Neuregulins stimulate the functional expression of Ca
            <sup>2+</sup>
            -activated K
            <sup>+</sup>
            channels in developing chicken parasympathetic neurons

Subramony, Priya; Dryer, Stuart E.

doi:10.1073/pnas.94.11.5934

Cited by 34 publications

(36 citation statements)

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“…Chick CG neurons were dissociated at E9 or E11 as described previously (16,21,(23)(24)(25). For coculture experiments, iris tissue was dissociated at E13 and cultured for 2 days resulting in formation of a confluent layer of myotubes.…”

Section: Methodsmentioning

confidence: 99%

“…Recombinant ␤-NEU1 and ␣-neuregulin peptides were obtained from R & D Systems and added to culture media as indicated. Note that in our previous studies (21,24), recombinant ␤-NEU1 peptide was provided by Gerald This paper was submitted directly (Track II) to the PNAS office.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously shown that ␤-NEU1 can stimulate the development of functional K Ca in CG neurons developing in vitro (21,25). However, experiments carried out in dissociated cell culture must be interpreted with caution.…”

Section: ␤-Neu1mentioning

confidence: 99%

“…Finally, application of recombinant ␤-NEU1 can increase the density of functional K Ca in cultured CG neurons, at least at high concentrations (21). However, this effect also can be evoked by high concentrations of other erbB ligands such as EGF and transforming growth factor ␣ (TGF␣), but not by ␣-neuregulin (21). In addition, it is not known whether ␤-Neu1 transcripts are expressed in the Edinger-Westphal nucleus at developmental stages that coincide with the normal development of K Ca .…”

mentioning

confidence: 98%

“…Second, functional erbB receptors are expressed in chick CG neurons (20). Finally, application of recombinant ␤-NEU1 can increase the density of functional K Ca in cultured CG neurons, at least at high concentrations (21). However, this effect also can be evoked by high concentrations of other erbB ligands such as EGF and transforming growth factor ␣ (TGF␣), but not by ␣-neuregulin (21).…”

mentioning

confidence: 99%

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β-Neuregulin-1 is required for the in vivo development of functional Ca ²⁺ -activated K ⁺ channels in parasympathetic neurons

Cameron

Dryer

2001

Proc. Natl. Acad. Sci. U.S.A.

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The development of functional Ca 2؉ -activated K ؉ channels (KCa) in chick ciliary ganglion (CG) neurons requires interactions with afferent preganglionic nerve terminals. Here we show that the essential preganglionic differentiation factor is an isoform of ␤-neuregulin-1. ␤-Neuregulin-1 transcripts are expressed in the midbrain preganglionic Edinger-Westphal nucleus at developmental stages that coincide with or precede the normal onset of macroscopic K Ca in CG neurons. Injection of ␤-neuregulin-1 peptide into the brains of developing embryos evoked a robust stimulation of functional KCa channels at stages before the normal appearance of these channels in CG neurons developing in vivo. Conversely, injection of a neutralizing antiserum specific for ␤-neuregulin-1 inhibited the development of KCa channels in CG neurons. Low concentrations of ␤-neuregulin-1 evoked a robust increase in whole-cell KCa in CG neurons cocultured with iris target tissues. By contrast, culturing CG neurons with iris cells or low concentrations of ␤-neuregulin-1 by themselves was insufficient to stimulate KCa. These data suggest that the preganglionic factor required for the development of K Ca in ciliary ganglion neurons is an isoform of ␤-neuregulin-1, and that this factor acts in concert with targetderived trophic molecules to regulate the differentiation of excitability.ciliary ganglion ͉ slowpoke ͉ trophic factor ͉ neuregulins ͉ transforming growth factor ␤

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: ␤-Neu1mentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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β-Neuregulin-1 is required for the in vivo development of functional Ca ²⁺ -activated K ⁺ channels in parasympathetic neurons

Cameron

Dryer

2001

Proc. Natl. Acad. Sci. U.S.A.

