Neural crest cell migration in relation to extracellular matrix organization in the embryonic axolotl trunk

From the Crest to the Periphery: Control of Pigment Cell Migration and Lineage Segregation

“…First, neural crest migration into the dorsolateral space is delayed in chick and mice by more than 24 hr compared with neural crest cells that take the ventral path into the somites. This is different from the timing in axolotl, in which pigment cell migration proceeds at the same rate or even in advance of neural crest cells taking the ventral pathway (Lofberg et al, 1980). Second, pigment cells invade the dorsolateral path as a uniform front over the dorsal surface of the somite, which is again in contrast to the ventrally migrating chick or murine neural crest cells, which only invade the anterior half of each somite (Rickmann et al, 1985;Bronner-Fraser, 1986; Loring and Erickson, 1987; Teillet et al, 1987;Serbedzija et al, 1989; .…”

Embryonic fibroblast motility and orientation can be influenced by physiological electric fields.

“…Third, orientation of the cell axis can be influenced by contact guidance (45, 10) of aligned substratum material such as collagen. It is possible that aligned collagen in the embryo (24) most strongly influences cell orientation whereas the electric field controls the direction of migration . It will be most interesting to determine whether currents of the appropriate magnitude and orientation to direct fibroblast migration exist around the neural tube at the time that these cells initiate their migration .…”

Observations on the migration and differentiation of neural crest cells in somite extirpated salamander larvae.

“…PRATT et al (1975) suggested that the accumulation of hyaluronic acid expanded the space through which neural crest cells migrated, and WESTON et al (1977WESTON et al ( , 1978 and DERBY (1978) suggested that the decrease in the concentration of hyaluronic acid narrowed this space and induced the aggregation of neural crest cells to form spinal ganglia. Under the present experimental conditions, there was no structure to block the migration of neural crest cells to the It has been claimed that scaffoldings for the migration of neural crest cells might be the basal lamina of the neural tube and the epidermis and fibronectin on it (NEWGREEN and THIERY, 1980;LOFBERG et al, 1980LOFBERG et al, , 1985THIERY et al, 1982). In the somite-free environment, scaffolding for the neural crest cells forming the cell cord was only on the basal lamina of the neural tube, so that they are associated with the lateral surface of the neural tube more closely than on the control side.…”

“…In the somite-free environment, scaffolding for the neural crest cells forming the cell cord was only on the basal lamina of the neural tube, so that they are associated with the lateral surface of the neural tube more closely than on the control side. However, it has been previously established that neural crest cells have a close association to the neural tube from the onset of migration (TAKAHASHI and YAMADORI, 1979;LOFBERG et al, 1980;HIRANO andSHIRAI, 1984, 1986) neural tube or fibronectin on it. The basal lamina of the epidermis must have existed in the present experiment, but for unknown reasons, no relationship was recognized between the lateral migration of neural crest cells and epidermis.…”