Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12

Wang, Gang; Yang, Kai

doi:10.3892/ol.2020.12252

Cited by 3 publications

(1 citation statement)

References 29 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 26 The research of Wang G et al indicated that NRSN2 enhances the proliferation, invasion, and migration of colorectal cancer cells. 27 Besides, a research has revealed that inhibition of NRSN2 restrains the proliferation, migration, and invasion of esophageal squamous cell carcinoma. 24 Congruously, our research displayed that NRSN2 knockdown restrained the proliferation, migration, and invasion of HPV-transfected LC cells.…”

Section: Discussionmentioning

confidence: 99%

NRSN2 promotes the malignant behavior of HPV-transfected laryngeal carcinoma cells through AMPK/ULK1 pathway mediated autophagy activation

Guo,

Wen,

et al. 2024

Cancer Biology & Therapy

View full text Add to dashboard Cite

Neurensin-2 (NRSN2) performs a pro-carcinogenic function in multiple cancers. However, the function of NRSN2 in HPV-infected laryngeal carcinoma (LC) remains unclear. HPV transfection was performed in LC cells. The mRNA and protein levels were monitored using RT-qPCR, immunoblotting, and IF. Cell viability and proliferation were found using the CCK-8 assay and Edu staining. Cell invasion, migration, and apoptosis were probed using the Transwell, wound healing, and flow cytometry, respectively. The autophagosome was observed using TEM. NRSN2 was overexpressed in HPV-transfected LC cells. Inhibition of NRSN2 restrained the autophagy and malignant behavior of HPV-transfected LC cells. Meanwhile, the inhibition of AMPK/ULK1 pathway limited the increased autophagy of HPV-transfected LC cells caused by NRSN2 overexpression. Furthermore, NRSN2 knockdown inhibits autophagy by suppressing AMPK/ULK1 pathway, thereby restraining the malignant behavior of HPV-transfected LC cells. Our research confirmed that HPV transfection increased the autophagy and malignant behavior of LC cells by regulating the NRSN2-mediated activation of the AMPK/ULK1 pathway, offering a new target for cure of LC.

show abstract

Section: Discussionmentioning

confidence: 99%

NRSN2 promotes the malignant behavior of HPV-transfected laryngeal carcinoma cells through AMPK/ULK1 pathway mediated autophagy activation

Guo,

Wen,

et al. 2024

Cancer Biology & Therapy

View full text Add to dashboard Cite

show abstract

E2F1-activated NRSN2 promotes esophageal squamous cell carcinoma progression through AKT/mTOR pathway

Yan

et al. 2022

Pathology - Research and Practice

View full text Add to dashboard Cite

NRSN2 is a Prognostic Biomarker in Gastric Cancer and Facilitates the Growth and Migration of Gastric Cancer Cells

Ren

Chen

et al. 2023

PPL

View full text Add to dashboard Cite

Background: Neurensin-2 (NRSN2) is reported to be associated with the progression of many tumors. This work aimed at investigating the biological function and prognostic significance of NRSN2 in gastric cancer (GC). Methods: NRSN2 expression in various cancer tissue was analyzed by the TIMER database. NRSN2 expression in GC tissue samples of different groups was analyzed by the UALCAN database. The survival analysis was performed with the Kaplan-Meier database. NRSN2 expression in GC tissues and cell lines was measured by qRT-PCR and Western blot. CCK-8, Transwell and scratch healing assays were conducted to detect the proliferative, migrative and invasive capabilities of GC cells, respectively. The LinkedOmics database and StarBase database were utilized to analyze the related genes with NRSN2 in GC. The association of NRSN2 expression with tumor immune infiltrating cells and molecular markers of immune cells was investigated with the TIMER database. Results: NRSN2 expression was up-regulated in GC tissues, which was correlated with GC tumor grade, lymph node metastasis, and TP53 mutation. The prognosis of GC patients with high NRSN2 expression was worse than those of the patients with low NRSN2 expression. NRSN2 expression was also associated with the TNM stage, and Lauren subtype of GC patients. NRSN2 overexpression promoted the growth, migration and invasion of GC cells lines; knocking down NRSN2 worked oppositely. NRSN2 expression in GC was associated with Wnt, p53, and NOD-like receptor signaling pathways. NRSN2 expression was also significantly associated with the infiltration of CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells in the GC microenvironment. Conclusion: NRSN2 expression in GC tissues is up-regulated, which correlates with a poor prognosis and immune cell infiltration of GC patients. NRSN2 facilitates the growth and aggressiveness of GC cells, implying that it may be a diagnostic biomarker and therapy target for GC. conclusion: NRSN2 expressions in GC tissues are up-regulated, which correlates with a poor prognosis and tumor immune infiltration in GC patients. NRSN2 can expedite the growth and metastasis of GC cells, implying that it may be a diagnostic and prognostic biomarker of GC.

show abstract

Neurensin‑2 promotes proliferation, invasion and migration of colorectal cancer cells via interaction with SOX12

Cited by 3 publications

References 29 publications

NRSN2 promotes the malignant behavior of HPV-transfected laryngeal carcinoma cells through AMPK/ULK1 pathway mediated autophagy activation

NRSN2 promotes the malignant behavior of HPV-transfected laryngeal carcinoma cells through AMPK/ULK1 pathway mediated autophagy activation

E2F1-activated NRSN2 promotes esophageal squamous cell carcinoma progression through AKT/mTOR pathway

NRSN2 is a Prognostic Biomarker in Gastric Cancer and Facilitates the Growth and Migration of Gastric Cancer Cells

Contact Info

Product

Resources

About