Neural mechanism of acute stress regulation by trace aminergic signalling in the lateral habenula in male mice

Yang, Soo Hyun; Yang, Esther; Lee, Jaekwang; Kim, Jin Yong; Yoo, Hyeijung; Park, Hyung Sun; Jung, Jin Taek; Lee, Dongmin; Chun, Sungkun; Jo, Yong Sang; Pyeon, Gyeong Hee; Park, Jae Yong; Lee, Hyun Woo; Kim, H.

doi:10.1038/s41467-023-38180-7

Cited by 5 publications

(2 citation statements)

References 65 publications

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“…Laser parameters were set up as follows: magnification 5×, power 56, aperture 9, speed 3, specimen balance 17, pulse frequency 501. The anatomical identification of the RMTg region was according to the earlier study [ 29 , 101 ], and tissue was collected, placed in a 0.5 mL Eppendorf tube, and then stored at −80 ℃ until RNA extraction.…”

Section: Methodsmentioning

confidence: 99%

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice

Fu,

Li,

Mai

et al. 2024

IJMS

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Alcohol use disorders (AUDs) frequently co-occur with negative mood disorders, such as anxiety and depression, exacerbating relapse through dopaminergic dysfunction. Stress-related neuropeptides play a crucial role in AUD pathophysiology by modulating dopamine (DA) function. The rostromedial tegmental nucleus (RMTg), which inhibits midbrain dopamine neurons and signals aversion, has been shown to increase ethanol consumption and negative emotional states during abstinence. Despite some stress-related neuropeptides acting through the RMTg to affect addiction behaviors, their specific roles in alcohol-induced contexts remain underexplored. This study utilized an intermittent voluntary drinking model in mice to induce negative effect behavior 24 h into ethanol (EtOH) abstinence (post-EtOH). It examined changes in pro-stress (Pnoc, Oxt, Npy) and anti-stress (Crf, Pomc, Avp, Orx, Pdyn) neuropeptide-coding genes and analyzed their correlations with aversive behaviors. We observed that adult male C57BL/6J mice displayed evident anxiety, anhedonia, and depression-like symptoms at 24 h post-EtOH. The laser-capture microdissection technique, coupled with or without retrograde tracing, was used to harvest total ventral tegmental area (VTA)-projecting neurons or the intact RMTg area. The findings revealed that post-EtOH consistently reduced Pnoc and Orx levels while elevating Crf levels in these neuronal populations. Notably, RMTg Pnoc and Npy levels counteracted ethanol consumption and depression severity, while Crf levels were indicative of the mice’s anxiety levels. Together, these results underscore the potential role of stress-related neuropeptides in the RMTg in regulating the negative emotions related to AUDs, offering novel insights for future research.

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Section: Methodsmentioning

confidence: 99%

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice

Fu,

Li,

Mai

et al. 2024

IJMS

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“…Chronic stress drives depression via the excitation of the LHb glutamatergic neurons, thereby enhancing the RMTg GABAergic projection to the VTA and leading to the hypoactivity of VTA dopamine neurons [152]. However, part of LHb glutamatergic neurons express aromatic L-amino acid decarboxylase, called D-neurons; acute stress can decrease the excitability of these D-neurons and reduce RMTg GABAergic inputs to the VTA via trace aminergic signaling, leading to the activation of VTA dopamine neurons [153]. The biphasic roles of VTA dopaminergic neurons under stress conditions were found in different stress-induced mouse models.…”

Section: Vta and Lateral Habenulamentioning

confidence: 99%

The Formation and Function of the VTA Dopamine System

Hou,

Hao,

Duan

et al. 2024

IJMS

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The midbrain dopamine system is a sophisticated hub that integrates diverse inputs to control multiple physiological functions, including locomotion, motivation, cognition, reward, as well as maternal and reproductive behaviors. Dopamine is a neurotransmitter that binds to G-protein-coupled receptors. Dopamine also works together with other neurotransmitters and various neuropeptides to maintain the balance of synaptic functions. The dysfunction of the dopamine system leads to several conditions, including Parkinson’s disease, Huntington’s disease, major depression, schizophrenia, and drug addiction. The ventral tegmental area (VTA) has been identified as an important relay nucleus that modulates homeostatic plasticity in the midbrain dopamine system. Due to the complexity of synaptic transmissions and input–output connections in the VTA, the structure and function of this crucial brain region are still not fully understood. In this review article, we mainly focus on the cell types, neurotransmitters, neuropeptides, ion channels, receptors, and neural circuits of the VTA dopamine system, with the hope of obtaining new insight into the formation and function of this vital brain region.

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A nociceptive amygdala-striatal pathway for chronic pain aversion

Wojick,

Paranjapye,

Chiu

et al. 2024

Preprint

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The basolateral amygdala (BLA) is essential for assigning positive or negative valence to sensory stimuli. Noxious stimuli that cause pain are encoded by an ensemble of nociceptive BLA projection neurons (BLAnociensemble). However, the role of the BLAnociensemble in mediating behavior changes and the molecular signatures and downstream targets distinguishing this ensemble remain poorly understood. Here, we show that the same BLAnociensemble neurons are required for both acute and chronic neuropathic pain behavior. Using single nucleus RNA-sequencing, we characterized the effect of acute and chronic pain on the BLA and identified enrichment for genes with known functions in axonal and synaptic organization and pain perception. We thus examined the brain-wide targets of the BLAnociensemble and uncovered a previously undescribed nociceptive hotspot of the nucleus accumbens shell (NAcSh) that mirrors the stability and specificity of the BLAnociensemble and is recruited in chronic pain. Notably, BLAnociensemble axons transmit acute and neuropathic nociceptive information to the NAcSh, highlighting this nociceptive amygdala-striatal circuit as a unique pathway for affective-motivational responses across pain states.

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Neural mechanism of acute stress regulation by trace aminergic signalling in the lateral habenula in male mice

Cited by 5 publications

References 65 publications

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice

Association between RMTg Neuropeptide Genes and Negative Effect during Alcohol Withdrawal in Mice

The Formation and Function of the VTA Dopamine System

A nociceptive amygdala-striatal pathway for chronic pain aversion

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