Neural differentiation of human induced pluripotent stem cells on polycaprolactone/gelatin bi-electrospun nanofibers

Karbalaeimahdi, Ali; Shahrousvand, Mohsen; Javadi, Hamidreza; Ghollasi, Marzieh; Norouz, Faezeh; Kamali, Mohammad; Salimi, Ali

doi:10.1016/j.msec.2017.04.083

Cited by 56 publications

(28 citation statements)

References 44 publications

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“…In a literature review conducted by Mondal et al, it was found that various studies have utilized PCL nanofiberous scaffold to investigate differentiation into different cell lineages such as cardiomyocyte, neuron, myoblast and etc. (Kim et al, 2010;Chen et al, 2015;Mondal et al;KarbalaeiMahdi et al, 2017). Although Eslahi et al demonstrated proliferation of fresh and frozen-thawed mSSCs on PLLA nanofibrous scaffold, there is no study that has demonstrated the differentiation of these cells on such scaffolds.…”

Section: Discussionmentioning

confidence: 99%

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

et al. 2019

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Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.

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Section: Discussionmentioning

confidence: 99%

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

et al. 2019

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“…Moreover, it enhanced adhesion, growth, and proliferation of these cells. KarbalaeiMahdi et al [257] produced gelatin/PCL nanofibers and they showed that their porosity was above 50%. The authors also proved that these biomaterials possessed great mechanical properties (E close to 100 MPa).…”

Section: Nerve Tementioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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“…The application of cellulous nanocrystals has been investigated for a wide range of polymers for the production of nanocomposites . Poly‐(ɛ‐caprolactone) is one of the most attractive polymers for biomedical applications especially in tissue engineering, drug delivery, foams, and composites due to its attractive physical and mechanical properties .…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of reinforced poly (ε‐caprolactone) nanocomposites by cellulose nanowhiskers

2019

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In the present work, cellulose nanowhiskers (CNWs) were extracted from cellulose microfibers powder (CMP) and employed as reinforcement in poly-(ε-caprolactone) (PCL). The biocompatible and biodegradable PCL/CNW nanocomposites reinforced by CNWs in various weight percentages of 0, 0.1, 0.5, and 1 wt% were prepared via solvent casting. The morphology of cellulose microfibers and CNWs were evaluated via the optical microscopy, scanning electron microscopy, and transmission electron microscopy. The mechanical, thermal, and rheological properties of PCL/CNWs film nanocomposites were then investigated. The biodegradability and hydrophilicity of PCL/CNWs were improved by increasing the CNWs content. By adding higher loadings of CNWs, the nanocomposite samples showed higher tensile moduli because of the reinforcing effect of CNWs. The linear viscoelastic properties of PCL was not significantly changed with different loadings of CNWs. K E Y W O R D Scellulose microfibers powder (CMP), cellulose nanowhisker (CNWs), film nanocomposite, poly (ε-caprolactone), viscoelastic measurements

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Neural differentiation of human induced pluripotent stem cells on polycaprolactone/gelatin bi-electrospun nanofibers

Cited by 56 publications

References 44 publications

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Preparation and characterization of reinforced poly (ε‐caprolactone) nanocomposites by cellulose nanowhiskers

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