Neural Stem/Progenitor Cell Clones as Models for Neural Development and Transplantation

Li, Hedong; Zhao, He; Shu, Xiaoqiong; Jiang, Mei

doi:10.5772/29532

Cited by 1 publication

(5 citation statements)

References 118 publications

(110 reference statements)

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“…Although v-myc has been regarded as a non-transforming gene and previous transplantation studies using v-myc induced neural progenitors show no signs of tumorigenecity 29 , we did observe cell mass formation after transplantation of GE6 cells especially transplantation of undifferentiated GE6 cells. This correlates with higher v-myc expression in GE6 compared with other clones such as CTX8.…”

Section: Discussioncontrasting

confidence: 78%

“…Alternative immortalizing methods including tetracycline-controllable myc expression system could further increase safety of these cells after transplantation. Although reduced v-myc expression level does not seem to interfere with interneuronal differentiation and maturation 29 30 , residual v-myc level in the mature neuronal cells may affect their physiological functions and potentially tumorigenic in longer term. Therefore, a tighter control on v-myc expression would eliminate these potential problems.…”

Section: Discussionmentioning

confidence: 99%

“…During CNS development, multipotential neural stem cells become diversified in a spatiotemporal fashion and progressively restricted in their choices of cell types that they can give rise to. These cell-fate-restricted progenitors maintain proliferation ability and generate specific neural cell types in a precisely controlled manner before exhausted at late stages of development 28 29 . Our success in isolating a variety of neural progenitors from different regions of the E14.5 rat forebrain by v-myc immortalization strongly supports the presence of these diversified neural progenitors 30 .…”

Section: Discussionmentioning

confidence: 99%

“…Along with other genes, v-myc has been used as an immortalizing gene for decades, and v-myc transduced NSC clones have been reported previously 29 . However, GABAergic interneuronal progenitor clones by v-myc have not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, alternative sources of these cells are clearly needed. Generation of neural stem cell (NSC) clones by Myc-transduction has been developed decades ago, and therapeutical potentials of these clones have been extensively demonstrated 28 29 .…”

mentioning

confidence: 99%

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An interneuron progenitor maintains neurogenic potential in vivo and differentiates into GABAergic interneurons after transplantation in the postnatal rat brain

Wang

Hong

Gao

et al. 2016

Sci Rep

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Dysfunction of cortical GABAergic interneurons are involved in numerous neurological disorders including epilepsy, schizophrenia and autism; and replenishment of these cells by transplantation strategy has proven to be a feasible and effective method to help revert the symptoms in several animal models. To develop methodology of generating transplantable GABAergic interneurons for therapy, we previously reported the isolation of a v-myc-induced GABAergic interneuron progenitor clone GE6 from embryonic ganglionic eminence (GE). These cells can proliferate and form functional inhibitory synapses in culture. Here, we tested their differentiation behavior in vivo by transplanting them into the postnatal rat forebrain. We found that GE6 cells migrate extensively in the neonatal forebrain and differentiate into both neurons and glia, but preferentially into neurons when compared with a sister progenitor clone CTX8. The neurogenic potential of GE6 cells is also maintained after transplantation into a non-permissive environment such as adult cortex or when treated with inflammatory cytokine in culture. The GE6-derived neurons were able to mature in vivo as GABAergic interneurons expressing GABAergic, not glutamatergic, presynaptic puncta. Finally, we propose that v-myc-induced human interneuron progenitor clones could be an alternative cell source of transplantable GABAergic interneurons for treating related neurological diseases in future clinic.

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Section: Discussioncontrasting

confidence: 78%

Section: Discussionmentioning

confidence: 99%