Neural tract tracing using Di-I: a review and a new method to make fast Di-I faster in human brain

Sparks, D. Larry; Lue, Lih‐Fen; Martin, Timothy A.; Rogers, Joseph

doi:10.1016/s0165-0270(00)00291-0

Cited by 49 publications

(38 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ciliary bodies (3), the lens (4), and the vitreous body (5) also demonstrate a Mn 2ϩ -induced signal enhancement. At the optic chiasm (6), most axons cross to the contralateral side, where the projection continues in the optic tract (7) to the lateral geniculate nucleus (8), pretectal area (9), and superior colliculus (10). Some of the optic tract axons synapse in the lateral geniculate nucleus and post-synaptic axons project in the optic radiation (11).…”

Section: Discussionmentioning

confidence: 99%

Manganese‐enhanced MRI of the optic visual pathway and optic nerve injury in adult rats

Thuen¹,

Singstad

Pedersen³

et al. 2005

Magnetic Resonance Imaging

106

View full text Add to dashboard Cite

Purpose: To evaluate manganese (Mn 2ϩ )-enhanced MRI in a longitudinal study of normal and injured rat visual projections. Materials and Methods:MRI was performed 24 hours after unilateral intravitreal injection of MnCl 2 (150 nmol) into adult Fischer rats that were divided into four groups: 1) controls (N ϭ 5), 2) dose-response (N ϭ 10, 0.2-200 nmol), 3) time-response with repeated MRI during 24 -168 hours post injection (N ϭ 4), and 4) optic nerve crush (ONC) immediately preceding the MnCl 2 injection (N ϭ 7). Control and ONC animals were reinjected with MnCl 2 20 days after the first injection, and MRI was performed 24 hours later. Results:In the control group, the optic projection was visualized from the retina to the superior colliculus, with indications of transsynaptic transport to the cortex. There was a semilogarithmic relationship between the Mn 2ϩ dose and Mn 2ϩ enhancement from 4 to 200 nmol, and the enhancement decayed gradually to 0 by 168 hours. No Mn 2ϩ -enhanced signal was detected distal to the ON crush site. In the control group, similar enhancement was obtained after the first and second MnCl 2 injections, while in the ONC group the enhancement proximal to the crush site was reduced 20 days post lesion (20dpl). Conclusion:Mn 2ϩ -enhanced MRI is a viable method for temporospatial visualization of normal and injured ON in the adult rat. The observed reduction in the Mn 2ϩ signal proximal to the ONC is probably a result of retrograde damage to the retinal ganglion cells, and not of Mn 2ϩ toxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Manganese‐enhanced MRI of the optic visual pathway and optic nerve injury in adult rats

Thuen¹,

Singstad

Pedersen³

et al. 2005

Magnetic Resonance Imaging

106

View full text Add to dashboard Cite

show abstract

“…Extending incubation times beyond 6 weeks appeared to have no consistent effect on the degree of labeling although this parameter can be important for visualizing projections (Lukas et al, 1998;Sparks et al, 2000). Perhaps myelination is more advanced for projections to the contralateral IC than for ipsilaterally projecting axons (e.g., sequential myelination of corpus callosum, Looney and Elberger, 1986; differential myelination of spinal tracts, Hamano et al, 1998) and, thus, interferes with DiI confusion or detection.…”

Section: Labeling and Histologymentioning

confidence: 96%

Development of banded afferent compartments in the inferior colliculus before onset of hearing in ferrets

et al. 2007

View full text Add to dashboard Cite

Axonal projections from the lateral superior olivary nuclei (LSO), as well as from the dorsal cochlear nucleus (DCN) and dorsal nucleus of the lateral lemniscus (DNLL), converge in frequency-ordered layers in the central nucleus of the inferior colliculus (IC) where they distribute among different synaptic compartments. A carbocyanine dye, DiI, was used as a tracer to study the postnatal development of axonal projections in the ferret IC. The results indicated that projections from all three nuclei are present at birth, but are not segregated into bands. During the postnatal week between approximately postnatal days 4 and 12 (P4-P12), axons from LSO proliferate in IC, become more branched, and segregate into a series of bands composed of densely packed fibers and endings. LSO projections in these afferent bands course parallel to IC layers and are separated by intervening regions with few endings. A modest fit of a sine curve (R 2 >0.15) to the pattern of spacing of LSO projections in IC indicated that regularly spaced bands are forming by P7. Similarly, banded patterns of DCN and DNLL projections to IC have developed by the end of the first postnatal week. Thus, well before hearing onset in ferret (P28-30), three different afferent projections have segregated into banded compartments along layers in the central nucleus of the ferret IC. Possible mechanisms in circuit development are discussed.

show abstract

“…The contribution of primary duct cells to SMG morphogenesis was examined using DiI, which is a highly lipophilic fluorescent dye (Sparks et al, 2000). Under the conditions that allowed labeled end bud cells to expand in the ductal region in the presence of FGF1-FGF7 (Fig.…”

Section: Epithelial Wnt Signaling Modulates Fgf-dependent End Bud Difmentioning

confidence: 99%

The Wnt-Myb pathway suppresses KIT expression to control the timing of salivary proacinar differentiation and duct formation

et al. 2016

View full text Add to dashboard Cite

Growth factor signaling is involved in the development of various organs, but how signaling regulates organ morphogenesis and differentiation in a coordinated manner remains to be clarified. Here, we show how WNT signaling controls epithelial morphogenetic changes and differentiation using the salivary gland as a model. Experiments using genetically manipulated mice and organ cultures revealed that WNT signaling at an early stage (E12-E15) of submandibular salivary gland (SMG) development inhibits end bud morphogenesis and differentiation into proacini by suppressing Kit expression through the upregulation of the transcription factor MYB, and concomitantly increasing the expression of distal progenitor markers. In addition, WNT signaling at the early stage of SMG development promoted end bud cell proliferation, leading to duct formation. WNT signaling reduction at a late stage (E16-E18) of SMG development promoted end bud maturation and suppressed duct formation. Thus, WNT signaling controls the timing of SMG organogenesis by keeping end bud cells in an undifferentiated bipotent state.

show abstract

Neural tract tracing using Di-I: a review and a new method to make fast Di-I faster in human brain

Cited by 49 publications

References 44 publications

Manganese‐enhanced MRI of the optic visual pathway and optic nerve injury in adult rats

Manganese‐enhanced MRI of the optic visual pathway and optic nerve injury in adult rats

Development of banded afferent compartments in the inferior colliculus before onset of hearing in ferrets

The Wnt-Myb pathway suppresses KIT expression to control the timing of salivary proacinar differentiation and duct formation

Contact Info

Product

Resources

About