Neural differentiation of bone marrow mesenchymal stem cells with human brain‐derived neurotrophic factor gene‐modified in functionalized self‐assembling peptide hydrogel in vitro

Luo, Huimin; Xu, Changsheng; Liu, Zhiwei; Yang, Lin; Hong, Yunda; Liu, Guisheng; Zhong, Huifen; Cai, Xinyi; Xu-Ping, Lin; Chen, Xiaokun; Wang, Changsheng; Zhang, Nanwen; Xu, Weili

doi:10.1002/jcb.26408

Cited by 39 publications

(33 citation statements)

References 25 publications

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“…As the derivation of NSCs from ESCs is related to ethical issues, using iPSCs or MSCs as a starting point seems to be an important alternative. In vitro differentiation of MSCs towards NSCs was confirmed for MSCs derived from bone marrow [40,56,66,[77][78][79], adipose tissue [72,[80][81][82][83], umbilical cord blood [67], Wharton's jelly of umbilical cord [61,62,69], and amniotic fluid [84]. In this study, hWJ-MSCs with high neurogenic potential [28,61,65] and the ability to differentiate in vitro towards NSCs [61,62,64,65,68,70] were used to generate hWJ-NSCs.…”

Section: Discussionmentioning

confidence: 71%

“…In contrast to monolayer culture, the generation of a homogenous population of NSCs or NPCs in 3D culture with floating neurospheres is challenging due to the heterogeneity of neurosphere structures. However, 3D cultures maintained on alginian scaffolds [117] or in hydrogels [79] can bring breakthroughs in the field of neural differentiation in the future.…”

Section: Discussionmentioning

confidence: 99%

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Differentiation of Human Mesenchymal Stem Cells from Wharton’s Jelly Towards Neural Stem Cells Using a Feasible and Repeatable Protocol

Kruminis-Kaszkiel

Osowski

Bejer-Oleńska

et al. 2020

Cells

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The transplantation of neural stem cells (NSCs) capable of regenerating to the cells of the central nervous system (CNS) is a promising strategy in the treatment of CNS diseases and injury. As previous studies have highlighted mesenchymal stem cells (MSCs) as a source of NSCs, this study aimed to develop a feasible, efficient, and reproducible method for the neural induction of MSCs isolated from Wharton's jelly (hWJ-MSCs). We induced neural differentiation in a monolayer culture using epidermal growth factor, basic fibroblast growth factor, N2, and B27 supplements. This resulted in a homogenous population of proliferating cells that expressed certain neural markers at both the protein and mRNA levels. Flow cytometry and immunocytochemistry confirmed the expression of neural markers: nestin, sex-determining region Y (SRY) box 1 and 2 (SOX1 and SOX2), microtubule-associated protein 2 (MAP2), and glial fibrillary acidic protein (GFAP). The qRT-PCR analysis revealed significantly enhanced expression of nestin and MAP2 in differentiated cells. This study confirms that it is possible to generate NSCs-like cells from hWJ-MSCs in a 2D culture using a practical method. However, the therapeutic effectiveness of such differentiated cells should be extended to confirm the terminal differentiation ability and electrophysiological properties of neurons derived from them.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Differentiation of Human Mesenchymal Stem Cells from Wharton’s Jelly Towards Neural Stem Cells Using a Feasible and Repeatable Protocol

Kruminis-Kaszkiel

Osowski

Bejer-Oleńska

et al. 2020

Cells

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“…BM-MSCs showed anti-inflammatory effects by promoting the differentiation of lymphocytes. Additionally, BM-MSCs are able to release growth factors such as VEGF, BDNF, GDNF, NGF, epidermal growth factor (EGF), fibroblast growth factor (FGF), and neurotrophin-3 (NT-3), in this way leading on the repair of the lesioned area [37]. Several preclinical studies showed that BM-MSCs release trophic factors into the damaged sites, that inhibit apoptosis, promote angiogenesis and stimulate host progenitor cells to differentiate toward neurons and astrocytes.…”

