Neural circuitry linking mating and egg laying in Drosophila females

Wang, Fei; Wang, Kaiyu; Forknall, Nora; Patrick, Christopher; Yang, Tansy; Parekh, Ruchi; Bock, Davi D.; Dickson, Barry J.

doi:10.1038/s41586-020-2055-9

Cited by 135 publications

(218 citation statements)

References 36 publications

Supporting

Mentioning

208

Contrasting

Order By: Relevance

“…3C) -including all 5 pC1 types found from manual tracing in the same EM volume (Wang et al, 2020). Two types have a ring dorsal to the lateral junction ('pC1-Las-a/b'; Las for Lasso; similar to pC1a,b in Wang et al 2020), two have a long medial projection ('pC1-Med-a,b'; Med for Medial; pC1-Med-a is similar to pC1c in Wang et al 2020), one has dense processes mediodorsal to the lateral junction (pC1e; same as in Wang et al 2020). Two other pC1 types have a horizontal projection medial to the 'ring' , similar to pC1 cells previously named 'pC1-Alpha' (Wu et al, 2019).…”

Section: Pc1 Cell Typesmentioning

confidence: 99%

“…We termed these 'pC1-Alpha-l/s' for long/short based on the length of the medial vertical projection (Fig. 3C, red arrows; pC1-Alpha-l is similar to pC1d in Wang et al 2020). The pC1-Int intersection labels ~7 cells per hemisphere in the brain, and includes the pC1-Alpha-l medial projection; it also has expression in the VNC (Supp Fig.…”

Section: Pc1 Cell Typesmentioning

confidence: 99%

“…(C) 7 pC1 cell types. pC1-Las-a,b (Las for 'Lasso'), pC1-Med-a,b (differ in their medial projection; 'Med' for medial, they both have a long medial projection), pC1-Alpha-s,l (short and long medial projections (red arrows) and pC1e (named as in Wang et al 2020).…”

Section: Pc1 Cell Typesmentioning

confidence: 99%

“…Names in parentheses are the ones used in Wang et al, 2020. (D) pC1-A (left; VT25602.AD; VT2064.DBD; n = 7, 2 ± 0 cells per hemisphere (Wu et al, 2019)) pC1-S (right; R71G01.AD;…”

Section: Pc1 Cell Typesmentioning

confidence: 99%

“…Lastly, we looked at the synaptic connectivity between pC1-Alpha and aIP-g cells in a second EM database that consists of a portion of the adult female brain (the 'hemibrain'; (Xu et al, 2020)), and found a set of 13 neurons identified as aIP-g ( (Xu et al, 2020); in this dataset a single pC1-Alpha has been traced, and is named pC1d (Wang et al 2020)). These aIP-g cells (denoted as types aIP-g-1-4 in the hemibrain) all share the aIP-g-c morphology (Supp Fig.…”

Section: And Suppmentioning

confidence: 99%

See 4 more Smart Citations

The Neural Basis for a Persistent Internal State inDrosophilaFemales

Deutsch

Pacheco

Encarnacion-Rivera

et al. 2020

Preprint

View full text Add to dashboard Cite

Sustained changes in mood or action require persistent changes in neural activity, but it has been difficult to identify and characterize the neural circuit mechanisms that underlie persistent activity and contribute to long-lasting changes in behavior. Here, we focus on changes in the behavioral state of Drosophila females that persist for minutes following optogenetic activation of a single class of central brain neurons termed pC1. We find that female pC1 neurons drive a variety of persistent behaviors in the presence of males, including increased receptivity, shoving, and chasing. By reconstructing cells in a volume electron microscopic image of the female brain, we classify 7 different pC1 cell types and, using cell type specific driver lines, determine that one of these, pC1-Alpha, is responsible for driving persistent female shoving and chasing. Using calcium imaging, we locate sites of minutes-long persistent neural activity in the brain, which include pC1 neurons themselves. Finally, we exhaustively reconstruct all synaptic partners of a single pC1-Alpha neuron, and find recurrent connectivity that could support the persistent neural activity. Our work thus links minutes-long persistent changes in behavior with persistent neural activity and recurrent circuit architecture in the female brain.

