Neural influences on synovial mast cell density in rat

Levine, Jon D.; Coderre, Terence J.; Covinsky, Ken; Basbaum, Allan I.

doi:10.1002/jnr.490260306

Cited by 30 publications

(13 citation statements)

References 46 publications

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“…Although such proximity might occur at random, in vitro co-culture studies have shown the formation of specific contacts between rat sympathetic neurons and mast cell (Blennerhassett and Bienenstock 1990;Blennerhassett et al 1991), and chemical sympathectomy produced by guanethidine treatment reduced the synovial mast cells density in vivo (Levine et al 1990). Functional studies indicate a bilateral interaction between mast cells and sympathetic axons.…”

Section: Dermal Blood Vesselsmentioning

confidence: 99%

A quantitative ultrastructural investigation of tyrosine hydroxylase-immunoreactive axons in the hairy skin of the guinea pig

Roth

Kummer

1994

Anat Embryol

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Besides its thermoregulatory role, the sympathetic innervation of the skin is involved in a modulation of sensory processing and trophic functions that has not been fully characterized. To investigate possible sites at which such sympathosensory interactions might occur, a quantitative ultrastructural study of the sympathetic innervation of the skin was attempted. The hairy skin of the guinea pig was studied because the sympathetic and sensory nerve axons in this species can easily be discriminated by the presence of immunoreactivity to the catecholamine-synthesizing enzyme, tyrosine hydroxylase (TH). The thermoregulatory role of the sympathetic skin innervation was highlighted by the almost exclusive sympathetic innervation of piloarrector muscles which contained 62% (n = 195) of randomly selected TH-immunoreactive (TH-IR) axon profiles. Of TH-IR pilomotor axons, 53% were filled with vesicles. Vesicle-containing axonal profiles were equally frequent around dermal arterial blood vessels (partly associated with mast cells), hair follicles, and within nerve fibre bundles surrounded by a perineural sheath, in each case accounting for about 3% of all dermal TH-IR axonal profiles. In contrast to piloarrector muscles, at these locations TH-IR (sympathetic) and non-reactive (sensory) axons were found in close association. These findings are in line with the previously reported inhibitory influence of sympathetic stimulation upon hair follicle afferents and perivascular sensory nerve terminals. In addition, they point to a yet underestimated target of sympathetic axon terminals, i.e. preterminal nerve fibre bundles.

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Section: Dermal Blood Vesselsmentioning

confidence: 99%

A quantitative ultrastructural investigation of tyrosine hydroxylase-immunoreactive axons in the hairy skin of the guinea pig

Roth

Kummer

1994

Anat Embryol

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“…The patients suffer from frequency of urination and pain which is relieved by voiding. It has been shown recently that the innervation in the synovial joint of the rat, by unmyelinated afferent and sympathetic efferent fibres, exerts a tropic effect on mast cell density (Levine et al, 1990). The classic form of IC (Hunner's ulcer) is characterised by a high density of mast cells in the bladder wall (Aldenborg et al, 1986).…”

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confidence: 99%

Interstitial Cystitis: Correlation with Nerve Fibres, Mast Cells and Histamine

Lundeberg

Liedberg

Nordling

et al. 1993

British Journal of Urology

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Interstitial cystitis (IC) is a painful condition in the urinary bladder of unknown aetiology and pathogenesis. To assess the contribution of the nervous system to IC a biopsy was taken from 6 patients with IC and from a control group of 6 patients with non-ulcerative IC. Mast cells were counted and histamine measured in bladder washings from all patients. There were significantly more nerve fibres within the sub-urothelium and detrusor muscle in chronic IC than there were in non-ulcerative IC. The bladder washings from all patients with IC contained mast cells and histamine, while only occasional mast cells and traces of histamine were found in washings from patients with non-ulcerative IC. There was a good correlation between the number of nerve fibres and number of mast cells as well as between the number of nerve fibres and the amount of histamine.

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“…However, it is also possible that the vitamin D deficient diet may have resulted in mast cell degranulation, which reduced the number of resident neurturin-ir mast cells. While we cannot rule out the possibility that the reduction in mast cells occurs secondary to the loss of innervation, as has been previously suggested (Levine et al, 1990a), the most straightforward explanation is that non-peptidergic axons were reduced in VD- rats in response to a decrease in neurturin-synthesizing mast cells.…”

Section: 0 Discussionmentioning

confidence: 67%

“…Mouse anti-CD163 was used at a dilution of 1:500 (AbD Serotec (Damm et al, 2011)). Mast cells are also present within the synovium where they are intimately associated with nerves, and their density has been correlated with synovial innervation density (Levine et al, 1990a, Hukkanen et al, 1991). Mouse anti-mast cell tryptase (MCT) diluted 1:250 (Abcam (Walls et al, 1990)) was used as a mast cell marker.…”

Section: Methodsmentioning

confidence: 99%

Vitamin D deficiency leads to sensory and sympathetic denervation of the rat synovium

Tague

Smith

2014

Neuroscience

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Vitamin D deficiency is associated with increased susceptibility to inflammatory arthritis. Sensory and sympathetic synovial nerves are critical to the development of inflammatory arthritis and spontaneously degenerate in the early phases of disease. These nerves contain vitamin D receptors and vitamin D influences nerve growth and neurotrophin expression. We therefore examined the density of synovial nerves and neurotrophin-containing cells in vitamin D deficient rats. Seven week old Sprague Dawley rats were fed either control or vitamin D deficient diets for four weeks. Knee synovium sections extending from patella to meniscus were immunostained for total nerves, myelinated and unmyelinated nerves, sympathetic nerves, peptidergic and non-peptidergic sensory nerves, and neurotrophins and immune cell markers. In control rats, intimal innervation by unmyelinated sensory fibers was denser than subintimal innervation. In contrast, sympathetic innervation was confined to the subintima. Many sensory axons contained markers for both peptidergic and non-peptidergic nerves. NGF was primarily expressed by intimal CD163-negative type B synoviocytes, while neurturin, a ligand selective for non-peptidergic sensory neurons, was expressed by synovial mast cells. In vitamin D deficient rats, there were significant reductions in sensory nerves in the intima and sympathetic nerves in the subintima. While there was no significant change in NGF-immunoreactivity, the number of neurturin-expressing mast cells was significantly reduced in the intima, suggesting that intimal reductions in sensory nerves may be related to reductions in neurturin. Vitamin D deficiency therefore may increase susceptibility to inflammatory arthritis by depleting sensory and sympathetic synovial nerves as a result of reduced synovial neurotrophin content.

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Neural influences on synovial mast cell density in rat

Cited by 30 publications

References 46 publications

A quantitative ultrastructural investigation of tyrosine hydroxylase-immunoreactive axons in the hairy skin of the guinea pig

A quantitative ultrastructural investigation of tyrosine hydroxylase-immunoreactive axons in the hairy skin of the guinea pig

Interstitial Cystitis: Correlation with Nerve Fibres, Mast Cells and Histamine

Vitamin D deficiency leads to sensory and sympathetic denervation of the rat synovium

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