Section: Stem Cells and Traumatic Brain Injurymentioning

confidence: 99%

Traumatic Brain Injury and Stem Cells: An Overview of Clinical Trials, the Current Treatments and Future Therapeutic Approaches

et al. 2020

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Traumatic brain injury represents physical damage to the brain tissue that induces transitory or permanent neurological disabilities. The traumatic injury activates an important inflammatory response, followed by a cascade of events that lead to neuronal loss and further brain damage. Maintaining proper ventilation, a normal level of oxygenation, and adequate blood pressure are the main therapeutic strategies performed after injury. Surgery is often necessary for patients with more serious injuries. However, to date, there are no therapies that completely resolve the brain damage suffered following the trauma. Stem cells, due to their capacity to differentiate into neuronal cells and through releasing neurotrophic factors, seem to be a valid strategy to use in the treatment of traumatic brain injury. The purpose of this review is to provide an overview of clinical trials, aimed to evaluate the use of stem cell-based therapy in traumatic brain injury. These studies aim to assess the safety and efficacy of stem cells in this disease. The results available so far are few; therefore, future studies need in order to evaluate the safety and efficacy of stem cell transplantation in traumatic brain injury.

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“…Fmoc-F 2 /S [42] pericyte chondrogenesis RADA-PRG (modified PuraMatrix w ) [43,44] mouse neonatal epidermal cells, rMSC neurogenesis RGD on decellularized pig heart valves [45] EPC proliferation Synthemax w [46] iPSC expansion and differentiation polymer enzymatically responsive PEG [47 -52] iPSC, hESC, mESC, MSC, organoids, mouse pancreatic progenitor gellan gum [53] hPSC 3D spheres methylcellulose [54] MSC chondrogenesis nanotopographically imprinted PCL [55,56] MSC maintenance and osteogenesis NO-releasing chitosan [57] hP-MSC angiogenic potential P(PEGMEMA-r-GMA-r-VDM) [58] MSC proliferation PEG with RGD and MMP tethering [59,60] MSC migration photodegradable PEG [61,62] MSC 'mechanical memory' polyacrylate/acrylamide thermoresponsive hydrogel [63 -65] hESC, MSC, mESC polyacrylate/polyurethane [66 -68] hESC, MSC, C6 rat GSC polypyrrole [69] MSC osteogenesis polystyrene TopoChip [70] iPSC proliferation polyurethane [71 -74] hESC-derived HE, HPC, iPSC-derived hepatocytes, H9 ternary polymer blends [75,76] STRO-1 þ skeletal SC, fetal skeletal SC rstb.royalsocietypublishing.org Phil. Trans.…”

Section: Protein-based Substrates For Stem Cell Controlmentioning

confidence: 99%

New substrates for stem cell control

Schmidt¹,

Lilienkampf²,

Bradley³

2018

Phil. Trans. R. Soc. B

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The capacity to culture stem cells in a controllable, robust and scalable manner is necessary in order to develop successful strategies for the generation of cellular and tissue platforms for drug screening, toxicity testing, tissue engineering and regenerative medicine. Creating substrates that support the expansion, maintenance or directional differentiation of stem cells would greatly aid these efforts. Optimally, the substrates used should be chemically defined and synthetically scalable, allowing growth under defined, serum-free culture conditions. To achieve this, the chemical and physical attributes of the substrates should mimic the natural tissue environment and allow control of their biological properties. Herein, recent advances in the development of materials to study/manipulate stem cells, both and are described with a focus on the novelty of the substrates' properties, and on application of substrates to direct stem cells.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.

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Neural differentiation of bone marrow mesenchymal stem cells with human brain‐derived neurotrophic factor gene‐modified in functionalized self‐assembling peptide hydrogel in vitro

Abstract: Bone marrow mesenchymal stem cells can be induced into neural cells by the human brain-derived neurotrophic factor gene in a RADA16-PRG functionalized self-assembling peptide hydrogel. This article is protected by copyright. All rights reserved.

Cited by 39 publications

References 25 publications

Differentiation of Human Mesenchymal Stem Cells from Wharton’s Jelly Towards Neural Stem Cells Using a Feasible and Repeatable Protocol

Differentiation of Human Mesenchymal Stem Cells from Wharton’s Jelly Towards Neural Stem Cells Using a Feasible and Repeatable Protocol

Traumatic Brain Injury and Stem Cells: An Overview of Clinical Trials, the Current Treatments and Future Therapeutic Approaches

New substrates for stem cell control

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