Neural Ganglia Transcriptome and Peptidome Associated with Sexual Maturation in Female Pacific Abalone (Haliotis discus hannai)

Kim, Mi Ae; Markkandan, Kesavan; Han, Nayoung; Park, Jong; Lee, Jung Sick; Lee, Hookeun; Sohn, Young Chang

doi:10.3390/genes10040268

Cited by 21 publications

(30 citation statements)

References 77 publications

(117 reference statements)

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“…Several recent studies have identified a number of reproductive and metabolic genes in H. discus hannai (Mendoza-Porras et al 2014;Kim et al 2017). Additionally, neuropeptide precursors that influence sexual maturation in female Pacific abalone have been recently reported by Kim et al (2019). In the present study, neuropeptide transcripts were identified and characterized from the pleuropedal and cerebral ganglia of H. discus hannai to provide valuable resources for future neuroendocrine research in abalone species.…”

Section: Introductionmentioning

confidence: 79%

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Sharker

Sukhan

Kim

et al. 2020

The European Zoological Journal

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Neuropeptides play a central role in the regulation of reproduction, growth, development, and various other physiological processes. In our study, 26 neuropeptide precursors were identified from the pleuropedal and cerebral ganglia of Pacific abalone using transcriptome analysis. Of them, two (neuromacin and neuroglian) were potentially novel and two (myomodulin and FMRFamide) were involved in the reproductive regulation of mollusks. A BLAST search indicated that most of these neuropeptides exhibited the highest homologies with neuropeptides of other protostomian species. Myomodulin, FMRFamide, and molluscan insulin-related peptides shared 87%, 72%, and 88% sequence identities with homologous peptides of the tropical gastropod mollusk, Haliotis asinina. In silico analysis revealed that these identified neuropeptide precursors were likely to be extracellular secreted proteins. Alignment of multiple cerebrin, FFamide, and insulin-related peptide sequences illustrated that most of their biologically active residues were highly conserved with other invertebrate homologous neuropeptide residues. Three-dimensional (3D) structures of adipokinetic hormone, allatostain (A, B, and C), allatotropin, cerebrin, clionin, conopressin, elevenin, and neuromycin precursors in H. discus hannai exhibited a helix-loophelix structure. Based on phylogenetic analysis, buccalin A1 and A2, allatostain B, and FCAP neuropeptide precursors of Pacific abalone formed a clade with other gastropod and bivalve mollusks neuropeptide precursors. This study provides a novel insight for further functional studies of abalone and other gastropod mollusks.

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Section: Introductionmentioning

confidence: 79%

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Sharker

Sukhan

Kim

et al. 2020

The European Zoological Journal

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“…While Idgf4 does not have chitinase activity, it likely promotes cell growth and development, the function of which is unclear in the post-mitotic adult brain 59 . Idgf4 expression was positively correlated with sexual maturation in the neural ganglia of the female Pacific abalone (Haliotis discus hannai) 60 , implying that it has broad maturation-related functionality in different tissue types, including neural.…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis of honey bee neurogenomic response links Deformed wing virus type A to precocious behavioral maturation

