Neue Bifunktionelle Brückenfunktionen zur A1‐B29‐Verklammerung der beiden Insulinketten

Abstract

Novel Bifunctional Bridging Functions for A1‐B29 Crosslinking of the Two Insulin Chains The positions A1 and B29 of the insulin molecule are crosslinked by the new bifunctional groups U and V. These insulin derivatives are convertible by oxydative sulfitolysis into A1‐B29 crosslinked hexa‐S‐sulfonates, which recombine by tributylphosphan/O2 in high yields to the A1‐B29 insulin derivatives, correctly reconstructing the disulfide system, and from which the bridging function is smoothly removable by acidolysis. The example of the bridging function U demonstrates that this principle is also suitable for the combination of both isolated single chains.