Neural differentiation of rat muscle-derived stem cells in the presence of valproic acid: A preliminary study

Abstract

In this study, we chose valproic acid (VA) for inducing neural differentiation in rat muscle-derived stem cells (rMDSCs). After pre-induction with basic fibroblast growth factor (bFGF) and subsequent treatment with VA, VA-related cytotoxicity to rMDSCs was relatively low. In addition, VA-treated rMDSCs exhibited a neural-like morphology after 1, 4, and 7 days. The immunocytochemical results of VA-treated rMDSCs showed abundant cells stained positive for neuron-specific enolase (NSE) and neuron filament (NF) as neural markers, oligodendrocyte transcription factor 2 (Olig-2) as an oligodendrocyte marker, and glial fibrillary acidic protein (GFAP) as an astrocyte marker. Our results suggest that rMDSCs can be differentiated into a neural-like phenotype by using VA.

Chitosan-based hydrogels to induce neuronal differentiation of rat muscle-derived stem cells

“…Our previous data indicated that higher concentrations (4 mM) resulted in considerable cell death, and the cytotoxicity induced by VA appeared to be dose-dependent [18]. Thus, VA at a concentration of 1 mM, which was only slightly toxic, was selected to represent a differentiation of rMDSCs in the subsequent experiments in this work.…”

“…VA-induction medium was prepared at 0 and 1 mM VA. Concentration dependence of VA in rMDSCs on TCP was performed in previous work [18]. Cytotoxicity was measured using a WST-1 kit (Roche, Germany) after 1, 4, and 7 days according to the manufacturer's instructions.…”

“…Although previous studies have demonstrated the neural differentiation of MDSCs in the presence of VA [15,18], these studies typically showed low cell survival. However, several 3-dimensional scaffolds with geometric or topographical features may provide a suitable environment for the survival, expansion, and modulation of the self-renewal and differentiation of adult stem cells [19,20].…”

Intrinsic Ability of Adult Stem Cell in Skeletal Muscle: An Effective and Replenishable Resource to the Establishment of Pluripotent Stem Cells

“…Functional linkage between skeletal muscle and the nervous system at neuromuscular junction is necessary for the normal function of various organs. Although a number of methods have been explored, muscle-derived stem cells can be induced to adopt neuronal lineages [148–152]. Arsic et al reported that muscle-derived stem cells (skeletal muscle) began to express N-CAM, β -tubulin III, and GFAP [153].…”

Gene Transfection of Human Turbinate Mesenchymal Stromal Cells Derived from Human Inferior Turbinate Tissues

“…One suggested disadvantage of ASCs is the limitation of the types of stem cells into which they can differentiate. However, this issue has also been overcome following recent studies demonstrating the potential of stem cell transdifferentiation to extraneous cells with their origin tissues [ 8 – 10 ]. The most commonly used ASCs for regenerative medicine are mesenchymal stem cells (MSCs), especially MSCs derived from bone marrow (BMSCs), because of their characteristics such as ease of isolation and fast proliferation in vitro .…”