [359,360], endothelial cells [361], and fibroblasts [350]. In some cases, the RGD sequences can also promote other cellular functions such as matrix mineralization of osteogenic cells [359,360] and neurite outgrowth [358]. Other peptide sequences representing important domains of laminin and fibronectin such as IKVAV, YIGSR, and KNEED also promote the attachment of neuronal and endothelial cells [8][9][10][11].…”

A Simplified Micropatterning Method for Straight-line Neurite Extension of Cultured Hippocampal Neurons

“…Researchers in neuroscience and engineering fields have developed two general methods for assembling neuronal networks along defined geometries: chemical patterning with cell adhesive molecules on a substrate, such as with microcontact printing, [6][7][8][9] and physical patterning using three-dimensional structures, such as with microfluidic devices. [10][11][12][13][14] Microcontact printing on surface-modified substrates has been used to immobilize cell adhesive molecules in a micron-scale pattern for the study of neurite growth.…”

“…[10][11][12][13][14] Microcontact printing on surface-modified substrates has been used to immobilize cell adhesive molecules in a micron-scale pattern for the study of neurite growth. 9 The method requires the complicated process of chemical modification. The use of microfluidic devices for detailed observations of neurites and growth cones using non-fluorescent imaging, such as phase-contrast or differential interference contrast (DIC) microscopy, 11,14 has been impeded by the effects of light refraction from microfluidic structures.…”

Optimization of protein patterns for neuronal cell culture applications

“…Surface patterning offers the opportunity to control the spatial distribution of proteins and tune cellular events including cell attachment, [9][10][11][12] process outgrowth, [13][14][15] and other important morphological changes to specific substrate regions. At present, there are a number of soft-lithographic techniques 16 that can be utilized to pattern surfaces on the micrometer scale with polydimethoxysilane (PDMS) devices including microfluidic networks (lFN), 17 plasma-initiated patterning (lPIP), 18,19 replica molding (REM), 20,21 micro-molding in capillaries (MIMIC), 21 and micro-contact printing (lCP).…”

“…In previous studies, we have shown that lCP can create micrometer to sub-micrometer scale surface patterns from a wide variety of biomolecular inks, including extracellular matrix (ECM) proteins, biotin, strepavidin, and immunoglobulins (IgG) on polymeric, metallic, and inorganic surfaces with high spatial resolution. 14,[24][25][26] It is important during the substrate patterning process that the biological activity and spatial contrast of the immobilized biomolecules are properly preserved and not compromised. It has been shown that the properties of the substrate material, including surface roughness, chemical functionality, wettability, and rigidity, as well as the properties of the biomolecules themselves, including size, charge, and structure, play a major role in the activity of the immobilized biomolecules.…”