Network pharmacology-based analysis of the mechanism ofSaposhnikovia divaricatafor the treatment of type I allergy

Abstract

Context Saposhnikovia divaricata (Turcz.) Schischk (Apiaceae) (SD) has various pharmacological activities, but its effects on type I allergy (TIA) have not been comprehensively studied. Objective This study evaluates the treatment and molecular mechanisms of SD against TIA. Materials and methods The effective components and action targets of SD were screened using TCMSP database, and allergy-related targets of SD were predicted using GeneCards and OMIM database. The obtained target intersections were imported into David database for GO analysis, and used R software to perform KEGG analysis. The RBL-2H3 cells sensitised by DNP-IgE/DNP-BSA were treated with different concentrations of SD (root decoction, 0.5, 1, and 2 mg/mL), prim- O -glucosylcimifugin (POG, 10, 40, and 80 μg/mL) and the positive control drug–ketotifen fumarate (KF, 30 μM) for 12 h, then subjected to cell degranulation and qPCR analysis. Results Eighteen active compounds of SD and 38 intersection targets were obtained: TIA-related signal pathways mainly include calcium signal pathway, PI3K–Akt signal pathway and MAPK signal pathway. Taking the β-Hex release rate of the model group as the base, the release rate of SD and POG in high dose groups were 43.79% and 57.01%, respectively, which were significantly lower than model group ( p < 0.01), and significantly lower than KF group (63.83%, p < 0.01, p < 0.05). SD and POG could down-regulate the expression of related proteins in the Lyn/Syk, PI3K/AKT and MAPK signalling pathways. Discussion and conclusion Saposhnikovia divaricata could inhibit IgE-induced degranulation of mast cells, providing a scientific basis for further research and clinical applications of SD in TIA treatment.

Knowledge mapping of links between dendritic cells and allergic diseases: A bibliometric analysis (2004–2023)

“…S. divaricata is a herb that was demonstrated to have a positive effect on allergic diseases [ 43 , 44 ]. Cleomiscosin A [ [45] , [46] , [47] , [48] ], vanillic acid [ 45 , [49] , [50] , [51] , [52] , [53] ], prim- O -glucosylcimifugin [ 45 , [54] , [55] , [56] ], and 5- O -methylvisammioside [ 45 , 56 , 57 ] are bioactive components of S. divaricata and these components play an important role in inhibiting allergic inflammation. In innate and adaptive immunity, TLRs have developed into essential molecules [ 58 ].…”

“…According to more than one study, vanillic acid can decrease the expression of inflammatory markers incluing TLRs [ 86 , 87 ]. Though there was not the direct research between TLRs and the components of prim- O -glucosylcimifugin and 5- O -methylvisammioside, the function in anti-allergy of prim- O -glucosylcimifugin and 5- O -methylvisammioside has been reported more than once [ [53] , [54] , [55] , [56] ]. Molecular docking is established to discover new drug based on 3D structure in silico [ 88 ].…”

Short-time ozone treatment promotes protease-mediated destruction of B cell allergen epitopes by altering the structural characteristics of whey protein

“…β-hex represents a distinctive marker particle found pre-existing in mast cells and basophils. 36 Apart from histamine and β-hex, IL-6 also plays a crucial role in immune regulation. Under normal circumstances, IL-6 is expressed at low levels in the human body, acting protectively against corresponding tissue damage.…”

Hawthorn or semen cassiae-alleviated high-fat diet-induced hepatic steatosis in rats via the reduction of endoplasmic reticulum stress

“…Therein, the same target molecules and signaling pathways were identified through network pharmacological analysis, which is an effective tool for the systematic analysis of pharmacodynamic substances and mechanism of action of TCMs. The holistic and systematic approach of network pharmacology, 22,23 focusing on drug-drug interactions, is in line with multitarget and multipathways characteristic of TCMs, and is an effective tool for the systematic analysis of pharmacodynamics substances and mechanisms of action of TCMs. In our report, PPAR-γ, screened by TCMSP, was highly expressed in the liver of rats with hepatic steatosis, and its expression was greatly blocked by hawthorn/semen cassiae.…”