Network pharmacology analysis and experimental validation to explore the mechanism of sea buckthorn flavonoids on hyperlipidemia

Xiao, Ping-Ting; Liu, Shiyu; Kuang, Yu-Jia; Jiang, Zheng‐Meng; Yang, Lin; Xie, Zhishen; Liu, E‐Hu

doi:10.1016/j.jep.2020.113380

Cited by 63 publications

(42 citation statements)

References 42 publications

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“…The target genes of gastrointestinal and respiratory disorders were sorted separately, corrected with Uniprot, and subjected to the VarElect tool to validate and score the genes. The top 200 genes were kept from gastrointestinal and respiratory disorder gene datasets [29,30]. The disease target genes and 269 compound target genes were intersected in the Venn diagram, revealing 57 intersected potential targets for gastrointestinal and respiratory disorders (Figure S1).…”

Section: Network Pharmacology Analysismentioning

confidence: 99%

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

et al. 2021

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Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. C. sativus seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K+ (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, C. sativus seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.

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Section: Network Pharmacology Analysismentioning

confidence: 99%

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

et al. 2021

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“…It was found that FSBT could alleviate high-fat and highfructose diet (HFFD)-induced cognitive impairment in mice by effectively normalizing insulin signaling and neuroinflammation (Mulati et al, 2020). Xiao et al confirmed that FSBT dose-dependently promoted cholesterol transformation into bile acids and cholesterol efflux, inhibited cholesterol de novo synthesis and accelerated fatty acids oxidation to ameliorate hyperlipidemia (Xiao et al, 2020).…”

Section: Lipid Metabolismmentioning

confidence: 99%

Flavonoids from sea buckthorn: A review on phytochemistry, pharmacokinetics and role in metabolic diseases

et al. 2021

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Sea buckthorn (Hippophae rhamnoides L., SBT) is being used as a folk medicine for their diverse medicinal properties. Flavonoids are generally considered as the main bioactive and characteristic ingredients in SBT. This review was conducted using a comprehensive literature search on the chemical components, quality control, pharmacokinetics of flavonoids from SBT (FSBT). Particularly, we highlighted the therapeutic potential in metabolic diseases and clinical applications of FSBT. More than 95 flavonoids have been identified from SBT. Although the oral bioavailability of FSBT was relatively low, FSBT displays significant effect on the regulation of metabolism to ameliorate metabolic disorders and their complications. There is a heightened need to explore the bioactive compounds in SBT and mechanism(s) of action of FSBT in order to fully understand the pathways of their activities. Practical applicationsFor years, due to the increasing emergence of metabolic syndrome and diverse functions of FSBT in regulating metabolism, they can be efficiently utilized for human health and have an urgent need to become a hotspot for research. This review will broaden the understanding of FSBT, providing some directions for further development and expanding the therapeutic applications of FSBT.

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“…Sea buckthorn berries are popular in pharmaceutical manufacturing due to a large number of active ingredients. It is abundant in vitamins, amino acids, and flavonoids ( Mihalcea et al, 2018 ; Li et al, 2016 ; Xiao et al, 2021 ). Researchers have shown that the content and activity of active substances in sea buckthorn berries grown in different environments differ, with the berries of sea buckthorn grown at higher altitudes being of better quality than those grown in the plains ( Guo et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Composition analysis and antioxidant activity evaluation of a high purity oligomeric procyanidin prepared from sea buckthorn by a green method

Zhu

Yuen²,

Li³

et al. 2021

Current Research in Food Science

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Procyanidin is an important polyphenol for its health-promoting properties, however, the study of procyanidin in sea buckthorn was limited. In this paper, sea buckthorn procyanidin (SBP) was obtained through a green isolation and enrichment technique with an extraction rate and purity of 9.1% and 91.5%. The structure of SBP was analyzed using Ultraviolet–visible spectroscopy (UV–vis), Fourier-transform infrared spectroscopy (FT-IR), and liquid chromatography-mass spectrometry (LC-MS/MS). The results show that SBP is an oligomeric procyanidin, mainly composed of (−)-epicatechin gallate, procyanidin B, (+)-gallocatechin-(+)-catechin, and (+)-gallocatechin dimer. SBP showed superior scavenging capacity on free radicals. Furthermore, the cleaning rate of the ABTS radical was 4.8 times higher than vitamin C at the same concentration. Moreover, SBP combined with vitamin C presented potent synergistic antioxidants with combined index values below 0.3 with concentration rates from 5:5 to 2:8. SBP also provided significant protection against oxidative stress caused by hydrogen peroxide (H 2 O 2 ) on RAW264.7 cells. These findings prove the potential of SBP as a natural antioxidant in food additives and support the in-depth development of sea buckthorn resources.

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Network pharmacology analysis and experimental validation to explore the mechanism of sea buckthorn flavonoids on hyperlipidemia

Cited by 63 publications

References 42 publications

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

Flavonoids from sea buckthorn: A review on phytochemistry, pharmacokinetics and role in metabolic diseases

Composition analysis and antioxidant activity evaluation of a high purity oligomeric procyanidin prepared from sea buckthorn by a green method

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