Network pharmacology analysis to explore mechanism of Three Flower Tea against nonalcoholic fatty liver disease with experimental support using high-fat diet-induced rats

Wu, Peixuan; Liang, Shufei; He, Ying; Lv, Rui; Yang, Bendong; Wang, Meng; Wang, Chao; Li, Yong; Song, Xinhua; Sun, Wenlong

doi:10.1016/j.chmed.2022.03.002

Cited by 12 publications

(7 citation statements)

References 30 publications

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“…It constructs the “component–target–pathway” network relationship to explore the targets of drugs and the possible biological functions involved, thereby systematically elucidating the mechanism of drug therapy. This coincides with the holistic treatment with multicomponent, multitarget and multi‐approach thinking in TCM (Chen et al, 2022; Hao & Xiao, 2014; Wu et al, 2022). Molecular docking is a recent technology developed from the “lock and key model”.…”

Section: Introductionsupporting

confidence: 74%

A comprehensive quality evaluation method of different medicinal parts of Physalis Calyx seu Fructus by fingerprints, chemometrics, antioxidant activity, network pharmacology and molecular docking

Liu

et al. 2023

Biomedical Chromatography

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Physalis Calyx seu Fructus (PCF) is a herb widely used in China for its function of clearing heat and detoxifying, benefitting the pharynx and reducing phlegm, both in health care and in tea drinking. However, the quality of its fruit and calyx is uneven and the storage period is short. Therefore, it is crucial to develop other parts of PCF with longer storage periods and obvious medicinal effects. Firstly, high‐performance liquid chromatography was used to develop the fingerprint of different parts of PCF, and various chemometric analyses were conducted to screen out chemical markers. The calyxes of PCF were found to cluster together, distinct from the fruits, roots, stems and leaves. The active components of PCF were concentrated in the persistent calyxes, and flavonoids were mainly found in the persistent calyxes and leaves. Secondly, the extraction of persistent calyxes showed the strongest scavenging ability of DPPH and ABTS. Finally, the important chemical markers were verified by network pharmacological analysis and molecular docking. It provides a reference for the clinical application of PCF, and the obtained chemical markers offer a scientific basis for quality evaluation.

show abstract

Section: Introductionsupporting

confidence: 74%

A comprehensive quality evaluation method of different medicinal parts of Physalis Calyx seu Fructus by fingerprints, chemometrics, antioxidant activity, network pharmacology and molecular docking

Liu

et al. 2023

Biomedical Chromatography

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show abstract

“…It can construct the "component-target-pathway" network relationship to explore the targets of drugs and the possible biological functions involved, and systematically elucidate the mechanism of drug therapy. This coincides with the holistic treatment thinking of multi-component, multi-target, and multi-approach in TCM (Hao et al 2014;Chen et al 2022; Wu et al 2022). Molecular docking is a new technology developed from the "lock and key model".…”

supporting

confidence: 77%

WITHDRAWN: A comprehensive quality evaluation method of different medicinal parts of Physalis Calyx seu Fructus by fingerprints, chemometrics, antioxidant activity, network pharmacology, and molecular docking

Liu

et al. 2022

Preprint

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Physalis Calyx seu Fructus (PCF) is one herb with the function of clearing heat and detoxify, benefiting pharynx and reducing phlegm, widely used in health care and tea drinking in China. However, the storage period of fruit and calyx was short and the quality was uneven. It is very important to further develop other parts with long storage period and obvious effect of medicine in PCF. Firstly, the fingerprint of differernt parts of PCF was developed by High-performance Liquid Chromatography (HPLC). The chemical markers were screened out by a variety of chemometric analyses. It was found that the calyxes of PCF could self-cluster among fruits, roots, stems, and leaves. It was found that the significant active components of PCF were mainly in persistent calyxes, and flavonoids were mainly in persistent calyxes and leaves. Moreover, the extraction of persistent calyxes had the strongest scavenging ability of DPPH and ABTS. Finally, the important chemical markers were verified by network pharmacological analysis and molecular docking. It provides a reference for clinical application of PCF, and the obtained chemical markers also provide a scientific reference for quality evaluation.

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“…NAFLD is a heterogeneous pathological state of liver, which is caused by immoderate infiltration of fat in liver cells under other factors except alcohol 1 . The disease progresses from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH), and finally to NASH-related cirrhosis 2 .…”

Section: Introductionmentioning

confidence: 99%

NUP85 alleviates lipid metabolism and inflammation by regulating PI3K/AKT signaling pathway in nonalcoholic fatty liver disease

Wu,

Yan,

Yue

et al. 2024

Int. J. Biol. Sci.

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Nonalcoholic fatty liver disease (NAFLD) is one of the common causes of chronic liver disease in the world. The problem of NAFLD had become increasingly prominent. However, its pathogenesis is still indistinct. As we all know, NAFLD begins with the accumulation of triglyceride (TG), leading to fatty degeneration, inflammation and other liver tissues damage. Notably, structure of nucleoporin 85 (NUP85) is related to lipid metabolism and inflammation of liver diseases. In this study, the results of researches indicated that NUP85 played a critical role in NAFLD. Firstly, the expression level of NUP85 in methionine-choline-deficient (MCD)-induced mice increased distinctly, as well as the levels of fat disorder and inflammation. On the contrary, knockdown of NUP85 had the opposite effects. In vitro , AML-12 cells were stimulated with 2 mm free fatty acids (FFA) for 24 h. Results also proved that NUP85 significantly increased in model group, and increased lipid accumulation and inflammation level. Besides, NUP85 protein could interact with C-C motif chemokine receptor 2 (CCR2). Furthermore, when NUP85 protein expressed at an extremely low level, the expression level of CCR2 protein also decreased, accompanied with an inhibition of phosphorylation of phosphoinositol-3 kinase (PI3K)-protein kinase B (AKT) signaling pathway. What is more, trans isomer (ISRIB), a targeted inhibitor of NUP85, could alleviate NAFLD. In summary, our findings suggested that NUP85 functions as an important regulator in NAFLD through modulation of CCR2.

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Network pharmacology analysis to explore mechanism of Three Flower Tea against nonalcoholic fatty liver disease with experimental support using high-fat diet-induced rats

Cited by 12 publications

References 30 publications

A comprehensive quality evaluation method of different medicinal parts of Physalis Calyx seu Fructus by fingerprints, chemometrics, antioxidant activity, network pharmacology and molecular docking

A comprehensive quality evaluation method of different medicinal parts of Physalis Calyx seu Fructus by fingerprints, chemometrics, antioxidant activity, network pharmacology and molecular docking

WITHDRAWN: A comprehensive quality evaluation method of different medicinal parts of Physalis Calyx seu Fructus by fingerprints, chemometrics, antioxidant activity, network pharmacology, and molecular docking

NUP85 alleviates lipid metabolism and inflammation by regulating PI3K/AKT signaling pathway in nonalcoholic fatty liver disease

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