Neural activities in music frogs reveal call variations and phylogenetic relationships within the genus Nidirana

Abstract

The characteristics of acoustic signals co-evolve with preferences of the auditory sensory system. However, how the brain perceives call variations and whether it can reveal phylogenetic relationships among signalers remains poorly understood. Here, we recorded the neural signals from the Emei music frogs (Nidirana daunchina) in response to broadcasted calls of five different species of the same genus. We found that responses in terms of the different amplitudes of various event-related potential (ERP) components were correlated with diversification trends in acoustic signals, as well as phylogenetic relationships between N. daunchina and heterospecific callers. Specifically, P2 decreased gradually along the ordinal decline of similarities in acoustic characteristics of calls compared with those from conspecifics. Moreover, P3a amplitudes showed increasing trends in correspondence with callers’ genetic distances from the subject species. These observations collectively support the view that neural activities in music frogs can reflect call variations and phylogenetic relationships within the genus Nidirana.