Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice

Autologous transplants of Adipose-Derived Adult Stromal (ADAS) cells afford dopaminergic neuroprotection in a model of Parkinson's disease

“…As indicated in Table 1, mRNA expression of several of these protein markers was confirmed by real-time quantitative PCR analysis (data not shown). These findings are in agreement with reports made for adipose-derived stromal cells from humans, rodents, and non-human primates (Safford, Hicok et al 2002;Zuk, Zhu et al 2002;Ashjian, Elbarbary et al 2003;Safford, Safford et al 2004;Fujimura, Ogawa et al 2005;Romanov, Darevskaya et al 2005;Ning, Lin et al 2006). …”

“…Several studies to date have reported that Adipose-Derived Adult Stromal (ADAS) cells from rats (Tholpady, Katz et al 2003;Yang, Liu et al 2004;Ning, Lin et al 2006), mice (Safford, Hicok et al 2002;Fujimura, Ogawa et al 2005), rhesus monkeys (Kang, Putnam et al 2004), and humans (Safford, Hicok et al 2002;Zuk, Zhu et al 2002;Ashjian, Elbarbary et al 2003;Kang, Jun et al 2003;Fujimura, Ogawa et al 2005) can be coaxed to differentiate into neuronlike morphologies and to express neuro-glial markers in vitro. Importantly, our study is the first to demonstrate that in vitro neural induction and stable terminal differentiation of ADAS cells into functionally mature neurons in vivo are not necessary for ADAS cells to exert neuroprotective effects in models of neurological injury and thus be considered viable tissue sources to treat neurodegenerative diseases.…”

“…In recent years, adult adipose was shown by several laboratories (reviewed in (Schaffler and Buchler 2007) to be a source of multipotent cells from which to derive progenitors such as chondrocytes, adipocytes, osteoblasts, and myocytes for tissue engineering and repair of mesodermal or mesenchymal-derived tissues (Zuk, Zhu et al 2001;Zuk, Zhu et al 2002;Tholpady, Katz et al 2003) while others reported adipose may also have sub-populations of cells with neurogenic potential in vitro (Safford, Hicok et al 2002;Ashjian, Elbarbary et al 2003;Kang, Putnam et al 2004;Safford, Safford et al 2004;Fujimura, Ogawa et al 2005;Ning, Lin et al 2006). The term Adipose-Derived Adult Stem (ADAS) cells was the original term used to refer to these cells on the basis of their potential for multi-lineage specification (Safford, Hicok et al 2002).…”

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

“…Both populations presented identical cell size, doubling times and karyotype stability, differing only in morphology with rounder cells in the sub-odontoblastic compartment versus spindle-shaped cells with long processes in the perivascular one [239]. Pre-clinical experiments have demonstrated that stem cells show promising results in differentiation into neuronal-like cells [226,228,232,233,240] and their secretion of growth factors [229,241,242]. Shi and colleagues [243] stated the potential of glia-derived neurotrophic factor expressing neural stem cells in the regeneration of facial nerve gap in rats.…”

“…Other sources of stem cells have been used in peripheral nerve injuries, such as adipose-derived stem cells [203,206,212,[226][227][228][229][230][231] and dental pulp stem cells (DPSCs) [232][233][234][235][236]. Dental pulp stem cells have also demonstrated differentiation capacity towards multiple other mesodermal and endodermal lineages, under appropriate conditions: adipogenic, osteo/dentinogenic, chondrogenic, neurogenic, endothelial, myofibroblastic [237] and hepatocytes [238].…”