Network pharmacology-based analysis and experimental in vitro validation on the mechanism of Paeonia lactiflora Pall. in the treatment for type I allergy

Zhao, Yang; Li, Hui; Li, Xiangsheng; Sun, Yizhao; Shao, Yuxin; Zhang, Yanfen; Liu, Zhongcheng

doi:10.1186/s12906-022-03677-z

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…In contrast, Western medicine typically relies on symptomatic supportive drug interventions for dysmenorrhea, such as ibuprofen extended-release capsules, a type of non-steroidal anti-inflammatory drug. While these medications can alleviate pain symptoms, their efficacy tends to be short-lived, and prolonged use may lead to adverse reactions [4] . According to the principles of Chinese medicine, dysmenorrhea is categorized as menstrual abdominal pain, primarily attributed to cold and damp stagnation.…”

Section: Discussionmentioning

confidence: 99%

Clinical Effect of Warm Acupuncture and Moxibustion Combined with Traditional Chinese Herbs in Treating Cold-Damp Stagnation Dysmenorrhea

Yang

2024

AOGR

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Objective: To investigate the clinical effect of warm acupuncture and moxibustion with traditional Chinese herbs in treating cold-damp stagnation dysmenorrhea. Methods: 76 patients with cold-damp stagnation dysmenorrhea admitted to our hospital were selected as the sample for study and evaluation, and the time of admission to the hospital was from January–December 2023. The patients were divided into an observation group (n = 38) and a control group (n = 38) using a random number table. The patients in the control group were treated with conventional Western analgesic drugs. In contrast, the patients in the observation group were treated with warm acupuncture and moxibustion together with traditional Chinese herbs. The clinical effective rate, traditional Chinese medicine (TCM) symptom score, NRS score, serum factor level, and the incidence rate of adverse reactions were compared between the two groups. Results: The clinical efficacy of the observation group was higher than that of the control group (P < 0.05); the TCM symptom score of the observation group was lower than that of the control group (P < 0.05); the PGF2a and NRS scores of the observation group were lower than that of the control group, while the PGE2 was higher than that of the control group (P < 0.05); the incidence rate of adverse reactions of the observation group was lower than that of the control group (P＜0.05). Conclusion: Warm acupuncture and moxibustion combined with traditional Chinese herbs are effective in treating patients with cold-damp stagnation dysmenorrhea. This treatment regime alleviates clinical symptoms, reduces the degree of pain, improves the level of serum factor, and reduces the incidence of adverse reactions. Therefore, it should be popularized.

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Section: Discussionmentioning

confidence: 99%

Clinical Effect of Warm Acupuncture and Moxibustion Combined with Traditional Chinese Herbs in Treating Cold-Damp Stagnation Dysmenorrhea

Yang

2024

AOGR

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Phytochemical analysis, in-vitro and in-silico study of antiproliferative activity of ethyl acetate fraction of Launaea cornuta (Hochst. ex Oliv. & Hiern) C. Jeffrey against human cervical cancer cell line

Lagu,

Nyamai,

Njeru

2024

Front. Pharmacol.

