“…Since then, a series of methods, such as colorimetric analysis, spectroscopic techniques (e.g., visible absorption spectroscopy, infrared spectroscopy, Raman spectroscopy, hyperspectral imaging, , electron paramagnetic resonance spectroscopy, etc. ), and electrochemical methods , have been adopted for the investigation of the Hb degradation. ,− Nevertheless, the Hb ex vivo oxidation mechanism is complex, leading to the inaccuracy of the estimation results. The Hb ex vivo oxidation process can also lead to structural changes in red blood cells such as volume, surface area, perimeter, and height, as well as the nanomechanical features (e.g., the viscoelasticity), which have been explored by atomic force microscopy (AFM) .…”