Neural stem cells differentiation ability of human umbilical cord mesenchymal stromal cells is not altered by cryopreservation

Zhang, Hongtian; Hong, Chen; Zhao, Hao; Dai, Yiwu; Xu, Ran

doi:10.1016/j.neulet.2010.10.008

Cited by 17 publications

(15 citation statements)

References 15 publications

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“…★: compared to NH 2 group on day 2; ◆: compared to NH 2 group on day 4; ☆: compared to NH 2 group on day 6. (n = 5) functions.…”

Section: Discussionmentioning

confidence: 99%

“…Neural stem cells (NSCs) or neuronal progenitor cells with the ability to regenerate functional neural cells have recently drawn more and more attention because of research interests and clinical applications [4][5][6][7]. In vivo, NSCs and neuronal progenitor cells are in an environment that supplies them with repertoire soluble and solid-phase components that regulate cell behavior and function [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Various fates of neuronal progenitor cells observed on several different chemical functional groups

Liu

Wang

et al. 2011

Front. Mater. Sci.

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Neuronal progenitor cells cultured on gold-coated glass surfaces modified by different chemical functional groups, including hydroxyl ( -OH), carboxyl ( -COOH), amino ( -NH 2 ), bromo ( -Br), mercapto ( -SH), -Phenyl and methyl ( -CH 3 ), were studied here to investigate the influence of surface chemistry on the cells' adhesion, morphology, proliferation and functional gene expression. Focal adhesion staining indicated in the initial culture stage cells exhibited morphological changes in response to different chemical functional groups. Cells cultured on -NH 2 grafted surface displayed focal adhesion plaque and flattened morphology and had the largest contact area. However, their counter parts on -CH 3 grafted surface displayed no focal adhesion and rounded morphology and had the smallest contact area. After 6 days culture, the proliferation trend was as follows: -NH 2 > -SH > -COOH > -Phenyl > -Br > -OH > -CH 3 . To determine the neural functional properties of the cells affected by surface chemistry, the expression of glutamate decarboxylase (GAD67), nerve growth factor (NGF) and brainderived neurotrophic factor (BDNF) were characterized. An increase of GAD67 expression was observed on -NH 2 , -COOH and -SH grafted surfaces, while no increase in NGF and BDNF expression was observed on any chemical surfaces. These results highlight the importance of surface chemistry in the fate determination of neuronal progenitor cells, and suggest that surface chemistry must be considered in the design of biomaterials for neural tissue engineering.

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“…★: compared to NH 2 group on day 2; ◆: compared to NH 2 group on day 4; ☆: compared to NH 2 group on day 6. (n = 5) functions.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Various fates of neuronal progenitor cells observed on several different chemical functional groups

Liu

Wang

et al. 2011

Front. Mater. Sci.

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“…59,60 It has been reported that there were no morphological changes to MSCs after cryopreservation. 9,53,61 MSCs are positive for mesenchymal-associated markers such as CD90, CD105, and CD73, while they lack hematopoietic associated markers such as CD34, CD19 CD45, CD14, and HLA DRDPDQ. 62,63 After cryopreservation, MSCs retained a similar expression level of positive and negative CD markers.…”

Section: Cell Phenotype and Proliferation Capacitymentioning

confidence: 99%

“…62,63 After cryopreservation, MSCs retained a similar expression level of positive and negative CD markers. 7,50,[53][54][55]61 Overall, existing evidence shows that cryopreservation has no significant effect on phenotype of MSCs.…”

Section: Cell Phenotype and Proliferation Capacitymentioning

confidence: 99%

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

Yong

Zaman

Xufeng

et al. 2015

Biopreservation and Biobanking

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Mesenchymal stem cells (MSCs) hold many advantages over embryonic stem cells (ESCs) and other somatic cells in clinical applications. MSCs are multipotent cells with strong immunosuppressive properties. They can be harvested from various locations in the human body (e.g., bone marrow and adipose tissues). Cryopreservation represents an efficient method for the preservation and pooling of MSCs, to obtain the cell counts required for clinical applications, such as cell-based therapies and regenerative medicine. Upon cryopreservation, it is important to preserve MSCs functional properties including immunomodulatory properties and multilineage differentiation ability. Further, a biosafety evaluation of cryopreserved MSCs is essential prior to their clinical applications. However, the existing cryopreservation methods for MSCs are associated with notable limitations, leading to a need for new or improved methods to be established for a more efficient application of cryopreserved MSCs in stem cell-based therapies. We review the important parameters for cryopreservation of MSCs and the existing cryopreservation methods for MSCs. Further, we also discuss the challenges to be addressed in order to preserve MSCs effectively for clinical applications.

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“…UCB derived MSCs show high morphological and molecular similarities to bone marrow derived MSC including the lack of hematopoietic surface antigens [8]. Furthermore, several authors have differentiated UCB-MSCs in vitro to osteogenic, chondrogenic, neural and hepatic lineages successfully [9][10][11]. On the other hand, the isolation of MSCs from UCB has been shown to be very difficult because of their low number, compared to BM.…”

Section: Introductionmentioning

confidence: 99%

LNGFR (CD271) as Marker to Identify Mesenchymal Stem Cells from Different Human Sources: Umbilical Cord Blood, Wharton?s Jelly and Bone Marrow

Maria¹,

Yolanda²,

Angel³

2013

J Bone Marrow Res

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Bone marrow has been considered the main source for isolation of multipotent mesenchymal stem cells for therapeutic approaches. However, alternative sources of mesenchymal stem cells have been sought because their frequency and differentiating capacity decrease with age and because they are not easy to obtain from healthy donors. These cells have been also described both in umbilical cord blood and in Wharton's jelly. To date, several studies have shown that CD271 is the most specific marker expressed of bone marrow mesenchymal stem cells before being cultured. The aim of this study was to determine whether it is possible to identify not cultured mesenchymal stem cells umbilical cord blood and Wharton's Jelly using this marker, as well as to compare the results found to those obtained in bone marrow.Bone marrow, umbilical cord blood and Wharton's Jelly cells suspension were analyzed by flow cytometry in order to identify the CD271 + /CD45-population. Furthermore, we evaluated the potential to obtain mesenchymal stem cells from each source. Our results showed CD271 + /CD45-cells in bone marrow samples, although this population did not appeared in umbilical cord blood nor from Wharton's Jelly. However, the absence of this phenotype before culture samples had no relation with the ability to culture mesenchymal stem cells, specially taking into account that is was possible to obtain them from Wharton's Jelly but not from umbilical cord blood. Our findings suggest that CD271 is not an adequate marker to identify mesenchymal stem cells from human umbilical cord before culture.

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Neural stem cells differentiation ability of human umbilical cord mesenchymal stromal cells is not altered by cryopreservation

Cited by 17 publications

References 15 publications

Various fates of neuronal progenitor cells observed on several different chemical functional groups

Various fates of neuronal progenitor cells observed on several different chemical functional groups

Cryopreservation of Human Mesenchymal Stem Cells for Clinical Applications: Current Methods and Challenges

LNGFR (CD271) as Marker to Identify Mesenchymal Stem Cells from Different Human Sources: Umbilical Cord Blood, Wharton?s Jelly and Bone Marrow

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