Network pharmacology integrated with molecular docking reveals the anticancer mechanism of Jasminum sambac Linn. essential oil against human breast cancer and experimental validation by in vitro and in vivo studies

Lakshmi, S. Rama; Kamaraj, M.; Nithya, T. G.; Chidambaranathan, N.; Pushpalatha, G. Grace Lydial; Santhosh, P.; Balavaishnavi, Balasubramanian; Mahajan, Megha

doi:10.1007/s12010-023-04481-2

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…The main types of breast cancer are classified as in situ/noninvasive (benign) and invasive or infiltrative carcinoma (malignant) . Mammary carcinogenesis is believed as a multistep procedure involving a transition from standard breast to a benign proliferative breast disease to a ductal cancer in situ to penetrating ductal cancer. , Granzyme B (GrB) belongs to serine protease and cleaves nuclear membrane protein Lamin B, finally disrupting the integrity of the nuclear membrane . GrB is secreted by activated natural killer cells (NK)/cytotoxic T lymphocytes (CTL).…”

Section: Introductionmentioning

confidence: 99%

Novel Noninvasive Serum Biomarkers for Prompt Diagnosis of Breast Carcinoma

Afzal,

Noreen,

Aslam

et al. 2023

ACS Omega

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Immune cell infiltration is associated with improved prognosis in the microenvironment of breast cancer. The incidence of breast cancer in Pakistan is 2.5 times higher than that in neighboring countries of Asia, accounting for 34.6% of female cancers. The objectives of this study were to compare and determine apoptotic mediators and biomarkers for breast carcinoma, such as serum granzyme B, cytochrome C, and vitamin D by ELIZA and calcium spectrophotometrically. Study groups were differentiated into malignant breast disease G-I, benign proliferative breast disease G-II, and healthy control group G-III. The immune-related prognostic markers and therapeutic targets were determined through the interaction of proteins by molecular docking and AutoDock Vina software. The level of granzyme B and cyt C was higher in Group-I, -II, and -III. Likewise, the mean vitamin D level was greater in Group-I than those in other groups. Through SwissDock, the proteins granzyme B and cyt C with vitamin D, single amino acid residue MET34 (H-bond 2.75 Å), and ILE81(H-bond 2.092 Å) were revealed to actively participate in interactions. This study reveals granzyme B and cyt C as biomarkers for malignant breast disease and benign proliferative breast disease, while hypovitaminosis D and hypocalcemia are complications or comorbidities of breast cancer.

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Section: Introductionmentioning

confidence: 99%

Novel Noninvasive Serum Biomarkers for Prompt Diagnosis of Breast Carcinoma

Afzal,

Noreen,

Aslam

et al. 2023

ACS Omega

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Evaluation of Anticancer, Anti-Inflammatory and Antioxidant Potential of Jasminum sambac Linn. Essential Oil

Lakshmi,

Kamaraj,

Mahajan

et al. 2024

International Journal of Nutrition, Pharmacology, Neurological Diseases

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Objective: The current study evaluates the therapeutic efficacy of Jasminum sambac Linn. essential oil (JEO) by investigating its pharmacological potentials, specifically its anticancer (using MCF-7 cells), anti-inflammatory, antioxidant and antimicrobial activity. Methods: With the anti-inflammatory activity assessed as red blood cell membrane stabilization and protein denaturation assay), antioxidant properties tested in DPPH and ABTS assay, and antimicrobial activity screened through disc diffusion method, anti-cancer functions were probed using NCF-7 breast cancer cells. The treatment of these cells with acridine orange–ethidium bromide (AOEtBr) stain helped checking the incidence of apoptotic body formation in the nuclear compartments. Results: The trial treating JEO on peripheral blood mononuclear cells (PBMCs) evinced no signs of cytotoxic changes revealing that JEO is exclusively cancer-cell-specific in action provides added value to this study. Free-radical scavenging activity confirms the antioxidant properties as the anti-inflamatory action is vouched in respective assays. Additionally, JEO is found to exert antimicrobial activity against Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli, and the fungal yeast Candida albicans in disc diffusion assay stretching its manifold utility. Conclusion: Although the present results are convincing, it must be acknowledged that further research focusing on cellular and molecular mechanisms is necessary. Nevertheless, the positive cues gathered on the therapeutic attributes of JEO lend credence to the folk notion that jasmine flowers can be used as an effective medicament for breast ailments.

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Network pharmacology integrated with molecular docking reveals the anticancer mechanism of Jasminum sambac Linn. essential oil against human breast cancer and experimental validation by in vitro and in vivo studies

Cited by 2 publications

References 56 publications

Novel Noninvasive Serum Biomarkers for Prompt Diagnosis of Breast Carcinoma

Novel Noninvasive Serum Biomarkers for Prompt Diagnosis of Breast Carcinoma

Evaluation of Anticancer, Anti-Inflammatory and Antioxidant Potential of Jasminum sambac Linn. Essential Oil

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