Neuralized Promotes Basal to Apical Transcytosis of Delta in Epithelial Cells

Benhra, Najate; Vignaux, Françoise; Dussert, Aurore; Schweisguth, François; Borgne, Roland Le

doi:10.1091/mbc.e09-11-0926

Cited by 57 publications

(73 citation statements)

References 40 publications

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“…5A-CЈ). Although the Rab11 function during pI cell division has not been directly tested owing to its role in cytokinesis, several findings indicate that the Delta ligand transits the Rab11 endosome to reach the pI daughter cell interface to promote Notch signalling and the specification of the pIIa cell fate (Benhra et al, 2011;Benhra et al, 2010;Emery et al, 2005;Jafar-Nejad et al, 2005). Accordingly, we observed that fluorescently labelled internalized 2663 RESEARCH ARTICLE Centrosome movement regulation Delta antibodies can be found localized around the anterior pI centrosome during cytokinesis (Fig.…”

Section: Crmp Controls the Duration Of Rab11 Accumulation Around The mentioning

confidence: 68%

“…In parallel, Delta is activated in the anterior pI daughter by a Neur-dependent mechanism. Neur promotes Delta endocytosis in the anterior pI daughter cell (Benhra et al, 2010;Le Borgne and Schweisguth, 2003b). The Delta recycling to the apical pIIa/pIIb interface is then accomplished through the Rab11 positive endosomes (Benhra et al, 2011;Benhra et al, 2010;Emery et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Neur promotes Delta endocytosis in the anterior pI daughter cell (Benhra et al, 2010;Le Borgne and Schweisguth, 2003b). The Delta recycling to the apical pIIa/pIIb interface is then accomplished through the Rab11 positive endosomes (Benhra et al, 2011;Benhra et al, 2010;Emery et al, 2005). Among other activities, Delta recycling requires the exocyst complex and the formation of apical actin-rich (ARS) structure at the level of the apical midbody, which forms upon pI cytokinesis (Jafar-Nejad et al, 2005;Langevin et al, 2005b;Rajan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division

et al. 2013

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SUMMARYAsymmetric cell division generates cell fate diversity during development and adult life. Recent findings have demonstrated that during stem cell divisions, the movement of centrosomes is asymmetric in prophase and that such asymmetry participates in mitotic spindle orientation and cell polarization. Here, we have investigated the dynamics of centrosomes during Drosophila sensory organ precursor asymmetric divisions and find that centrosome movements are asymmetric during cytokinesis. We demonstrate that centrosome movements are controlled by the cell fate determinant Numb, which does not act via its classical effectors, Sanpodo and α-Adaptin, but via the Collapsin Response Mediator Protein (CRMP). Furthermore, we find that CRMP is necessary for efficient Notch signalling and that it regulates the duration of the pericentriolar accumulation of Rab11-positive endosomes, through which the Notch ligand, Delta is recycled. Our work characterizes an additional mode of asymmetric centrosome movement during asymmetric divisions and suggests a model whereby the asymmetry in centrosome movements participates in differential Notch activation to regulate cell fate specification.

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Section: Crmp Controls the Duration Of Rab11 Accumulation Around The mentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division

et al. 2013

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“…the mechanosensory bristles) located along the cuticle of the adult Drosophila, and is critically dependent on Notch: during a program of three rounds of asymmetric cell division [177,178], each division generates one daughter cell that assumes the signal-sending role and uses DSLs to activate Notch in its sibling, which acts as a signal-receiving cell [179]. In the signal-sending cells of SOP, Delta localizes both at the apical and at the basolateral membrane, while Notch accumulates apically [180]. By using a pulse chase antibody uptake assay coupled to confocal microscopy sectioning, it was demonstrated that the basolateral pool of Delta is continuously endocytosed and delivered to the apical PM, where the interaction with Notch is most likely to occur [180].…”

Section: Mechanistic Modelsmentioning

confidence: 99%

“…By using a pulse chase antibody uptake assay coupled to confocal microscopy sectioning, it was demonstrated that the basolateral pool of Delta is continuously endocytosed and delivered to the apical PM, where the interaction with Notch is most likely to occur [180]. This observation was extended by the same Authors to highly-polarized mammalian cells: by using a compartmentalized antibody uptake assay, they showed that Dll1 is similarly internalized from the basolateral membrane of Madin-Darby canine kidney cells and then transcytosed to the apical plasma membrane where Notch1 accumulates [180]. In a second set of experiments on SOP the function of two primary regulators of actin dynamics was explored, i.e.…”

Section: Mechanistic Modelsmentioning

confidence: 99%

Endocytosis in Notch Signaling Activation

Sala¹,

Ruggiero²,

Giacomo³

et al. 2012

Molecular Regulation of Endocytosis

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Regulation of stem cell function by protein ubiquitylation

2014

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Tissue homeostasis depends largely on the ability to replenish impaired or aged cells. Thus, tissue-resident stem cells need to provide functional progeny throughout the lifetime of an organism. Significant work in the past years has characterized how stem cells integrate signals from their environment to shape regulatory transcriptional networks and chromatin-regulating factors that control stem cell differentiation or maintenance. There is increasing interest in how post-translational modifications, and specifically ubiquitylation, control these crucial decisions. Ubiquitylation modulates the stability and function of important factors that regulate key processes in stem cell behavior. In this review, we analyze the role of ubiquitylation in embryonic stem cells and different adult multipotent stem cell systems and discuss the underlying mechanisms that control the balance between quiescence, self-renewal, and differentiation. We also discuss deregulated processes of ubiquitin-mediated protein degradation that lead to the development of tumor-initiating cells. Stem cells: concepts and definitionsEmbryonic stem cells and adult tissue-resident stem cells are of great interest in biology and medicine due to their unique characteristics [1]. They have the ability to self-renew, which is defined as the capacity to proliferate while being able to differentiate to downstream cellular types upon proper stimuli from their environment. This unparalleled cellular plasticity of stem cells identifies them as key determinants of tissue equilibrium.Although progenitor cells also have the ability to self-renew, this is usually a short-term characteristic [2]. The long-term self-renewal capacity of stem cells is essential to supply tissues with differentiated progeny throughout the life of the organism. Adult stem cells reside in specialized microenvironments called niches and manifest different degrees of quiescence, depending on the specific organ characteristics. For example, hematopoietic stem cells (HSCs) are dormant [3], whereas mammary stem cells (MaSCs) appear to be cycling [4] and intestinal stem cells (ISCs) proliferate rapidly [5].Stem cells can divide symmetrically or asymmetrically [6; Sidebar A]. Symmetric cell divisions ensure that all elements are distributed equally between the two identical daughter stem cells, and differentiation-usually of only one of the daughter cells-occurs at a later stage. Asymmetric cell divisions, on the other hand, lead to the unequal division of stem cell components in a process that involves proper positioning of the mitotic spindle [7]. As a result, one cell remains a stem cell, whereas the other adopts a different cell fate. Asymmetric divisions also physically displace one daughter cell from its relative position to the niche, leading to its differentiation.Signals from the niche microenvironment are critical in regulating intrinsic stem cell transcriptional programs. Various signaling pathways such as Wnt, Hedgehog, Notch, TGF-b/BMP, and JAK/STAT act in concert to sha...

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Neuralized Promotes Basal to Apical Transcytosis of Delta in Epithelial Cells

Cited by 57 publications

References 40 publications

Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division

Regulation of centrosome movements by Numb and the Collapsin Response Mediator Protein during Drosophila sensory progenitor asymmetric division

Endocytosis in Notch Signaling Activation

Regulation of stem cell function by protein ubiquitylation

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