Neural Transplantation in Spinal Cord under Different Conditions of Lesions and Their Functional Significance

Das, Gopal D.

doi:10.1007/978-1-4612-4846-0_1

Cited by 5 publications

(1 citation statement)

References 121 publications

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“…Glial scar formation is not specific to MS plaques but represents a general response to CNS injury. It has been observed following such diverse types of CNS injury as knife-or laceration-type lesions (Das, 1986(Das, , 1987 and focal irradiation (fast alpha-particle; Maxwell and Kruger, 1965) as well as during Wallerian degeneration of severed nerve fiber tracts (Dahl et al, 1981a). Astrocytes responding to injury become "reactive" : they proliferate (R.H. Miller et al, 1986), become enlarged (Fedoroff et al, 1984), and extend numerous cell processes (Bignami and Dahl, 1976).…”

Section: Introductionmentioning

confidence: 97%

Novel astrocytic protein in multiple sclerosis plaques

Malhotra

Predy

Johnson

et al. 1989

J of Neuroscience Research

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Monoclonal antibody J1-31 (MAb J1-31, isotype IgG 2b) was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Müller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP. Using a model system for gliosis (laceration-type injury of rat spinal cord), we were able to show that astrocytes responding to central nervous system injury exhibit greatly enhanced staining for J1-31 antigen (Predy et al.: Journal of Neuroscience Research 19:397-404, 1988; Predy and Malhotra: Brain Research Bulletin in press, 1989). In this article, we demonstrate that immunofluorescence staining owing to MAb J1-31 is greatly enhanced in MS plaques, as compared to adjacent "apparently normal" white matter. (This is consistent with previous results as MS plaques characteristically show an astroglial response [reactive gliosis] leading to the formation of a glial scar [McKhann: Annual Review of Neuroscience 5:219-239, 1982].) In addition, we present further evidence that J1-31 antigen is distinct from GFAP, although these two proteins may be associated spatially with one another.

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Section: Introductionmentioning

confidence: 97%

Novel astrocytic protein in multiple sclerosis plaques

Malhotra

Predy

Johnson

et al. 1989

J of Neuroscience Research

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Enhanced expression of a protein antigen (JI‐31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord

Predy

Malhotra

Das

1988

J of Neuroscience Research

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A mouse monoclonal antibody (MAb J1-31, isotype IgG 2b) was raised against an autopsy sample of cerebral white matter from a multiple sclerosis (MS) patient. MAb J1-31 recognizes a protein (J1-31 antigen) in human brain which has a molecular weight of approximately 30,000 daltons (30 kD) as determined by immunoprecipitation followed by SDS-gel electrophoresis (reducing conditions) and autoradiography (Singh et al.: Biosci Rep 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains glial fibrillary acidic protein (GFAP)-positive cells, namely astrocytes, of both human and rat. Yet J1-31 antigen is distinct from GFAP (Predy et al.: Biosci Rep 7:491-502, 1987). In this paper we report that greatly enhanced staining for J1-31 antigen is exhibited by reactive astrocytes which arise following CNS injury. (Laceration-type surgical lesion of the rat spinal cord served as the experimental model). Enhanced expression of J1-31 antigen reveals some new aspect of the astrocyte response to injury, involving transformation to the reactive state. Consequently, MAb J1-31 may be a useful marker for studies on reactive astrocytes.

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Fetal Neural Tissue Transplantation for Spinal Cord Injury Repair

Venkatachalam

2012

Human Fetal Tissue Transplantation

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Neural Transplantation in Spinal Cord under Different Conditions of Lesions and Their Functional Significance

Cited by 5 publications

References 121 publications

Novel astrocytic protein in multiple sclerosis plaques

Novel astrocytic protein in multiple sclerosis plaques

Enhanced expression of a protein antigen (JI‐31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord

Fetal Neural Tissue Transplantation for Spinal Cord Injury Repair

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