Neural cograft stimulates the survival and differentiation of embryonic stem cells in the adult mammalian auditory system

Hu, Zhengqing; Andäng, Michael; Ni, Duanyu; Ulfendahl, Mats

doi:10.1016/j.brainres.2005.06.016

Cited by 56 publications

(42 citation statements)

References 30 publications

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“…Dorsal root ganglion neurons co-grafted with mESCs would appear to promote migration and survival of the undifferentiated cells. In this experiment, mESCs were found to migrate into the SGN area via intrascalar access, suggesting that DRG co-grafts not only release growth factors to support the survival but also provide a structural matrix for cell growth in the fl uid-fi lled compartment (Hu et al 2005a ). Cells transplanted in the scala tympani are thought to migrate to the modiolus via holes located in the internal bony wall, known as ' canaliculae perforantes' .…”

Section: Intra-perilymphatic Transplantationmentioning

confidence: 88%

The Development of a Stem Cell Therapy for Deafness

Jongkamonwiwat¹,

Abbas²,

Barrott³

et al. 2016

Regenerative Medicine - From Protocol to Patient

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Medicine is at the doorstep of a phenomenal revolution, brought about by the advances in the stem cell fi eld and the development of new technologies to engineer cells and tissues into more complex organs. The promise of a true regenerative approach to organ damage and loss of function is closer than ever to becoming a reality. The auditory fi eld is participating in these developments with high expectations. Since the cochlea is an organ of diffi cult access and with very limited regenerative capacity, conventional therapeutic approaches have failed and, currently, the only treatments available are in the form of hearing aids and cochlear implants. The potential restoration of hearing by the use of exogenous stem cells will offer a solution to a condition that has very limited options. In this chapter, we review the increasing volume of research on this emerging fi eld and discuss the key elements that need to be developed further, in order to translate the basic science into a clinical reality.

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Section: Intra-perilymphatic Transplantationmentioning

confidence: 88%

The Development of a Stem Cell Therapy for Deafness

Jongkamonwiwat¹,

Abbas²,

Barrott³

et al. 2016

Regenerative Medicine - From Protocol to Patient

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“…Cochlear cultures, at various stages of differentiation, may be labeled and injected into the scala media, as was shown by Hildebrand et al [13] . Several studies have also shown that surgical transplantation of embryonic stem cells [27] , partially differentiated embryonic stem cells [13] and adult neural stem cells [28] into adult mammalian inner ear is not only possible, but also leads to further differentiation of those cells. Furthermore, Hu et al [27] showed that embryoid spheres can still be derived from dissected cochleae up to 240 h after death, making the feasibility of cadaveric transplants that much higher.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have also shown that surgical transplantation of embryonic stem cells [27] , partially differentiated embryonic stem cells [13] and adult neural stem cells [28] into adult mammalian inner ear is not only possible, but also leads to further differentiation of those cells. Furthermore, Hu et al [27] showed that embryoid spheres can still be derived from dissected cochleae up to 240 h after death, making the feasibility of cadaveric transplants that much higher. Further yet, Martinez-Monedero et al [29] showed reinnervation of hair cells by stem cell-derived neuronal progenitors, meaning that if successfully implanted, hair cells may also be successfully innervated through endogenous mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…The adult stem cell approach offers many unique opportunities, including autologous transplants, which avoid many of the risks inherent in other types of transplants. The feasibility of producing noncochlear hair cell progenitors has been supported by reports of other investigators, including successful induction of noncochlear adult stem cells (bone marrow stem cells) into a hair cell type fate [14] and survival of partially differentiated embryonic stem cells in the cochlea [13,15] . However, it seems likely that stem cells endogenous to the inner ear are primed/endowed to differentiate into hair cells more readily and faithfully than other stem cell types.…”

Section: Introductionmentioning

confidence: 98%

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Identification and Characterization of Mouse Cochlear Stem Cells

et al. 2006

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Genetic, noise- and drug-induced loss of hair cells in the mouse and human cochlea leads to permanent hearing loss due to lack of regeneration of hair cells, which may be due to reduced numbers or loss of the regenerative ability of stem cells in the adult cochlea. We hypothesized that the mouse neonate cochlea harbors stem cells capable of differentiating into hair cells. Cells from the primary neonate cochlear culture began to proliferate and formed floating spheres after 14 days in vitro (DIV). By comparison, spheres from the primary culture of the cortex were observed after 7 DIV. Cochlear sphere cells could be passaged and the new spheres were observed after 7 DIV. Cochlear sphere cells were capable of differentiating into astrocytes and oligodendrocytes, but not neurons under the conditions tested. Cochlear sphere cells expressed Sox2 and Myo7a, but failed to show markers that are expressed exclusively in mature cochlear tissue, while cells from cortex spheres expressed Sox2 and Otx2, but not Myo7a. Our results show that cochleae from neonatal mice harbor cells capable of forming spheres and cells from these spheres appear to be better endowed to become hair cells.

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“…We review previous reports and discuss obstacles to overcome for successful functional recovery. Several kinds of pluripotent stem cells have been delivered into the cochlea for the regeneration of SGNs, including NSCs [10,13,46], ESCs [4,5,11,12,29,34,36,38], bone marrow stem cells (BMSCs) [26,28,40], and iPSCs [27]. Tamura et al, evaluated the ability of NSCs to achieve neural differentiation in the modiolus of the cochlea and demonstrated that some grafted NSCs expressed β-III tubulin, a neuronal marker, although the majority of them differentiated into glial cells [46].…”

Section: Regeneration Of Spiral Ganglion Neuronsmentioning

confidence: 99%