show abstract

Section: Pc1 Cell Typesmentioning

confidence: 99%

Section: Pc1 Cell Typesmentioning

confidence: 99%

Section: Pc1 Cell Typesmentioning

confidence: 99%

“…Names in parentheses are the ones used in Wang et al, 2020. (D) pC1-A (left; VT25602.AD; VT2064.DBD; n = 7, 2 ± 0 cells per hemisphere (Wu et al, 2019)) pC1-S (right; R71G01.AD;…”

Section: Pc1 Cell Typesmentioning

confidence: 99%

Section: And Suppmentioning

confidence: 99%

See 3 more Smart Citations

The Neural Basis for a Persistent Internal State inDrosophilaFemales

Deutsch

Pacheco

Encarnacion-Rivera

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

Sex peptide receptor mediates the post‐mating switch in Helicoverpa armigera (Lepidoptera: Noctuidae) female reproductive behavior

Liu

et al. 2021

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: In Drosophila, the sex peptide receptor (SPR) in females to detect male sex peptide (SP) and trigger changes in female behavior including mating rejection and oviposition. In moths, the SPR had been identified and investigated the limited function by using RNA interference (RNAi). However, the detailed function of SPR after a normal mating in moths, especially the role in female sexual receptivity, remains to be elucidated.RESULTS: In this study, we use Helicoverpa armigera, an economically important pest worldwide, as a case study to verify the function of SPR in vivo by CRISPR/Cas9 gene editing system. Analyzing the post-mating behaviors in mutant females, we investigated that HarmSPR could mediate the long-term sexual receptivity (remating, sex pheromone production and calling behavior) switch in female, but not affect the short-term sexual receptivity and the duration time of remating. The oviposition of mated HarmSPR −/− females was significantly decreased by ∼70% comparing to wild-type (WT) mated females and there were more eggs staying in the ovary of mutant females. SPR also showed effect on female longevity, which is that the mated HarmSPR −/− females showed longer longevity than mated WT females. CONCLUSION:The results detailedly demonstrated the functional role of SPR in female post-mating behaviors and we could propose a pest control strategy in which developing an antagonist of SPR to reduce the oviposition of female.

show abstract

Let's talk about sex: Mechanisms of neural sexual differentiation in Bilateria

Roggenbuck,

Hall,

Hanson

et al. 2024

WIREs Mechanisms of Disease

View full text Add to dashboard Cite

In multicellular organisms, sexed gonads have evolved that facilitate release of sperm versus eggs, and bilaterian animals purposefully combine their gametes via mating behaviors. Distinct neural circuits have evolved that control these physically different mating events for animals producing eggs from ovaries versus sperm from testis. In this review, we will describe the developmental mechanisms that sexually differentiate neural circuits across three major clades of bilaterian animals–Ecdysozoa, Deuterosomia, and Lophotrochozoa. While many of the mechanisms inducing somatic and neuronal sex differentiation across these diverse organisms are clade‐specific rather than evolutionarily conserved, we develop a common framework for considering the developmental logic of these events and the types of neuronal differences that produce sex‐differentiated behaviors.This article is categorized under: Congenital Diseases > Stem Cells and Development Neurological Diseases > Stem Cells and Development

show abstract

Neural circuitry linking mating and egg laying in Drosophila females

Cited by 135 publications

References 36 publications

The Neural Basis for a Persistent Internal State inDrosophilaFemales

The Neural Basis for a Persistent Internal State inDrosophilaFemales

Sex peptide receptor mediates the post‐mating switch in Helicoverpa armigera (Lepidoptera: Noctuidae) female reproductive behavior

Let's talk about sex: Mechanisms of neural sexual differentiation in Bilateria

Contact Info

Product

Resources

About