Traniello

Bukhari

Kevill

et al. 2020

Sci Rep

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crop pollination by the western honey bee Apis mellifera is vital to agriculture but threatened by alarmingly high levels of colony mortality, especially in Europe and North America. Colony loss is due, in part, to the high viral loads of Deformed wing virus (DWV), transmitted by the ectoparasitic mite Varroa destructor, especially throughout the overwintering period of a honey bee colony. Covert DWV infection is commonplace and has been causally linked to precocious foraging, which itself has been linked to colony loss. taking advantage of four brain transcriptome studies that unexpectedly revealed evidence of covert DWV-A infection, we set out to explore whether this effect is due to DWV-A mimicking naturally occurring changes in brain gene expression that are associated with behavioral maturation. Consistent with this hypothesis, we found that brain gene expression profiles of DWV-A infected bees resembled those of foragers, even in individuals that were much younger than typical foragers. In addition, brain transcriptional regulatory network analysis revealed a positive association between DWV-A infection and transcription factors previously associated with honey bee foraging behavior. Surprisingly, single-cell RNA-Sequencing implicated glia, not neurons, in this effect; there are relatively few glial cells in the insect brain and they are rarely associated with behavioral plasticity. Covert DWV-A infection also has been linked to impaired learning, which together with precocious foraging can lead to increased occurrence of infected bees from one colony mistakenly entering another colony, especially under crowded modern apiary conditions. These findings provide new insights into the mechanisms by which DWV-A affects honey bee health and colony survival. Western honey bees (Apis mellifera) provide an essential pollination service for modern agriculture, with a value estimated at $15 billion per year in the United States 1,2. Extensive reliance on pollination by honey bees has led to great concerns over the massive losses of managed colonies that have occurred since 2006 3-5. These losses are widely thought to be caused by parasites, pathogens, pesticides and poor nutrition, and the main parasite is the ectoparasitic mite Varroa destructor 6-10. Varroa mites are the primary vectors of Deformed wing virus (DWV) 11,12 , a single-stranded, positive-sense RNA virus (family Iflaviridae) that specifically impacts arthropods 13-16. DWV is globally distributed, driving an economic and ecological crisis in honey bee populations 17. DWV was first discovered in symptomatic deformed winged honey bees from Japan in the early 1980s 18 , and the first full genome was sequenced in 2006 19 , hereafter referred to as type A or DWV-A 20. Martin et al. showed through screening the Hawaiian honey bee population that diverse DWV variants persisted prior to the arrival of Varroa 21 ; however, the establishment of Varroa selected for a single variant, DWV-A. DWV-A is currently the most prevalent variant in the North America 22 , thoug...

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“…We found that the peak expression levels of NPF in H. discus hannai and H. gigantea occurred at ZT8 (20:00 h), which was 4 h earlier than the feeding behavior peak, possibly because of the role of NPF in facilitating the feeding behavior of abalone. The amino acid sequence of the neuropeptide group of NPF in H. discus hannai has been determined (Kim et al, 2019). Thus, in future studies, the role of NPF in facilitating the feeding behavior of H. discus hannai could be validated by directly injecting Hdh-NPF.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of the Feeding Characteristics and Digestion Physiology of Haliotis discus hannai and Haliotis gigantea

et al. 2021

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Abalone (Haliotis spp.) are typical nocturnal creatures but Haliotis discus hannai is bold and active in the nighttime whereas H. gigantea tends to be timid and inactive. In this study, we quantified and compared differences in movement, feeding, and digestive physiology between H. discus hannai and H. gigantea as well as the potential molecular mechanisms on the basis of video observations and expression levels of genes related to feeding regulation. The feeding behaviors of both species were characterized by significant circadian rhythms (P < 0.05). However, the distance moved and the cumulative duration of movement were 2.61 and 1.94 times higher, respectively, in H. discus hannai than in H. gigantea over the 24-h cycle. The cumulative duration of feeding by H. discus hannai was only 1.15 times that by H. gigantea, but the feeding time as a percentage of the cumulative duration of movement (FTP) was up to 94.6% for H. gigantea and only 56.0% for H. discus hannai. The peaks for α-amylase activity and NPF expression levels in both species as well as the peak OX2R expression level in H. gigantea occurred during 20:00–00:00 h. By contrast, the peaks for alginate lyase activity and NPYR expression levels in H. discus hannai occurred at 16:00 h, when the FTP was significantly higher for H. discus hannai than for H. gigantea. These initial findings quantify specific behavior parameters and thus provide a reference for the selection of appropriate feeding strategies and the proliferation of abalone via bottom sowing.

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Neural Ganglia Transcriptome and Peptidome Associated with Sexual Maturation in Female Pacific Abalone (Haliotis discus hannai)

Cited by 21 publications

References 77 publications

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Meta-analysis of honey bee neurogenomic response links Deformed wing virus type A to precocious behavioral maturation

Comparative Study of the Feeding Characteristics and Digestion Physiology of Haliotis discus hannai and Haliotis gigantea

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