Neural Stem Cells

Galli, Rossella; Gritti, Angela; Bonfanti, Luca; Vescovi, Angelo L.

doi:10.1161/01.res.0000065580.02404.f4

Cited by 226 publications

(128 citation statements)

References 101 publications

(131 reference statements)

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“…MuStem cells were able to expand in suspension, an experimental condition that does not support proliferation of differentiated cells that rapidly die. 69 In this original proliferation context, MuStem cells gave rise to large clusters of rounded cells termed myospheres, which have been also described for cells freshly isolated from mice 41 and human 70 skeletal muscle.…”

Section: Discussionmentioning

confidence: 92%

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Rouger

Larcher

Dubreil

et al. 2011

The American Journal of Pathology

100

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Duchenne muscular dystrophy (DMD) is a genetic progressive muscle disease resulting from the lack of dystrophin and without effective treatment. Adult stem cell populations have given new impetus to cellbased therapy of neuromuscular diseases. One of them, muscle-derived stem cells, isolated based on delayed adhesion properties, contributes to injured muscle repair. However, these data were collected in dystrophic mice that exhibit a relatively mild tissue phenotype and clinical features of DMD patients. Here, we characterized canine delayed adherent stem cells and investigated the efficacy of their systemic delivery in the clinically relevant DMD animal model to assess potential therapeutic application in humans. Delayed adherent stem cells, named MuStem cells (muscle stem cells), were isolated from healthy dog muscle using a preplating technique. In vitro, MuStem cells displayed a large expansion capacity, an ability to proliferate in suspension, and a multilineage differentiation potential. Phenotypically, they corresponded to early myogenic progenitors and uncommitted cells. When injected in immunosuppressed dystrophic dogs, they contributed to myofiber regeneration, satellite cell replenishment, and dystrophin expression. Importantly, their systemic delivery resulted in long-term dystrophin expression, muscle damage course limitation with an increased regeneration activity and an interstitial expansion restriction, and persisting stabilization of the dog's clinical status. These results demonstrate that MuStem cells could provide an attractive therapeutic avenue for DMD patients.

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Section: Discussionmentioning

confidence: 92%

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Rouger

Larcher

Dubreil

et al. 2011

The American Journal of Pathology

100

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“…Relatively quiescent neural stem cells and fast-proliferating multipotential progenitors residing in the adult SVZ are characterized by the ability to self-renew and generate distinct cell types (Galli et al, 2003;Alvarez-Buylla and Lim, 2004), thus providing an important therapeutic tool (Picard-Riera et al, 2004). Given their ability to proliferate, self-renew, and migrate, these cells have also the potential to undergo neoplastic transformation (Recht et al, 2003), and a large body of evidence supports this concept, although the identity of the transformed cells is not well defined (Rao, 1999;Holland, 2000;Berger et al, 2004;Jang et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Loss ofp53Induces Changes in the Behavior of Subventricular Zone Cells: Implication for the Genesis of Glial Tumors

Gil-Perotín¹,

Marin-Husstege²,

Li³

et al. 2006

J. Neurosci.

196

182

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The role of multipotential progenitors and neural stem cells in the adult subventricular zone (SVZ) as cell-of-origin of glioblastoma has been suggested by studies on human tumors and transgenic mice. However, it is still unknown whether glial tumors are generated by all of the heterogeneous SVZ cell types or only by specific subpopulations of cells. It has been proposed that transformation could result from lack of apoptosis and increased self-renewal, but the definition of the properties leading to neoplastic transformation of SVZ cells are still elusive. This studyaddressesthesequestionsinmicecarryingthedeletionofp53,atumor-suppressorgeneexpressedintheSVZ.Weshowherethat,although loss of p53 by itself is not sufficient for tumor formation, it provides a proliferative advantage to the slow-and fast-proliferating subventricular zone (SVZ) populations associated with their rapid differentiation. This results in areas of increased cell density that are distributed along the walls of the lateral ventricles and often associated with increased p53-independent apoptosis. Transformation occurs when loss of p53 is associated with a mutagenic stimulus and is characterized by dramatic changes in the properties of the quiescent adult SVZ cells, including enhanced self-renewal, recruitment to the fast-proliferating compartment, and impaired differentiation.Together, these findings provide a cellular mechanism for how the slow-proliferating SVZ cells can give rise to glial tumors in the adult brain.

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“…4 Thus, in adult mammalian brains, vascular cells are close to CNS germinal zones throughout life, and NSCs mainly localize in the dentate gyrus of the hippocampus, subventricular zone, and olfactory bulb. 5 Therefore, dense areas of microvessels distribute in these regions. We speculate that microvessels can produce a special microenvironment that may influence the bioactivity of NSCs.…”

mentioning

confidence: 99%

Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells

Guo

Shi

et al. 2007

Microsurgery

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In adult mammalian brain, vascular cells reside throughout life, close to central nervous system germinal zones, and neural stem cells (NSCs) mainly localize in the dentate gyrus of the hippocampus, subventricular zone, and olfactory bulb. Microvessels appear to produce a special microenvironment that may influence the characteristics of NSCs. To explore this potential correlation, an in vitro model with cocultured cerebral microvascular endothelial cells (CMECs) and NSCs was established in our study by using a transwell coculture system.

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Neural Stem Cells

Cited by 226 publications

References 101 publications

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Systemic Delivery of Allogenic Muscle Stem Cells Induces Long-Term Muscle Repair and Clinical Efficacy in Duchenne Muscular Dystrophy Dogs

Loss ofp53Induces Changes in the Behavior of Subventricular Zone Cells: Implication for the Genesis of Glial Tumors

Proliferation and neurogenesis of neural stem cells enhanced by cerebral microvascular endothelial cells

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