Neural Stem Cells Derived Directly from Adipose Tissue

Petersen, Eric D.; Zenchak, Jessica R.; Lossia, Olivia V.; Hochgeschwender, Ute

doi:10.1089/scd.2017.0195

Cited by 14 publications

(10 citation statements)

References 30 publications

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“…However, as we all know, the unfavorable accessibility combined with ethical issues of human NSCs have long restricted their wide clinical use. This problem could be potentially solved if we could develop an easy way to obtain NSC/neural progenitor cells (NPCs) from more accessible stem cell sources [19][20][21][22] , such as ADSCs.…”

Section: Introductionmentioning

confidence: 99%

Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells

Peng

et al. 2019

Cell Transplant

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Human adipose-derived stem cells are used in regenerative medicine for treating various diseases including osteoarthritis, degenerative arthritis, cartilage or tendon injury, etc. However, their use in neurological disorders is limited, probably due to the lack of a quick and efficient induction method of transforming these cells into neural stem or progenitor cells. In this study, we reported a highly efficient and simple method to induce adipose-derived stem cells into neural progenitor cells within 12 hours, using serum-free culture combined with a well-defined induction medium (epidermal growth factor 20 ng/ml and basic fibroblast growth factor, both at 20 ng/ml, with N2 and B27 supplements). These adipose-derived stem cell-derived neural progenitor cells grow as neurospheres, can self-renew to form secondary neurospheres, and can be induced to become neurons and glial cells. Real-time polymerase chain reaction showed significantly upregulated expression of neurogenic genes Sox2 and Nestin with a moderate increase in stemness gene expression. Raybio human growth factor analysis showed a significantly upregulated expression of multiple neurogenic and angiogenic cytokines such as brain-derived neurotrophic factor, glial cell line-derived neurotrophic growth factor, nerve growth factor, basic fibroblast growth factor and vascular endothelial growth factor etc. Therefore, adipose-derived stem cell-derived neurospheres can be a new source of neural progenitor cells and hold great potential for future cell replacement therapy for treatment of various refractory neurological diseases.

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Section: Introductionmentioning

confidence: 99%

Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells

Peng

et al. 2019

Cell Transplant

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“…The amount described is much higher than the used in this study, which was only 2 × 10 4 cells/mL. Although the sources of formation were different, the present study does not use growth factors, and the number of cells necessary to produce neurospheres was lower [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the present study used conventional media and no supplementation in the culture medium to produce neurospheres from undifferentiated MSCs, regardless of MSC-WJ or MSC-UCB, only the NFBX was coating the culture flasks before hMSCs plating was conducted. The NFBX method made this study more economical, practical, and without the drawbacks of inductions [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Human Mesenchymal Stem Cells Seeded on the Natural Membrane to Neurospheres for Cholinergic-like Neurons

Stricker¹,

Dobuchak²,

Irioda³

et al. 2021

Membranes

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This study aimed to differentiate human mesenchymal stem cells (hMSCs) from the human umbilical cord in cholinergic-like neurons using a natural membrane. The isolation of hMSCs from Wharton’s jelly (WJ) was carried out using “explant” and mononuclear cells by the density gradient from umbilical blood and characterized by flow cytometry. hMSCs were seeded in a natural functional biopolymer membrane to produce neurospheres. RT-PCR was performed on hMSCs and neurospheres derived from the umbilical cord. Neural precursor cells were subjected to a standard cholinergic-like neuron differentiation protocol. Dissociated neurospheres, neural precursor cells, and cholinergic-like neurons were characterized by immunocytochemistry. hMSCs were CD73+, CD90+, CD105+, CD34- and CD45- and demonstrated the trilineage differentiation. Neurospheres and their isolated cells were nestin-positive and expressed NESTIN, MAP2, ßIII-TUBULIN, GFAP genes. Neural precursor cells that were differentiated in cholinergic-like neurons expressed ßIII-TUBULIN protein and choline acetyltransferase enzyme. hMSCs seeded on the natural membrane can differentiate into neurospheres, obtaining neural precursor cells without growth factors or gene transfection before cholinergic phenotype differentiation.

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“…In vivo experiments showed that adipose-derived stem cells could act as a possible source of brain progenitor cells and that they are clinically important in neurological applications (36). Moreover, their efficiency can be improved by the addition of growth factors to media formulations, as this would enhance the efficiency of differentiation of adipose tissue into neural stem cells (37).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Adipose-Derived Mesenchymal Stem Cells into Neuron-Like Cells induced by using β-mercaptoethanol

et al. 2020

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Background: Adipose derived-mesenchymal stem cells have been used as an alternative to bone marrow cells in this study. Objective: We investigated the in vitro isolation, identification, and differentiation of stem cells into neuron cells, in order to produce neuron cells via cell culture, which would be useful in nerve injury treatment. Method: Mouse adipose mesenchymal stem cells were dissected from the abdominal subcutaneous region. Neural differentiation was induced using β-mercaptoethanol. This study included two different neural stage markers, i.e. nestin and neurofilament light-chain, to detect immature and mature neurons, respectively. Results: The immunocytochemistry results showed that the use of β-mercaptoethanol resulted in the successful production of neuron cells. This was attributable to the increase and significant overexpression of the nestin protein during the early exposure period, which resulted in the expression of the highest levels of nestin. In comparison, the expression level of neurofilament light-chain protein also increased with time but less than nestin. Non-treated mesenchymal stem cells, considered as control showed very low expression for both markers. Conclusion: The results of this study indicate that adipose mesenchymal cells represent a good, easily obtainable source of bone marrow cells used to developing the differentiation process.

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Neural Stem Cells Derived Directly from Adipose Tissue

Cited by 14 publications

References 30 publications

Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells

Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells

Human Mesenchymal Stem Cells Seeded on the Natural Membrane to Neurospheres for Cholinergic-like Neurons

Differentiation of Adipose-Derived Mesenchymal Stem Cells into Neuron-Like Cells induced by using β-mercaptoethanol

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