Neural Stem/Progenitor Cells Express 20 Tenascin C Isoforms That Are Differentially Regulated by Pax6

Holst, Alexander von; Egbers, Ursula; Prochiantz, Alain; Faissner, Andréas

doi:10.1074/jbc.m608067200

Cited by 80 publications

(107 citation statements)

References 48 publications

(27 reference statements)

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“…TNC is involved in CNS embryogenesis, especially in the formation of neural stem cell niches [27][28][29]. TNC expression, which is under the control of Oct-4 [62], is low in normal brain tissue and upregulated in several malignant brain tumors in association with increased vascularity, short time to relapse, and poor prognosis [26,63].…”

Section: Discussionmentioning

confidence: 99%

“…Most Oct-4 + cells expressed surface TNC, a marker of neural stem/progenitor cells [28] ( Figure 2B, 1-3), and HIF-2α ( Figure 2B, [5][6][7], that controls Oct-4 expression in embryonic stem cells [40] (Figure 2A), but not HIF-1α [35]. HIF-2α, but not TNC, was expressed also by approximately 65% Oct-4 − tumor cells ( Figure 2B, 5-7 and Figure 2A).…”

Section: Identification and Immunophenotypic Characterization Of Oct-mentioning

confidence: 99%

“…In order to exclude the possibility that detection of surface TNC on NB cells was due to release of soluble TNC from the tumor extracellular matrix and subsequent binding to receptors expressed on malignant cells, we next performed real-time PCR experiments with specific primers detecting all TNC isoforms [28] using mRNA from the TNC + HTLA-230 and TNC − GI-LI-N NB cell lines. As shown in Supplementary information, Figure 2S, TNC mRNA was detected in HTLA-230 but not GI-LI-N cells, indicating that the former cells produced TNC endogenously.…”

Section: Expression Of Tnc Mrna In Human Htla-230 Cell Linementioning

confidence: 99%

“…Tumor-derived TNC promotes or enhances the process of neovascularization in different tumor models [23][24][25][26][27][28]51].…”

Section: Orthotopic Nb Tumors Formed By Tnc + But Not Tnc − Htla-230 mentioning

confidence: 99%

“…Both primary and metastatic NB cells as well as NB cell lines were found to express Oct-4. Tenascin C (TNC), a protein of the extracellular matrix expressed by many tumor cell types and involved in tumor growth, metastasis and angiogenesis [23][24][25][26][27][28][29], was consistently expressed on the surface of most Oct-4 + NB cells but not of bulk tumor cells. The availability of TNC as a surface marker allowed one to positively select Oct-4 + NB cells to perform functional studies proving that Oct-4 + /TNC + cells serve as TEC progenitors.…”

Section: Introductionmentioning

confidence: 99%

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Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells

Pezzolo¹,

Parodi²,

Marimpietri³

et al. 2011

Cell Res

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Section: Discussionmentioning

confidence: 99%

Section: Identification and Immunophenotypic Characterization Of Oct-mentioning

confidence: 99%

Section: Expression Of Tnc Mrna In Human Htla-230 Cell Linementioning

confidence: 99%

“…Tumor-derived TNC promotes or enhances the process of neovascularization in different tumor models [23][24][25][26][27][28]51].…”

Section: Orthotopic Nb Tumors Formed By Tnc + But Not Tnc − Htla-230 mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells

Pezzolo¹,

Parodi²,

Marimpietri³

et al. 2011

Cell Res

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Post‐transcriptional regulation of gene expression in neural stem cells

Kim

2016

Cell Biochemistry & Function

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Expression of each gene can be controlled at several steps during the flow of genetic information from DNA to protein. Tight regulation of gene expression is especially important for stem cells because of their greater ripple effects, compared with terminally differentiated cells. Dysregulation of gene expression arising in stem cells can be perpetuated within the stem cell pool via self-renewal throughout life. In addition, transcript profiles within stem cells can determine the selective advantage or disadvantage of each cell, leading to changes in cell fate, such as a tendency for proliferation, death, and differentiation. The identification of neural stem/progenitor cells (NSPCs) and greater understanding of their cellular physiology have raised the possibility of using NSPCs to replace damaged or injured neurons. However, an accurate grasp of gene expression control must take precedence in order to use NSPCs in therapies for neurological diseases. Recently, accumulating evidence has demonstrated the importance of post-transcriptional regulation in NSPC fate decisions. In this review, we will summarize and discuss the recent findings on key mRNA modulators and their vital roles in NSPC homeostasis. Copyright © 2016 John Wiley & Sons, Ltd.

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Differential expression of phosphacan/RPTPβ isoforms in the developing mouse visual system

Klausmeyer

Garwood

Faissner

2007

J of Comparative Neurology

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The chondroitin sulfate proteoglycan DSD-1-PG/phosphacan represents one of four splice variants of receptor-protein-tyrosine-phosphatase-beta/zeta (RPTPbeta/zeta). This receptor is expressed by glial cells and occurs in two isoforms, RPTPbeta(long) and RPTPbeta(short). The secreted forms phosphacan and phosphacan short isoform (PSI) bind to extracellular matrix and adhesion molecules and might mediate astroglial effects on neuronal differentiation. Phosphacan and RPTPbeta(long) both carry the DSD-1 epitope, a glycosaminoglycan modification that is involved in stimulating neurite outgrowth of embryonic rat mesencephalic and hippocampal neurons in a polycationic environment. Additionally, phosphacan inhibits neurite outgrowth of embryonic DRG neurons in the presence of laminin. In the light of these functional properties we examined the expression patterns of the DSD-1 epitope and phosphacan isoforms in the developing mouse visual system. During retinal development the DSD-1 epitope appears around embryonic day (E)13, peaks around postnatal day (P)6, and is downregulated from P9 to adolescence. By comparison, the phosphacan core protein is first detectable at E12, reaches maximal levels around P14, and persists, although at lower levels, to adulthood. The DSD-1 epitope is restricted to the nerve fiber and the inner plexiform layers. In contrast, the phosphacan core protein immunoreactivity extends from the nerve fiber layer to the outer plexiform layer. The level of expression of the phosphacan/RPTPbeta gene was investigated by reverse-transcriptase polymerase chain reaction. These experiments suggest that there is a shift in the expression patterns of the different phosphacan/RPTPbeta isoforms during late embryonic and postnatal development. In situ hybridization experiments support the conclusion that at least one of the phosphacan/RPTPbeta isoforms in the retina is expressed by neurons.

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Neural Stem/Progenitor Cells Express 20 Tenascin C Isoforms That Are Differentially Regulated by Pax6

Cited by 80 publications

References 48 publications

Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells

Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells

Post‐transcriptional regulation of gene expression in neural stem cells

Differential expression of phosphacan/RPTPβ isoforms in the developing mouse visual system

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