Neural modeling of bromelain extraction by reversed micelles

Fileti, Ana Maria Frattini; Fischer, Gilvan Anderson; Tambourgi, Elias Basile

doi:10.1590/s1516-89132010000200026

Cited by 20 publications

(15 citation statements)

References 6 publications

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“…In addition, the reverse micelle solvent system is another ATPE that can be used as an alternative technique for protein separation due to its simplicity and feasibility of large-scale sample loading [94]. The reverse micelle solvent system is a very attractive system for protein separation [95,96]. Lastly, there is an application of a novel continuous ATPE prototype for the recovery of biomolecules [97].…”

Section: Recent Process In Atpe Applications Economic Costsmentioning

confidence: 99%

Aqueous Two-Phase Extraction Advances for Bioseparation

Goja¹,

Yang

Cui³

et al. 2013

J Bioproces Biotechniq

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Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of solute from one aqueous phase to another. ATPE includes polymer-polymer type and polymer-salt type systems for the recovery of proteins. The protein must be recovered in a highly purified form in order to improve its quality, decrease energy consumption, reduce waste and minimize costs. To acquire the high value and achieve good control over processes, the reliable, multi-component products are required especially those with the ability to investigate complex processing conditions. The current reviewing paper discusses the most recent progresses for the recovery of biomolecules by using the ATPE, covering the mechanism, which controls the phase formation and the behavior of solute partitioning in aqueous twophase systems (ATPS) processes. The review discusses also the increasing application for the recovery of high-value bioproducts, the recent development of alternative low cost ATPS and disadvantages attributed to ATPS.

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Section: Recent Process In Atpe Applications Economic Costsmentioning

confidence: 99%

Aqueous Two-Phase Extraction Advances for Bioseparation

Goja¹,

Yang

Cui³

et al. 2013

J Bioproces Biotechniq

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“…The raw extract was obtained from the leaf and stem of pineapples in vitro using 1g mass which was macerated in 5 mL of 0.2M phosphate buffer at different pH (5.7, 6.7 and 7.7) and filtered twice in gauze (Almeida et al, 2002;Ferreira et al 2011;Fileti et al 2009 and2010;Lopes 2005;Lopes et al 2009 and. Total protein was determined according to Bradford's method (Bradford 1976;Ferreira et al 2007;Padilha et al 2012;Severo Júnior et al 2007) and enzymatic activity by the casein digestion method, or Kunitz's method, with modifications Lopes 2005;Lopes et al 2009;Silveira et al 2009).…”

Section: Obtaining the Enzymementioning

confidence: 99%

“…The fruit is also a good option for its source as it has higher proteolytic activity than in the stem. A good quantity of fruit residue is generated by the pine apple processing factories, which can be used as raw material (Ferreira et al 2011;Fileti et al 2009 and2010;Lopes et al 2012;Parks 2001). In vitro cultivation is an important procedure in the multiplication of different species, This technique can be applied on the industrial scale.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the study on both purification and alternative extraction methods is of great interest, aiming to obtain more economically feasible processes. Hence, solid-liquid extraction applied to a technological process is extremely important for the development of new protein extraction and purification methods (Ferreira et al 2011;Fileti et al 2009 and2010;Lopes et al 2009;Silveira et al 2009). This study aimed to quantify bromelain activity in different parts of pineapple plants produced in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Leite

Lima

Santana

et al. 2012

Braz. arch. biol. technol.

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This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola's

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“…It was first introduced in 1957 (Kelly 1996) for acute inflammation and sports injury. This enzyme has broad-spectrum therapeutic efficacy proven in vitro and in vivo [ 20 ] [ 21 ] [ 23 ] [ 24 ] [ 25 ] [ 26 ] [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

The Alteration of Plasma Matrix Metalloproteinase-9 Level after the Addition of Bromelin 500 mg to Standard Therapy of Acute Ischemic Stroke and Its Correlation with Outcome

Sinurat¹,

Sjahrir²,

Rambe³

et al. 2018

Open Access Maced J Med Sci

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BACKGROUND:Matrix metalloproteinase-9 (MMP9) expression due to ischemic cause spreading of brain damage. Previous studies have reported that Bromelin was beneficial as anti-inflammation and prevent brain tissue damage.AIM:This study aimed to determine the alteration of plasma MMP9 level after addition of Bromelin 500 mg to Standard therapy and its correlation with outcome in acute ischemic stroke.METHODS:This was a preliminary report of a prospective randomised, double-blind study with pre and post-test design, forty-six acute ischemic stroke patients were randomly allocated with Bromelin and Standard groups. Measurement of MMP9 and outcome were performed before and after 14-days treatment.RESULT:The Bromelin group showed a significant decrement of MMP9 level, from 6.02 ± 0.32 ng/ml before treatment to 5.50 ± 0.94 ng/ml after treatment (p = 0.028). There was a negative correlation between MMP9 level and mRS (r= -0.03; p = 0.905) and a positive correlation toward BI (r = 0.039; p = 0.859), while the Standard group showed increased MMP9 level from 5.82 ± 0.71 ng/ml to 5.91 ± 0.83 ng/ml (p = 0.616) which was correlated insignificantly to outcome.CONCLUSION:We concluded that the addition of 500 mg Bromelin to standard ischemic stroke therapy reduced MMP9 level significantly and correlated to outcome improvement. However, there is a tight statistical correlation.

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Neural modeling of bromelain extraction by reversed micelles

Cited by 20 publications

References 6 publications

Aqueous Two-Phase Extraction Advances for Bioseparation

Aqueous Two-Phase Extraction Advances for Bioseparation

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

The Alteration of Plasma Matrix Metalloproteinase-9 Level after the Addition of Bromelin 500 mg to Standard Therapy of Acute Ischemic Stroke and Its Correlation with Outcome

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