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Nitric oxide and target-organ control in the autonomic nervous system: Anatomical distribution, spatiotemporal signaling, and neuroeffector maintenance

Blottner

1999

J. Neurosci. Res.

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Recent neuroanatomical studies, neurochemical coding and physiological findings of multiple cotransmitter actions and/or receptor patterns, and the characterization of synaptic molecules and nitrergic (NOergic) signaling mechanisms may help for a better understanding of target-organ control in the autonomic nervous system. Thus, nitric oxide (NO) synthase, which generates the freely diffusible and short-lived messenger NO and expression of neurotrophic proteins (e.g., neurotrophins, glial cell-line-derived neurotrophic factor, fibroblast growth factors) in autonomic neural pathways or target organs suggest unique actions in autonomic neurotransmission. In central NOergic pathways, NO may serve as spatial (volume) messenger within hierarchically ordered autonomic neuron pools and convergent/divergent pathways for synchronized autonomic outflow. Likewise, NO modulates intraganglionic and interaxonal transmission and postganglionic activity including long-term potentiation. In the visceral targets, NO appears to be a spatial modulator in local intrinsic networks or at varicose terminals. In endocrine glands, NO possibly acts as synaptic coactivator or inhibitor, as a cotransmitter affecting stimulus-coupled exocytosis, or as a local vasoactive signal. The short-term neural messenger NO may also induce diffusible target-derived long-term neurotrophic signals, thereby supporting neuroeffector maintenance and plasticity, if not synaptic efficacy, in autonomic target-organ control.

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Role of cell-cell interactions in the developmental regulation of Ca2+-activated K+ currents in vertebrate neurons

Dryer

1998

J. Neurobiol.

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The functional expression of the Ca2+‐activated K+ current (IK[Ca]) is dependent on cell‐cell interactions in developing chick autonomic neurons. In chick ciliary ganglion (CG) neurons, expression of macroscopic IK[Ca] coincides with the formation of synapses with target tissues. CG neurons that develop in vivo in the absence of normal target tissues fail to express functional IK[Ca], although voltage‐activated Ca2+ currents and most other ionic currents are expressed at normal amplitudes and densities. CG neurons placed in cell culture prior to formation of synapses with target tissues also fail to express macroscopic IK[Ca]. However, CG neurons cultured in the presence of a heat‐ and trypsin‐sensitive extract of target tissues express IK[Ca] at normal levels. Similarly, interactions with target tissue appear to regulate the expression of whole‐cell IK[Ca] in developing chick sympathetic ganglion neurons, although the relevant trophic factors appear to be different from those required by CG neurons. In addition to target tissue interactions, an intact preganglionic innervation is required for the normal in vivo development of IK[Ca] in chick CG neurons. The trophic effects of the afferent innervation do not require synaptic activation of the CG neurons, indicating secretion of a trophic factor, possibly an isoform of β‐neuregulin. The results are consistent with the hypothesis that target‐ and nerve terminal‐derived trophic factors interact at a posttranslational level in the regulation of a functional IK[Ca]. Together, this body of data demonstrates an essential role for cell‐cell interactions in the differentiation of neuronal excitability. © 1998 John Wiley & Sons, Inc. J Neurobiol 37: 23–36, 1998

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Neuregulins stimulate the functional expression of Ca ²⁺ -activated K ⁺ channels in developing chicken parasympathetic neurons

Cited by 34 publications

References 50 publications

β-Neuregulin-1 is required for the in vivo development of functional Ca ²⁺ -activated K ⁺ channels in parasympathetic neurons

β-Neuregulin-1 is required for the in vivo development of functional Ca ²⁺ -activated K ⁺ channels in parasympathetic neurons

Nitric oxide and target-organ control in the autonomic nervous system: Anatomical distribution, spatiotemporal signaling, and neuroeffector maintenance

Role of cell-cell interactions in the developmental regulation of Ca2+-activated K+ currents in vertebrate neurons

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Neuregulins stimulate the functional expression of Ca 2+ -activated K + channels in developing chicken parasympathetic neurons

Cited by 34 publications

References 50 publications

β-Neuregulin-1 is required for the in vivo development of functional Ca 2+ -activated K + channels in parasympathetic neurons

β-Neuregulin-1 is required for the in vivo development of functional Ca 2+ -activated K + channels in parasympathetic neurons

Nitric oxide and target-organ control in the autonomic nervous system: Anatomical distribution, spatiotemporal signaling, and neuroeffector maintenance

Role of cell-cell interactions in the developmental regulation of Ca2+-activated K+ currents in vertebrate neurons

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Neuregulins stimulate the functional expression of Ca ²⁺ -activated K ⁺ channels in developing chicken parasympathetic neurons

β-Neuregulin-1 is required for the in vivo development of functional Ca ²⁺ -activated K ⁺ channels in parasympathetic neurons

β-Neuregulin-1 is required for the in vivo development of functional Ca ²⁺ -activated K ⁺ channels in parasympathetic neurons