Neurite outgrowth of PC12 cells on diX (parylene) family materials

Abstract

We investigated neuronal cell differentiation, particularly neurite outgrowth, on the surface of diX H and diX AM using an in vitro examination of a neuron-like rat pheochromocytoma cell line, PC12. diX H and diX AM are in the parylene family of diX C (or Parylene-C), which is widely used as a novel coating material to insulate neural electrodes, and they have been recently commercialized; diX H and diX AM offer different features of biocompatibility. Previously, we found that these new parylene materials have high cell adhesiveness to neuronal cells whereas the adhesiveness of diX C is extremely low. However, their cell differentiation remains unknown although neuronal cell differentiation plays a crucial role in their development and regeneration. This study showed that almost all PC12 cells adhering to the surface of diX AM and diX H were differentiated, but the neurite outgrowth was significantly larger on diX H than that on diX AM and a conventional polystyrene culture dish. The result suggests that diX H may be advantageous as a biocompatible coating material for a scaffold, which can be used on virtually any substrate to get various configurations in neural devices.

Application of aurintricarboxylic acid for the adherence of mouse P19 neurons and primary hippocampal neurons to noncoated surface in serum‐free culture

“…Recently, some new parylene polymers have been used on tissue engineering and biosensor for neuron adhesive growth in culture. These polymers [poly‐chloro‐para‐xylylene (diX C), poly‐monoaminomethyl‐para‐xylylene (diX AM), and poly‐monoaldehyde‐para‐xylylene (diX H)] can be coated on SiO 2 surface in combination with proteins and UV irradiation and have been shown to markedly improve the neuronal cell attachment on micropatterned surfaces 10–13…”

Evaluation of parylene derivatives for use as biomaterials for human astrocyte cell patterning

“…Kato et al observed no cell attachment to parylene-C or parylene-A, however, cell attachment was observed to parylene-AM and parylene-H. This work was expanded on by Hoshino et al who examined the outgrowth of neurites from PC12 cells on parylene-AM and parylene-H [50]. Hoshino et al found that neurite outgrowth was greatest on the parylene-H.…”