Neural pathways in the pallial nerve and arm nerve cord revealed by neurobiotin backfilling in the cephalopod mollusk Octopus vulgaris

Imperadore, Pamela; Lepore, María Grazia; Ponte, Giovanna; Pflüger, Hans‐Joachim; Fiorito, Graziano

doi:10.1007/s10158-019-0225-y

Cited by 11 publications

(8 citation statements)

References 44 publications

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“…[57][58][59][60][61] The brain imaging results presented here form a bridge to the past work of Young and others where classical histology was used and more recently to those also using antibody labeling. 11,15,24,48,57,[62][63][64][65][66] We chose a comparative approach and investigated four species of octopodiform cephalopods that represent phylogenetically distinct groups and that exhibit different life modes, including the deep-sea Vampyroteuthis infernalis, a solitary nocturnal Hapalochlaena fasciata, and two diurnal reef dwellers, Abdopus capricornicus and O. cyanea (Figure 1). In addition to these newly described species, another four coastal octopus species were selected from published literature 11,15,17,18 and included for further analyses where comparative data exist (Table S2).…”

Section: Introductionmentioning

confidence: 99%

Comparative brain structure and visual processing in octopus from different habitats

2022

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Section: Introductionmentioning

confidence: 99%

Comparative brain structure and visual processing in octopus from different habitats

2022

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“…Pallial nerves together with stellate ganglia and surrounding tissues were harvested from sacrificed animals for neural tracing experiments. Backfill protocol was performed following Imperadore et al (Imperadore et al, 2019). In brief, the far end of the pallial nerve was placed in a Vaseline pool filled with tracer solution (Neurobiotin 5% in distilled water, Vector Laboratories Cat #SP-1120, RRID: AB_2313575) while the remaining tissue was immersed in sea water.…”

Section: Methodsmentioning

confidence: 99%

From injury to full repair: nerve regeneration and functional recovery in the common octopus,Octopus vulgaris

Imperadore

Parazzoli

Oldani

et al. 2019

Preprint

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Spontaneous nerve regeneration in cephalopod molluscs occurs in a relative short time after injury, achieving functional recovery of the lost capacities. In particular, transection of the pallial nerve in the common octopus (Octopus vulgaris) determines loss and subsequent restoring of two functions fundamental for survival, i.e. breathing and skin patterning, the latter involved in communication between animals and concealing. The phenomena occurring after lesion have been investigated in a series of previous studies, but a complete analysis of the changes occurring at the level of the axons and the effects on animals appearance during the whole regenerative process is still missing. Our goal is to determine the course of events following injury. Our goal is to determine the course of events following injury, from impairment to full recovery.We observed nerve regeneration, end-target re-innervation and functional reconnections between central brain and periphery, using the contralateral nerve in the same animal as internal control. The final architecture of the regenerated nervous tissue does not mirror the original structure, however functionality returns to match the phenotype of an intact octopus, and with no visible impact on the behaviour of the animal. This provides exceptional value to these findings for future studies.Summary statementHere we report events occurring after interruption of the peripheral neural circuitry in Octopus vulgaris, from the dramatic loss of normal functioning to full recovery.

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“…Pallial nerves together with stellate ganglia and surrounding tissues were harvested from animals after death for neural tracing experiments. The backfill protocol was performed following Imperadore et al (2019). In brief, the far end of the pallial nerve was placed in a Vaseline pool filled with tracer solution (Neurobiotin, 5% in distilled water, Vector Laboratories SP-1120, ID RRID:AB_2313575) while the remaining tissue was immersed in seawater.…”

Section: Tissue Sampling and Backfillingmentioning

confidence: 99%

From injury to full repair: nerve regeneration and functional recovery in the common octopus, Octopus vulgaris

Imperadore

Parazzoli

Oldani

et al. 2019

Journal of Experimental Biology

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Spontaneous nerve regeneration in cephalopod molluscs occurs in a relative short time after injury, achieving functional recovery of lost capacity. In particular, transection of the pallial nerve in the common octopus (Octopus vulgaris) determines the loss and subsequent restoration of two functions fundamental for survival, i.e. breathing and skin patterning, the latter involved in communication between animals and concealment. The phenomena occurring after lesion have been investigated in a series of previous studies, but a complete analysis of the changes taking place at the level of the axons and the effects on the animals' appearance during the whole regenerative process is still missing. Our goal was to determine the course of events following injury, from impairment to full recovery. Through imaging of the traced damaged nerves, we were able to characterize the pathways followed by fibres during regeneration and end-target re-innervation, while electrophysiology and behavioural observations highlighted the regaining of functional connections between the central brain and periphery, using the contralateral nerve in the same animal as an internal control. The final architecture of a fully regenerated pallial nerve does not exactly mirror the original structure; however, functionality returns to match the phenotype of an intact octopus with no observable impact on the behaviour of the animal. Our findings provide new important scenarios for the study of regeneration in cephalopods and highlight the octopus pallial nerve as a valuable 'model' among invertebrates.

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Neural pathways in the pallial nerve and arm nerve cord revealed by neurobiotin backfilling in the cephalopod mollusk Octopus vulgaris

Cited by 11 publications

References 44 publications

Comparative brain structure and visual processing in octopus from different habitats

Comparative brain structure and visual processing in octopus from different habitats

From injury to full repair: nerve regeneration and functional recovery in the common octopus,Octopus vulgaris

From injury to full repair: nerve regeneration and functional recovery in the common octopus, Octopus vulgaris

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