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Introduction: Cervical cancer is one of the leading causes of death among women globally due to the limitation of current treatment methods and their associated adverse side effects. Launaea cornuta is used as traditional medicine for the treatment of a variety of diseases including cancer. However, there is no scientific validation on the antiproliferative activity of L. cornuta against cervical cancer.Objective: This study aimed to evaluate the selective antiproliferative, cytotoxic and antimigratory effects of L. cornuta and to explore its therapeutical mechanisms in human cervical cancer cell lines (HeLa-229) through a network analysis approach.Materials and methods: The cytotoxic effect of L. cornuta ethyl acetate fraction on the proliferation of cervical cancer cells was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) bioassay and the antimigratory effect was assessed by wound healing assays. Compounds were analysed using the qualitative colour method and gas chromatography-mass spectroscopy (GC-MS). Subsequently, bioinformatic analyses, including the protein-protein interaction (PPI) network analysis, Gene Ontology (GO), and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis, were performed to screen for potential anticervical cancer therapeutic target genes of L. cornuta. Molecular docking (MD) was performed to predict and understand the molecular interactions of the ligands against cervical cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the network analysis results.Results:L. cornuta ethyl acetate fraction exhibited a remarkable cytotoxic effect on HeLa-229 proliferation (IC50 of 20.56 ± 2.83 μg/mL) with a selectivity index (SI) of 2.36 with minimal cytotoxicity on non-cancerous cells (Vero-CCL 81 (IC50 of 48.83 ± 23.02). The preliminary screening revealed the presence of glycosides, phenols, saponins, terpenoids, quinones, and tannins. Thirteen compounds were also identified by GC-MS analysis. 124 potential target genes associated with the effect of L. cornuta ethyl acetate fraction on human cervical cancer were obtained, including AKT1, MDM2, CDK2, MCL1 and MTOR were identified among the top hub genes and PI3K/Akt1, Ras/MAPK, FoxO and EGFR signalling pathways were identified as the significantly enriched pathways. Molecular docking results showed that stigmasteryl methyl ether had a good binding affinity against CDK2, ATK1, BCL2, MDM2, and Casp9, with binding energy ranging from −7.0 to −12.6 kcal/mol. Tremulone showed a good binding affinity against TP53 and P21 with −7.0 and −8.0 kcal/mol, respectively. This suggests a stable molecular interaction of the ethyl acetate fraction of L. cornuta compounds with the selected target genes for cervical cancer. Furthermore, RT-qPCR analysis revealed that CDK2, MDM2 and BCL2 were downregulated, and Casp9 and P21 were upregulated in HeLa-229 cells treated with L. cornuta compared to the negative control (DMSO 0.2%).Conclusion: The findings indicate that L. cornuta ethyl acetate fraction phytochemicals modulates various molecular targets and pathways to exhibit selective antiproliferative and cytotoxic effects against HeLa-229 cells. This study lays a foundation for further research to develop innovative clinical anticervical cancer agents.

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Anti-Allergic and Anti-Inflammatory Signaling Mechanisms of Natural Compounds/Extracts in In Vitro System of RBL-2H3 Cell: A Systematic Review

Rana,

Bansode,

Williams

2024

Cells

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Various extracts are tested for anti-allergic or anti-inflammatory properties on in vitro models. RBL-2H3 cells are widely used in allergic or immunological studies. FCεRI and its downstream signaling cascades, such as MAPK, NF-κB, and JAK/STAT signaling pathways, are important allergic or inflammatory signaling mechanisms in mast and basophil cells. This systematic review aims to study common signaling pathways of the anti-allergic or anti-inflammatory compounds on RBL-2H3 cells. We selected the relevant research articles published after 2015 from the PubMed, Scopus, Science Direct and Web of Science databases. The risk of bias of the studies was assessed based on the modified CONSORT checklist for in vitro studies. The cell lines, treatments, assay, primary findings, and signaling pathways on RBL-2H3 cells were extracted to synthesize the results. Thirty-eight articles were included, and FCεRI and its downstream pathways, such as Lyn, Sky, PLCγ, and MAPK, were commonly studied. Moreover, the JAK/STAT pathway was a potential signaling mechanism in RBL-2H3 cells. However, the findings based on RBL-2H3 cells needed to be tested along with human mast cells to confirm its relevance to human health. In conclusion, a single plant extract may act as an anti-inflammatory reagent in RBL-2H3 cells via multiple signaling pathways besides the MAPK signaling pathway.

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Network pharmacology-based analysis and experimental in vitro validation on the mechanism of Paeonia lactiflora Pall. in the treatment for type I allergy

Cited by 3 publications

References 48 publications

Clinical Effect of Warm Acupuncture and Moxibustion Combined with Traditional Chinese Herbs in Treating Cold-Damp Stagnation Dysmenorrhea

Clinical Effect of Warm Acupuncture and Moxibustion Combined with Traditional Chinese Herbs in Treating Cold-Damp Stagnation Dysmenorrhea

Phytochemical analysis, in-vitro and in-silico study of antiproliferative activity of ethyl acetate fraction of Launaea cornuta (Hochst. ex Oliv. & Hiern) C. Jeffrey against human cervical cancer cell line

Anti-Allergic and Anti-Inflammatory Signaling Mechanisms of Natural Compounds/Extracts in In Vitro System of RBL-2H3 Cell: A Systematic Review

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